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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- MA5-31726 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- eIF5 Monoclonal Antibody (6D6H11)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 6D6H11
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of eIF5 in human EIF5 recombinant protein. Sample was incubated with eIF5 monoclonal antibody (Product # MA5-31726) using a dilution of 1:500-1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of eIF5 in HEK293 (1) and EIF5 cell lysate. Samples were incubated with eIF5 monoclonal antibody (Product # MA5-31726) using a dilution of 1:500-1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ELISA analysis of eIF5 in Control Antigen (black line, 100 ng); Antigen (purple line, 10 ng); Antigen (blue line, 50 ng); Antigen (red line, 100 ng). Samples were incubated with eIF5 monoclonal antibody (Product # MA5-31726) using a dilution of 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of eIF5 in Hela cells (green). Sample was incubated with eIF5 monoclonal antibody (Product # MA5-31726) using a dilution of 1:50-1:250 followed by DRAQ5 fluorescent DNA dye (blue), and Alexa Fluor- 555 phalloidin (red labeled actin filaments) .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of eIF5 in paraffin-embedded bladder cancer tissue. Sample was incubated with eIF5 monoclonal antibody (Product # MA5-31726) using a dilution of 1:200-1:1000 followed by DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of eIF5 in paraffin-embedded rectum cancer tissue. Sample was incubated with eIF5 monoclonal antibody (Product # MA5-31726) using a dilution of 1:200-1:1000 followed by DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of eIF5 in HeLa cells (green). Sample was incubated with eIF5 monoclonal antibody (Product # MA5-31726) using a dilution of 1:200-1:400 followed by negative control (red).
