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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunohistochemistry [1]
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- Product number
- PA5-30223 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- eIF5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: U87-MG, mouse brain, rat brain. Predicted reactivity: Mouse (97%), Rat (97%), Chicken (92%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- eIF5 Polyclonal Antibody detects EIF5 protein by western blot analysis. A. 50 µg mouse brain lysate/extract.10% SDS-PAGE. EIF5 Polyclonal Antibody (Product # PA5-30223) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using eIF5 Polyclonal Antibody (Product # PA5-30223). Sample (30 µg of whole cell lysate). Lane A: U87-MG. 10% SDS PAGE. eIF5 Polyclonal Antibody (Product # PA5-30223) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- eIF5 Polyclonal Antibody detects EIF5 protein by western blot analysis. A. 50 µg mouse brain lysate/extract.10% SDS-PAGE. EIF5 Polyclonal Antibody (Product # PA5-30223) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- eIF5 Polyclonal Antibody detects EIF5 protein by western blot analysis. A. 50 µg rat brain lysate/extract.10% SDS-PAGE. EIF5 Polyclonal Antibody (Product # PA5-30223) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using EIF5 (Product # PA5-30223) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
