A305-655A-T

antibody from Invitrogen Antibodies

Targeting: S100A16 DT1P1A7, MGC17528, S100F

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A305-655A-T

Provider

Invitrogen Antibodies

Product name

S100A16 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

10 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human S100A16 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, MCF-7, Jurkat, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-S100A16 antibody (Product # A305-655A-T; lot A305-655A-T-2) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human S100A16 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-S100A16 antibody A305-655A-M (lot A305-655A-M-1) used for IP at 20 µl per reaction. S100A16 was also immunoprecipitated by rabbit anti-S100A16 antibody A305-654A-M. For blotting immunoprecipitated S100A16, A305-655A-M was used at 1:100. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human S100A16 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-S100A16 antibody A305-655A-M (lot A305-655A-M-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human S100A16 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-S100A16 antibody (Product # A305-655A-T (lot A305-655A-T-2) used for IP at 20 µL per reaction. S100A16 was also immunoprecipitated by a previous lot of this antibody (lot A305-655A-T-1) and rabbit anti-S100A16 antibody (Product # A305-654A-T). For blotting immunoprecipitated S100A16, Product # A305-655A-T was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.