Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB8074 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Intelectin-1/Omentin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse Intelectin-1/Omentin in ELISA. In direct ELISAs, no significant cross-reactivity with recombinant mouse Intelectin-2 and recombinant human Intelectin-1 is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
O88310- Isotype
- IgG
- Antibody clone number
- 746420
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse Intelectin-1/Omentin by Western Blot. Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Intelectin-1/Omentin Monoclonal Antibody (Catalog # MAB8074) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Intelectin-1/Omentin at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Intelectin-1/Omentin in Mouse Intestine. Intelectin-1/Omentin was detected in perfusion fixed frozen sections of mouse intestine using Rat Anti-Mouse Intelectin-1/Omentin Monoclonal Antibody (Catalog # MAB8074) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to Paneth cells in intestinal glands. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
