PA5-107159

antibody from Invitrogen Antibodies

Targeting: LRRC52 FLJ25811

Western blot (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-107159

Provider

Invitrogen Antibodies

Product name

LRRC52 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Sequence homology: Cow: 92%; Dog: 75%; Guinea Pig: 83%; Horse: 75%; Human: 100%; Mouse: 83%; Pig: 92%; Rabbit: 93%; Rat: 92%

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Homozygous mutation in SLO3 leads to severe asthenoteratozoospermia due to acrosome hypoplasia and mitochondrial sheath malformations.

Lv M, Liu C, Ma C, Yu H, Shao Z, Gao Y, Liu Y, Wu H, Tang D, Tan Q, Zhang J, Li K, Xu C, Geng H, Zhang J, Li H, Mao X, Ge L, Fu F, Zhong K, Xu Y, Tao F, Zhou P, Wei Z, He X, Zhang F, Cao Y
Reproductive biology and endocrinology : RB&E 2022 Jan 3;20(1):5

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 6 Membrane potential and expression patterns of LRRC52 and CatSper1 examined in the spermatozoa from control and SLO3 -mutant subjects. A Membrane potential measurements of wild-type and SLO3 mutant sperm in capacitated conditions. The capacitation-associated hyperpolarization of human sperm is inhibited in SLO3 mutant spermatozoa, Data are presented as the mean +- SEM. *** P < 0.001; Student's t -test. B Immunoblotting of sperm lysates from controls and the SLO3 -mutated individual using the anti-LRRC52 and CatSper1 antibodies. Na + /K + -ATPase alpha1 was used as loading control. The expression of the LRRC52 protein is decreased in mutant sperm, whereas the level of the CatSper1 protein is comparable to that of control. C Representative images of spermatozoa from fertile controls and the SLO3 mutant patient stained with the anti-LRRC52 antibody, anti-CatSper1 antibody, anti-Ac-tubulin antibody, and Hoechst. Two independent experiments were performed and at least 150 sperm we re-examined for each time per individual. Scale bars, 20 mum