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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
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- Product number
- PA5-52173 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DIAPH2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: GGSEEPGGGR SNKRSAGNRA ANEEETKNKP KLNIQIKTLA DDVRDRITSF RKSTVKKEKP LIQHPIDSQV AMSEFPAAQP LYDERSLNLS EKEVLDLFEK MMEDMNLNEE KKAPLRNKDF TTKREMVVQY ISAT Highest antigen sequence identity to the following orthologs: Mouse - 82%, Rat - 39%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DIAPH2 in Lane 1: Marker (kDa) 220, 112, 84, 47, 32, 26, 17; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a DIAPH2 Polyclonal Antibody (Product # PA5-52173).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DIAPH2 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a DIAPH2 Polyclonal Antibody (Product # PA5-52173).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of DIAPH2 in human cell line U-251 MG shows positivity in nucleoli, the Golgi apparatus & vesicles. Samples were probed using a DIAPH2 Polyclonal Antibody (Product # PA5-52173).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of DIAPH2 in human cell line U-251 MG using a DIAPH2 Polyclonal Antibody (Product # PA5-52173) shows localization to nucleoli, endoplasmic reticulum and vesicles.
