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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [6]
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- Product number
- MA5-24625 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DIAPH2 Monoclonal Antibody (CL1113)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: GGSEEPGGGR SNKRSAGNRA ANEEETKNKP KLNIQIKTLA DDVRDRITSF RKSTVKKEKP LIQHPIDSQV AMSEFPAAQP LYDERSLNLS EKEVLDLFEK MMEDMNLNEE KKAPLRNKDF TTKREMVVQY ISAT Highest antigen sequence identity to the following orthologs: Mouse - 82%, Rat - 33%. Binds to an epitope located within the peptide sequence IKTLADDVRDRITSF as determined by overlapping synthetic peptides.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- CL1113
- Vial size
- 100 µL
- Concentration
- 0.7 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DIAPH2 in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a DIAPH2 Monoclonal Antibody (CL1113) (Product # MA5-24625). Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DIAPH2 in human testis tissue shows strong cytoplasmic immunoreactivity in seminiferous tubules cells. Samples were probed using a DIAPH2 Monoclonal Antibody (Product # MA5-24625).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DIAPH2 in human uterus tissue shows strong cytoplasmic immunoreactivity in glandular cells. Samples were probed using a DIAPH2 Monoclonal Antibody (Product # MA5-24625).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DIAPH2 in human kidney tissue shows moderate positivity in renal glomeruli. Samples were probed using a DIAPH2 Monoclonal Antibody (Product # MA5-24625).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DIAPH2 in human colorectal cancer shows strong cytoplasmic immunoreactivity in tumor cells. Samples were probed using a DIAPH2 Monoclonal Antibody (Product # MA5-24625).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DIAPH2 in human placenta shows strong staining of the trophoblast. Samples were probed using a DIAPH2 Monoclonal Antibody (Product # MA5-24625).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DIAPH2 in human pancreatic tissue shows absence of immunoreactivity (negative control). Samples were probed using a DIAPH2 Monoclonal Antibody (Product # MA5-24625).
