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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- ACL-031-25UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PACC1/TMEM206 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain lysate (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and rat kidney lysates (lanes 3 and 6): - 1-3. Anti-PACC1/TMEM206 (extracellular) Antibody (#ACL-031), (1:200). 4-6. Anti-PACC1/TMEM206 (extracellular) Antibody , preincubated with PACC1/TMEM206 (extracellular) Blocking Peptide (BLP-CL031).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human U-87 MG glioblastoma cell line lysate (lanes 1 and 5), human HT-29 colon adenocarcinoma cell line lysate (lanes 2 and 6), human THP-1 monocytic leukemia cell line lysate (lanes 3 and 7) and mouse BV-2 microglia cell line lysate (lanes 4 and 8): - 1-4. Anti-PACC1/TMEM206 (extracellular) Antibody (#ACL-031), (1:200). 5-8. Anti-PACC1/TMEM206 (extracellular) Antibody , preincubated with PACC1/TMEM206 (extracellular) Blocking Peptide (BLP-CL031).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of PACC1 in mouse reticular thalamic nucleus. Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-PACC1/TMEM206 (extracellular) Antibody (#ACL-031), (1:200), followed by goat Anti-rabbit-AlexaFluor-488. A. PACC1 immunoreactivity (green) appears in neurons (arrows). B. Pre-incubation of the Antibody with PACC1/TMEM206 (extracellular) Blocking Peptide (BLP-CL031), suppressed staining. Cell nuclei are stained with DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of PACC1 in mouse reticular thalamic nucleus. Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-PACC1/TMEM206 (extracellular) Antibody (#ACL-031), (1:200), followed by goat Anti-rabbit-AlexaFluor-488. A. PACC1 immunoreactivity (green) appears in neurons (arrows). B. Pre-incubation of the Antibody with PACC1/TMEM206 (extracellular) Blocking Peptide (BLP-CL031), suppressed staining. Cell nuclei are stained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of PACC1 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-PACC1/TMEM206 (extracellular) Antibody (#ACL-031), (2.5μg) + goat- Anti-rabbit-FITC.
