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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- GTX100292 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX100292, RRID:AB_1240895
- Product name
- GPX2 antibody [C1C3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Caffeic acid and resveratrol ameliorate cellular damage in cell and Drosophila models of spinocerebellar ataxia type 3 through upregulation of Nrf2 pathway.
Dietary selenium protects adiponectin knockout mice against chronic inflammation induced colon cancer.
Glutathione-dependent and -independent oxidative stress-control mechanisms distinguish normal human mammary epithelial cell subsets.
Wu YL, Chang JC, Lin WY, Li CC, Hsieh M, Chen HW, Wang TS, Wu WT, Liu CS, Liu KL
Free radical biology & medicine 2018 Feb 1;115:309-317
Free radical biology & medicine 2018 Feb 1;115:309-317
Dietary selenium protects adiponectin knockout mice against chronic inflammation induced colon cancer.
Saxena A, Fayad R, Kaur K, Truman S, Greer J, Carson JA, Chanda A
Cancer biology & therapy 2017 Apr 3;18(4):257-267
Cancer biology & therapy 2017 Apr 3;18(4):257-267
Glutathione-dependent and -independent oxidative stress-control mechanisms distinguish normal human mammary epithelial cell subsets.
Kannan N, Nguyen LV, Makarem M, Dong Y, Shih K, Eirew P, Raouf A, Emerman JT, Eaves CJ
Proceedings of the National Academy of Sciences of the United States of America 2014 May 27;111(21):7789-94
Proceedings of the National Academy of Sciences of the United States of America 2014 May 27;111(21):7789-94
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HepG2 whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with GPX2 antibody [C1C3] (GTX100292) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: HepG2 (GTX27900) 12% SDS PAGE GTX100292 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HepG2 whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with GPX2 antibody [C1C3] (GTX100292) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of GPX2 protein from HepG2 whole cell extracts using 5 £gg of GPX2 antibody [C1C3] (GTX100292).Western blot analysis was performed using GPX2 antibody [C1C3] (GTX100292).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human gastric cancer, using GPX2(GTX100292) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded mouse muscle, using GPX2(GTX100292) antibody at 1:500 dilution.
