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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
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- Product number
- PA5-27150 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPX2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: HepG2. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.07 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Clinicopathological and prognostic significance of GPX2 protein expression in esophageal squamous cell carcinoma.
Lei Z, Tian D, Zhang C, Zhao S, Su M
BMC cancer 2016 Jul 7;16:410
BMC cancer 2016 Jul 7;16:410
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GPX2 Polyclonal Antibody detects GPX2 protein at granule by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GPX2 stained by GPX2 Polyclonal Antibody (Product # PA5-27150) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin Polyclonal Antibody [GT114] (Product # MA5-31466) diluted at 1:1,000. Blue: Fluoroshield with DAPI .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of GPX2 was performed in HepG2 whole cell extracts using 5 µg of GPX2 Polyclonal Antibody (Product # PA5-27150). Samples were transferred to a membrane and probed with GPX2 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
