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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Flow cytometry [3]
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Validation data
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- Product number
- TA502478 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502478, RRID:AB_11123788
- Product name
- RDH14 mouse monoclonal antibody, clone OTI1A7 (formerly 1A7)
- Antibody type
- Monoclonal
- Description
- RDH14 mouse monoclonal antibody, clone OTI1A7 (formerly 1A7)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- RDH14
- Isotype
- IgG
- Antibody clone number
- OTI1A7
- Vial size
- 100 µl
- Concentration
- 1.2 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RDH14 (RC203411, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RDH14.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RDH14 monoclonal antibody.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC203411 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RDH14 antibody(TA502478), and then analyzed by flow cytometry.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-RDH14 antibody(TA502478),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-RDH14 antibody(TA502478),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
