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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- LS-C167417 - Provider product page
- Provider
- LSBio
- Product name
- DIO2 Antibody (aa165-191) LS-C167417
- Antibody type
- Polyclonal
- Description
- Protein A purified
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Storage
- Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of lysates from human heart and human skeletal muscle tissue lysate (from left to right), using DIO2 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of DIO2 Antibody in MCF-7 cell line lysates (35 ug/lane). DIO2 (arrow) was detected using the purified antibody.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- DIO2 Antibody IHC of formalin-fixed and paraffin-embedded mouse brain followed by peroxidase-conjugated secondary antibody and DAB staining. This data demonstrates the use of the mouse DIO2 Antibody for immunohistochemistry.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- DIO2 Antibody IHC of formalin-fixed and paraffin-embedded mouse brain followed by peroxidase-conjugated secondary antibody and DAB staining. This data demonstrates the use of the mouse DIO2 Antibody for immunohistochemistry.
