F54462

antibody from NSJ Bioreagents

Targeting: DIO2 SelY, TXDI2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54462

Provider

NSJ Bioreagents

Product name

DIO2 Antibody / Type II Iodothyronine Deiodinase

Antibody type

Polyclonal

Description

This highly specific DIO2 antibody is suitable for use in Western blot/Flow cytometry/Immunohistochemistry applications with human and mouse samples.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the DIO2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) mouse heart and 2) human skeletal muscle lysate with DIO2 antibody. Predicted molecular weight ~31 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) heart and 2) skeletal muscle lysate with DIO2 antibody. Predicted molecular weight ~31 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human MCF7 cell lysate with DIO2 antibody. Predicted molecular weight ~31 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE mouse brain tissue with DIO2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human MCF7 cells with DIO2 antibody; Blue=isotype control, Green= DIO2 antibody.