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Immunoprecipitation
Other assayAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Other assay [1]
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- Product number
- A305-825A-T - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PPP1R21 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 10 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Detection of human PPP1R21 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PPP1R21 antibody A305-825A-M (lot A305-825A-M-1) used for IP at 15 µl per reaction. PPP1R21 was also immunoprecipitated by rabbit anti-PPP1R21 antibody A305-824A-M. For blotting immunoprecipitated PPP1R21, A305-824A-M was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.
