14132-1-AP

antibody from Proteintech Group

Targeting: GFPT1 GFA, GFAT, GFAT1, GFPT

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

14132-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#14132-1-AP, RRID:AB_2110155

Product name

GFPT1 antibody

Antibody type

Polyclonal

Description

KD/KO validated GFPT1 antibody (Cat. #14132-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

O-GlcNAc Modification Orchestrates HUWE1-Mediated Ubiquitination of TfR1 to Regulate Ferroptosis and Trophoblast Syncytialization in Preeclampsia.

Zhang H, Li M, Zhang J, Sun Q, Jin X, Liu R, Li X, Chen X, Wang A, Luo Z, Shang Y, Kong Y
Free radical biology & medicine 2026 Apr 8;

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Galactose treatment rescues neuromuscular junction transmission in glutamine-fructose-6-phosphate transaminase 1 (Gfpt1) deficient mice.

Holland SH, Carmona-Martinez R, O'Neil D, Ho K, O'Connor K, Azuma Y, Roos A, Spendiff S, Lochmüller H
Human molecular genetics 2025 Oct 14;34(21):1765-1779

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Improved VPS4B O-GlcNAc modification triggers lipid droplets transferring from adipocytes to nasopharyngeal carcinoma cells.

Yin H, Shan Y, Zhu Q, Yuan L, Ju F, Shi Y, Han Y, Wu R, Xia T, Zhang K, You Y, You B
Cancer & metabolism 2025 May 23;13(1):24

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MTHFD2 Enhances cMYC O-GlcNAcylation to Promote Sunitinib Resistance in Renal Cell Carcinoma.

Liu J, Huang G, Lin H, Yang R, Zhan W, Luo C, Wu Y, Chen L, Mao X, Chen J, Huang B
Cancer research 2025 Mar 14;85(6):1113-1129

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Protein O-GlcNAcylation and hexokinase mitochondrial dissociation drive heart failure with preserved ejection fraction.

Tatekoshi Y, Mahmoodzadeh A, Shapiro JS, Liu M, Bianco GM, Tatekoshi A, Camp SD, De Jesus A, Koleini N, De La Torre S, Wasserstrom JA, Dillmann WH, Thomson BR, Bedi KC, Margulies KB, Weinberg SE, Ardehali H
Cell metabolism 2025 Jul 1;37(7):1584-1600.e10

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Bioinformatics insights into the role of GFPT1 in breast invasive carcinoma: implications for tumor prognosis, immune modulation, and therapeutic applications.

Liang J, Deng X, Zhang Y, Fei T, Ouyang M, Yu C, Xiang Y, Jia D, Duan F
Frontiers in genetics 2024;15:1482929

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Compensatory activity of the PC-ME1 metabolic axis underlies differential sensitivity to mitochondrial complex I inhibition.

Del Prado L, Jaraíz-Rodríguez M, Agro M, Zamora-Dorta M, Azpiazu N, Calleja M, Lopez-Manzaneda M, de Juan-Sanz J, Fernández-Rodrigo A, Esteban JA, Girona M, Quintana A, Balsa E
Nature communications 2024 Oct 7;15(1):8682

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A Deficiency in Glutamine-Fructose-6-Phosphate Transaminase 1 (Gfpt1) in Skeletal Muscle Results in Reduced Glycosylation of the Delta Subunit of the Nicotinic Acetylcholine Receptor (AChRδ).

Holland SH, Carmona-Martinez R, O'Connor K, O'Neil D, Roos A, Spendiff S, Lochmüller H
Biomolecules 2024 Oct 3;14(10)

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O-GlcNAcylation modulates expression and abundance of N-glycosylation machinery in an inherited glycosylation disorder.

Matheny-Rabun C, Mokashi SS, Radenkovic S, Wiggins K, Dukes-Rimsky L, Angel P, Ghesquiere B, Kozicz T, Steet R, Morava E, Flanagan-Steet H
Cell reports 2024 Nov 26;43(11):114976

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O-GlcNAc Modification Is a Promising Therapeutic Target for Diabetic Retinopathy.

Dong W, Imdad L, Xu S, Wang Y, Liu C, Song S, Li Z, Kong Y, Kong L, Ren X
International journal of molecular sciences 2024 Jun 6;25(11)

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Enhancing GFPT1 expression with glutamine protects chondrocytes in osteoarthritis.

Zhang Z, Li X, Guo W, Huang Z
International immunopharmacology 2024 Dec 25;143(Pt 2):113427

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GFPT1 accelerates immune escape in breast cancer by modifying PD-L1 via O-glycosylation.

Tang W, Gao Y, Hong S, Wang S
BMC cancer 2024 Aug 29;24(1):1071

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Triamcinolone acetonide induces the autophagy of Ag85B-treated WI-38 cells via SIRT1/FOXO3 pathway.

Luo L, Zhou L, Luo L, Feng D, Ding Y, Lu Z, Nie G, Bai L, Xiao Y
Allergologia et immunopathologia 2023;51(2):27-35

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High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate.

Zhou P, Chang WY, Gong DA, Xia J, Chen W, Huang LY, Liu R, Liu Y, Chen C, Wang K, Tang N, Huang AL
Cell metabolism 2023 Nov 7;35(11):1961-1975.e6

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[WITHDRAWN] Hexokinase-1 mitochondrial dissociation and protein O-GlcNAcylation drive heart failure with preserved ejection fraction.

Tatekoshi Y, Shapiro JS, Liu M, Bianco GM, Tatekoshi A, De Jesus A, Koleini N, Wasserstrom JA, Dillmann WH, Weinberg SE, Ardehali H
Research square 2023 Jan 25;

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The UDPase ENTPD5 regulates ER stress-associated renal injury by mediating protein N-glycosylation.

Xu L, Zhou Y, Wang G, Bo L, Jin B, Dai L, Lu Q, Cai X, Hu L, Liu L, Wu Y, Chang X, Huang Y, Song L, Zhang T, Wang Y, Xiao Y, Zhang F, Liu L, Shi M, Wang T, Guo B
Cell death & disease 2023 Feb 27;14(2):166

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Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P.

Ai YL, Wang WJ, Liu FJ, Fang W, Chen HZ, Wu LZ, Hong X, Zhu Y, Zhang CX, Liu LY, Hong WB, Zhou B, Chen QT, Wu Q
Cell research 2023 Dec;33(12):904-922

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ER Stress-Perturbed Intracellular Protein O-GlcNAcylation Aggravates Podocyte Injury in Diabetes Nephropathy.

Song S, Hu T, Shi X, Jin Y, Liu S, Li X, Zou W, Wang C
International journal of molecular sciences 2023 Dec 18;24(24)

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GFPT1-Associated Congenital Myasthenic Syndrome Mimicking a Glycogen Storage Disease - Diagnostic Pitfalls in Myopathology Solved by Next-Generation-Sequencing.

Mensch A, Cordts I, Scholle L, Joshi PR, Kleeberg K, Emmer A, Beck-Woedl S, Park J, Haack TB, Stoltenburg-Didinger G, Zierz S, Deschauer M
Journal of neuromuscular diseases 2022;9(4):533-541

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GFPT1 promotes the proliferation of cervical cancer via regulating the ubiquitination and degradation of PTEN.

Li D, Guan M, Cao X, Zha ZQ, Zhang P, Xiang H, Zhou Y, Peng Q, Xu Z, Lu L, Liu G
Carcinogenesis 2022 Nov 23;43(10):969-979

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O-GlcNAc modification mediates aquaporin 3 to coordinate endometrial cell glycolysis and affects embryo implantation.

Zhang H, Qi J, Pei J, Zhang M, Shang Y, Li Z, Wang Y, Guo J, Sun K, Fan J, Sui L, Xu Y, Kong L, Kong Y
Journal of advanced research 2022 Mar;37:119-131

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A roadmap for the characterization of energy metabolism in human cardiomyocytes derived from induced pluripotent stem cells.

Emanuelli G, Zoccarato A, Reumiller CM, Papadopoulos A, Chong M, Rebs S, Betteridge K, Beretta M, Streckfuss-Bömeke K, Shah AM
Journal of molecular and cellular cardiology 2022 Mar;164:136-147

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GFAT1-linked TAB1 glutamylation sustains p38 MAPK activation and promotes lung cancer cell survival under glucose starvation.

Wei S, Zhao Q, Zheng K, Liu P, Sha N, Li Y, Ma C, Li J, Zhuo L, Liu G, Liang W, Jiang Y, Chen T, Zhong N
Cell discovery 2022 Aug 9;8(1):77

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DOT1L O-GlcNAcylation promotes its protein stability and MLL-fusion leukemia cell proliferation.

Song T, Zou Q, Yan Y, Lv S, Li N, Zhao X, Ma X, Liu H, Tang B, Sun L
Cell reports 2021 Sep 21;36(12):109739

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Tick extracellular vesicles enable arthropod feeding and promote distinct outcomes of bacterial infection.

Oliva Chávez AS, Wang X, Marnin L, Archer NK, Hammond HL, Carroll EEM, Shaw DK, Tully BG, Buskirk AD, Ford SL, Butler LR, Shahi P, Morozova K, Clement CC, Lawres L, Neal AJO, Mamoun CB, Mason KL, Hobbs BE, Scoles GA, Barry EM, Sonenshine DE, Pal U, Valenzuela JG, Sztein MB, Pasetti MF, Levin ML, Kotsyfakis M, Jay SM, Huntley JF, Miller LS, Santambrogio L, Pedra JHF
Nature communications 2021 Jun 17;12(1):3696

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Hypoxic acclimation improves cardiac redox homeostasis and protects heart against ischemia-reperfusion injury through upregulation of O-GlcNAcylation.

Ou W, Liang Y, Qin Y, Wu W, Xie M, Zhang Y, Zhang Y, Ji L, Yu H, Li T
Redox biology 2021 Jul;43:101994

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Insulin Resistance Does Not Impair Mechanical Overload-Stimulated Glucose Uptake, but Does Alter the Metabolic Fate of Glucose in Mouse Muscle.

Weyrauch LA, McMillin SL, Witczak CA
International journal of molecular sciences 2020 Jul 1;21(13)

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Mitochondrial Protein Poldip2 (Polymerase Delta Interacting Protein 2) Controls Vascular Smooth Muscle Differentiated Phenotype by O-Linked GlcNAc (N-Acetylglucosamine) Transferase-Dependent Inhibition of a Ubiquitin Proteasome System.

Paredes F, Williams HC, Quintana RA, San Martin A
Circulation research 2020 Jan 3;126(1):41-56

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Targeting tumor-intrinsic hexosamine biosynthesis sensitizes pancreatic cancer to anti-PD1 therapy.

Sharma NS, Gupta VK, Garrido VT, Hadad R, Durden BC, Kesh K, Giri B, Ferrantella A, Dudeja V, Saluja A, Banerjee S
The Journal of clinical investigation 2020 Jan 2;130(1):451-465

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Hydrogen sulfide accumulates LDL receptor precursor via downregulating PCSK9 in HepG2 cells.

Huang Y, Ning K, Li WW, Lin G, Hou CL, Wang MJ, Zhu YC
American journal of physiology. Cell physiology 2020 Dec 1;319(6):C1082-C1096

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Ginsenoside-Rb1 Improved Diabetic Cardiomyopathy through Regulating Calcium Signaling by Alleviating Protein O-GlcNAcylation.

Qin L, Wang J, Zhao R, Zhang X, Mei Y
Journal of agricultural and food chemistry 2019 Dec 26;67(51):14074-14085

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GFPT1 deficiency in muscle leads to myasthenia and myopathy in mice.

Issop Y, Hathazi D, Khan MM, Rudolf R, Weis J, Spendiff S, Slater CR, Roos A, Lochmüller H
Human molecular genetics 2018 Sep 15;27(18):3218-3232

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Consumption of a high fat diet promotes protein O-GlcNAcylation in mouse retina via NR4A1-dependent GFAT2 expression.

Dai W, Dierschke SK, Toro AL, Dennis MD
Biochimica et biophysica acta. Molecular basis of disease 2018 Dec;1864(12):3568-3576

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MPI depletion enhances O-GlcNAcylation of p53 and suppresses the Warburg effect.

Shtraizent N, DeRossi C, Nayar S, Sachidanandam R, Katz LS, Prince A, Koh AP, Vincek A, Hadas Y, Hoshida Y, Scott DK, Eliyahu E, Freeze HH, Sadler KC, Chu J
eLife 2017 Jun 23;6

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A Genetic Model to Study Increased Hexosamine Biosynthetic Flux.

Hugo SE, Schlegel A
Endocrinology 2017 Aug 1;158(8):2420-2426

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Hexosamine biosynthesis in keratinocytes: roles of GFAT and GNPDA enzymes in the maintenance of UDP-GlcNAc content and hyaluronan synthesis.

Oikari S, Makkonen K, Deen AJ, Tyni I, Kärnä R, Tammi RH, Tammi MI
Glycobiology 2016 Jul;26(7):710-22

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UDP-sugar substrates of HAS3 regulate its O-GlcNAcylation, intracellular traffic, extracellular shedding and correlate with melanoma progression.

Deen AJ, Arasu UT, Pasonen-Seppänen S, Hassinen A, Takabe P, Wojciechowski S, Kärnä R, Rilla K, Kellokumpu S, Tammi R, Tammi M, Oikari S
Cellular and molecular life sciences : CMLS 2016 Aug;73(16):3183-204

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A 3'-UTR mutation creates a microRNA target site in the GFPT1 gene of patients with congenital myasthenic syndrome.

Dusl M, Senderek J, Müller JS, Vogel JG, Pertl A, Stucka R, Lochmüller H, David R, Abicht A
Human molecular genetics 2015 Jun 15;24(12):3418-26

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Trivalent chromium inhibits TSP-1 expression, proliferation, and O-GlcNAc signaling in vascular smooth muscle cells in response to high glucose in vitro.

Ganguly R, Sahu S, Chavez RJ, Raman P
American journal of physiology. Cell physiology 2015 Jan 15;308(2):C111-22

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Hexosamine biosynthetic pathway mutations cause neuromuscular transmission defect.

Senderek J, Müller JS, Dusl M, Strom TM, Guergueltcheva V, Diepolder I, Laval SH, Maxwell S, Cossins J, Krause S, Muelas N, Vilchez JJ, Colomer J, Mallebrera CJ, Nascimento A, Nafissi S, Kariminejad A, Nilipour Y, Bozorgmehr B, Najmabadi H, Rodolico C, Sieb JP, Steinlein OK, Schlotter B, Schoser B, Kirschner J, Herrmann R, Voit T, Oldfors A, Lindbergh C, Urtizberea A, von der Hagen M, Hübner A, Palace J, Bushby K, Straub V, Beeson D, Abicht A, Lochmüller H
American journal of human genetics 2011 Feb 11;88(2):162-72

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 14132-1-AP(GFPT1 antibody) at dilution of 1:500

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using GFPT1 antibody 14132-1-AP at 1:100 dilution and PE-labeled goat anti-rabbit IgG(red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human gliomas using 14132-1-AP(GFPT1 antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human gliomas using 14132-1-AP(GFPT1 antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue