MA5-31801

antibody from Invitrogen Antibodies

Targeting: MOB1A C2orf6, FLJ10788, FLJ11595, Mats1, MOB1, Mob4B, MOBK1B, MOBKL1B

Western blot (Data presented on provider website)

ELISA (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-31801

Provider

Invitrogen Antibodies

Product name

MOB1A Monoclonal Antibody (3E7B2)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-31801 has been tested in indirect ELISA.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

3E7B2

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Thrombospondin-1 Plays an Essential Role in Yes-Associated Protein Nuclear Translocation during the Early Phase of Trypanosoma cruzi Infection in Heart Endothelial Cells.

Arun A, Rayford KJ, Cooley A, Rachakonda G, Villalta F, Pratap S, Lima MF, Sheibani N, Nde PN
International journal of molecular sciences 2020 Jul 12;21(14)

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ELISA analysis of MOB1 in Control Antigen (black line, 100 ng); Antigen (purple line, 10 ng); Antigen (blue line, 50 ng); Antigen (red line, 100 ng). Samples were incubated with MOB1 monoclonal antibody (Product # MA5-31801) using a dilution of 1:10,000.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of MOB1 in HeLa cells (green). Sample was incubated with MOB1 monoclonal antibody (Product # MA5-31801) using a dilution of 1:200-1:400 followed by negative control (red).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 TSP-1 is essential for activation of hippo signaling cascade during the early phase of T. cruzi infection. Lysates (20 µg) from WT or TSP-1KO MHEC challenged with T . cruzi at different time points were resolved by SDS-PAGE, blotted, and probed with antibodies against ( A ) SAV1, ( B ) MOB1A in WT MHEC and ( C ) SAV1, ( D ) MOB1A in TSP-1KO MHEC, and developed as described. The blots were stripped, reprobed with antibodies against housekeeping GAPDH and developed with the corresponding IRDye conjugated secondary antibody. The developed blots were scanned using the infrared fluorescence detection Odyssey Imaging Systems. The normalized fold change in the level of each unphosphorylated protein was determined and plotted in the bar graph for WT MHEC ( A , lower panel) SAV1, ( B , lower panel) MOB1A, respectively and for TSP-1KO MHEC ( C , lower panel) SAV1 and ( D , lower panel) MOB1A, respectively. The bar graphs represent mean values +- SE from three independent biological replicates. The value of p < 0.05 was considered significant. * p < 0.05; ** p < 0.01; *** p < 0.001.