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LearnNB100-174
antibody from Novus Biologicals
Targeting: THOC1
HPR1, P84
Western blot
Immunocytochemistry Immunoprecipitation Immunohistochemistry Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [10]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- NB100-174 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-174, RRID:AB_2202241
- Product name
- Mouse Monoclonal hHpr1-p84-Thoc1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. This is specific for p84.
- Reactivity
- Human, Mouse, Rat, Hamster, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references The THO Complex Coordinates Transcripts for Synapse Development and Dopamine Neuron Survival.
SMYD3 Promotes Homologous Recombination via Regulation of H3K4-mediated Gene Expression.
Cellular protein HAX1 interacts with the influenza A virus PA polymerase subunit and impedes its nuclear translocation.
Integrative analysis of the ubiquitin proteome isolated using Tandem Ubiquitin Binding Entities (TUBEs).
Krüppel-like transcription factor 4 contributes to maintenance of telomerase activity in stem cells.
Linking transcriptional elongation and messenger RNA export to metastatic breast cancers.
Characterization of the mouse JAB1 cDNA and protein.
Characterization of the mouse JAB1 cDNA and protein.
Maeder CI, Kim JI, Liang X, Kaganovsky K, Shen A, Li Q, Li Z, Wang S, Xu XZS, Li JB, Xiang YK, Ding JB, Shen K
Cell 2018 Sep 6;174(6):1436-1449.e20
Cell 2018 Sep 6;174(6):1436-1449.e20
SMYD3 Promotes Homologous Recombination via Regulation of H3K4-mediated Gene Expression.
Chen YJ, Tsai CH, Wang PY, Teng SC
Scientific reports 2017 Jun 19;7(1):3842
Scientific reports 2017 Jun 19;7(1):3842
Cellular protein HAX1 interacts with the influenza A virus PA polymerase subunit and impedes its nuclear translocation.
Hsu WB, Shih JL, Shih JR, Du JL, Teng SC, Huang LM, Wang WB
Journal of virology 2013 Jan;87(1):110-23
Journal of virology 2013 Jan;87(1):110-23
Integrative analysis of the ubiquitin proteome isolated using Tandem Ubiquitin Binding Entities (TUBEs).
Lopitz-Otsoa F, Rodriguez-Suarez E, Aillet F, Casado-Vela J, Lang V, Matthiesen R, Elortza F, Rodriguez MS
Journal of proteomics 2012 Jun 6;75(10):2998-3014
Journal of proteomics 2012 Jun 6;75(10):2998-3014
Krüppel-like transcription factor 4 contributes to maintenance of telomerase activity in stem cells.
Wong CW, Hou PS, Tseng SF, Chien CL, Wu KJ, Chen HF, Ho HN, Kyo S, Teng SC
Stem cells (Dayton, Ohio) 2010 Sep;28(9):1510-7
Stem cells (Dayton, Ohio) 2010 Sep;28(9):1510-7
Linking transcriptional elongation and messenger RNA export to metastatic breast cancers.
Guo S, Hakimi MA, Baillat D, Chen X, Farber MJ, Klein-Szanto AJ, Cooch NS, Godwin AK, Shiekhattar R
Cancer research 2005 Apr 15;65(8):3011-6
Cancer research 2005 Apr 15;65(8):3011-6
Characterization of the mouse JAB1 cDNA and protein.
Bounpheng MA, Melnikova IN, Dodds SG, Chen H, Copeland NG, Gilbert DJ, Jenkins NA, Christy BA
Gene 2000 Jan 25;242(1-2):41-50
Gene 2000 Jan 25;242(1-2):41-50
Characterization of the mouse JAB1 cDNA and protein.
Bounpheng MA, Melnikova IN, Dodds SG, Chen H, Copeland NG, Gilbert DJ, Jenkins NA, Christy BA
Gene 2000 Jan 25;242(1-2):41-50
Gene 2000 Jan 25;242(1-2):41-50
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (30 ug of whole cell lysate) A: 293T B: NIH-3T3 C: mouse brain D: PC-12 7. 5% SDS PAGE; antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (50 ug of whole cell lysate) A: mouse Cerebellum 7. 5% SDS PAGE; antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (30 ug of cell lysate) A: HeLa B: HeLa cytosol fraction C: HeLa nucleus fraction 7. 5% SDS PAGE; antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (30 ug of whole cell lysate) A: HeLa B: HeLa nucleus 7. 5% SDS PAGE; antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug 7. 5% SDS PAGE; antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: 293T D: A431 E: HeLa F: HepG2 G: H1299 H: HCT116 I: MCF-7 J: NT2D1 K: PC-3 L: U87-MG 7. 5% SDS PAGE; antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Mouse Cerebellum, whole cell lysate (50 ug), stained with hHpr1-p84-Thoc1 Antibody (5E10), diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - p84 western in 293T cells. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Lane 1: total lysate, Lane 2: cytoplasmic fraction, Lane 3: membrane fraction, Lane 4: nuclear fraction.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - p84 antibody [5E10] detects p84 protein by western blot analysis. A. 30 ug 293T whole cell lysate/extract. B. 30 ug A431 whole cell lysate/extract. C. 30 ug HeLa whole cell lysate/extract. D. 30 ug HepG2 whole cell lysate/extract. E. 30 ug A375 whole cell lysate/extract. 7.5% SDS-PAGE. p84 antibody [5E10] dilution: 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - hHpr1-p84-Thoc1 antibody [5E10] immunoprecipitates p84 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of p84 protein expressing HepG2 cells B : Control with 3 ug of pre-immune mouse IgG C : Immunoprecipitation of p84 by 3 ug of p84 antibody [5E10] (NB100-174) 7.5% SDS-PAGE The immunoprecipitated p84 protein was detected by p84 antibody [5E10] (NB100-174). EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - hHpr1-p84-Thoc1 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Nuclear Matrix Protein p84 stained by hHpr1-p84-Thoc1 antibody [5E10] (NB100-174). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Nuclear Matrix Protein p84 stained by Nuclear Matrix Protein p84 antibody [5E10] diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
