PA5-27816

antibody from Invitrogen Antibodies

Targeting: THOC1 HPR1, P84

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27816

Provider

Invitrogen Antibodies

Product name

Nuclear Matrix Protein p84 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, HeLa, HepG2, PC-12, Rat-2, THOC1 shRNA-transfected 293T. Predicted reactivity: Mouse (96%), Rat (96%), Chicken (89%), Rhesus Monkey (100%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat, Chicken/Avian

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

ITGBL1 promotes EMT, invasion and migration by activating NF-κB signaling pathway in prostate cancer.

Li W, Li S, Yang J, Cui C, Yu M, Zhang Y
OncoTargets and therapy 2019;12:3753-3763

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Downregulation of miR‑505‑3p predicts poor bone metastasis‑free survival in prostate cancer.

Tang Y, Wu B, Huang S, Peng X, Li X, Huang X, Zhou W, Xie P, He P
Oncology reports 2019 Jan;41(1):57-66

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N-Myc promotes therapeutic resistance development of neuroendocrine prostate cancer by differentially regulating miR-421/ATM pathway.

Yin Y, Xu L, Chang Y, Zeng T, Chen X, Wang A, Groth J, Foo WC, Liang C, Hu H, Huang J
Molecular cancer 2019 Jan 18;18(1):11

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Downregulation of miR‑19a‑3p promotes invasion, migration and bone metastasis via activating TGF‑β signaling in prostate cancer.

Wa Q, Li L, Lin H, Peng X, Ren D, Huang Y, He P, Huang S
Oncology reports 2018 Jan;39(1):81-90

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Oncogenic miR-210-3p promotes prostate cancer cell EMT and bone metastasis via NF-κB signaling pathway.

Ren D, Yang Q, Dai Y, Guo W, Du H, Song L, Peng X
Molecular cancer 2017 Jul 10;16(1):117

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Downregulation of miR-141-3p promotes bone metastasis via activating NF-κB signaling in prostate cancer.

Huang S, Wa Q, Pan J, Peng X, Ren D, Huang Y, Chen X, Tang Y
Journal of experimental & clinical cancer research : CR 2017 Dec 4;36(1):173

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Nuclear Matrix Protein p84 using 30 µg of A549 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Nuclear Matrix Protein p84 polyclonal antibody (Product # PA5-27816) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Nuclear Matrix Protein p84 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Nuclear Matrix Protein p84 Polyclonal Antibody (Product # PA5-27816) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Nuclear Matrix Protein p84 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Nuclear Matrix Protein p84 Polyclonal Antibody (Product # PA5-27816) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Nuclear Matrix Protein p84 Polyclonal Antibody detects Nuclear Matrix Protein p84 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nuclear Matrix Protein p84 protein stained by Nuclear Matrix Protein p84 Polyclonal Antibody (Product # PA5-27816) diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Nuclear Matrix Protein p84 (Product # PA5-27816) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Cross-linked ChIP was performed with HeLa chromatin extract and 5 µg of either control rabbit IgG or Nuclear Matrix Protein p84 Polyclonal Antibody (Product # PA5-27816). The precipitated DNA was detected by PCR with primer set targeting to calreticulin.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Nuclear Matrix Protein p84 Polyclonal Antibody immunoprecipitates p84 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 µg whole cell lysate/extract of p84 protein expressing HepG2 cells B : Control with 3 µg of pre-immune rabbit IgG C : Immunoprecipitation of p84 by 3 µg of Nuclear Matrix Protein p84 Polyclonal Antibody (Product # PA5-27816) 7.5% SDS-PAGE The immunoprecipitated p84 protein was detected by Nuclear Matrix Protein p84 Polyclonal Antibody (Product # PA5-27816) diluted at 1:1,000. Anti-rabbit IgG (HRP) was used as a secondary reagent.