TA339403

antibody from OriGene

Targeting: SEMA4F SEMAM, SEMAW

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

TA339403

Provider

OriGene

Product name

Rabbit Polyclonal Anti-SEMA4F Antibody

Antibody type

Polyclonal

Description

Rabbit Polyclonal Anti-SEMA4F Antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

SEMA4F

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µl

Concentration

1mg/mL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

human cell line A431; Primary antibody dilution and incubation time: 1: 600, 4 degree overnight.Secondary antibody used and dilution and incubation time: 1: 3000, RT 2 hours

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

WB Suggested Anti-SEMA4F Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Transfected 293T

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Lanes: 1. Untransfected MIA-PACA2 cell lysate; 2. Untransfected MIA-PACA2 cell lysate; 3. Untransfected MIA-PACA2 cell lysate; 4. hSEMA4F transfected MIA-PACA2 cell lysate; 5. Untransfected MIA-PACA2 cell lysate; 6. hSEMA4F transfected MIA-PACA2 cell lysa

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Lanes: 1. A431 cell lysate + control siRNA 2. A431 cell lysate + SEMA4F siRNA; Primary Antibody Dilution: 1: 600; Secondary Antibody: Donkey Anti-rabbit HRP; Secondary Antibody Dilution: 1: 3000; Gene Name: SEMA4F; Submitted by: Dr. Tianliang Sun, Max Pla

Validation comment

WB