Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
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Validation data
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- Product number
- PA5-42878 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SEMA4F Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: PFSGERPRRI DWMVPEAHRQ NCRKKGKKEG DLGGRKTLQQ RWTTFLKADL
- Concentration
- 0.5 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human A431 cell lysate using an anti-SEMA4F polyclonal antibody (Product # PA5-42878). Primary Antibody Dilution: 1:600; Secondary Antibody: Donkey Anti-rabbit HRP; Secondary Antibody Dilution: 1:3000. LANE 1)A431 cell lysate + control siRNA; LANE 2) A431 cell lysate + SEMA4F siRNA.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human A431 cells using an anti-SEMA4F polyclonal antibody (Product # PA5-42878).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human MIA-PACA2 cell lysate using an anti-SEMA4F polyclonal antibody (Product # PA5-42878). Primary Antibody Dilution: 1:600; Secondary Antibody: Donkey Anti-rabbit HRP; Secondary Antibody Dilution: 1:3000. LANE 1)Untransfected MIA-PACA2 cell lysate; LANE 2) Untransfected MIA-PACA2 cell lysate; LANE 3) Untransfected MIA-PACA2 cell lysate; LANE 4) hSEMA4F transfected MIA-PACA2 cell lysate; LANE 5) Untransfected MIA-PACA2 cell lysate; LANE 6) hSEMA4F transfected MIA-PACA2 cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of transfected 293T cells using an anti-SEMA4F polyclonal antibody (Product # PA5-42878).