PA5-87894

antibody from Invitrogen Antibodies

Targeting: SEMA4F SEMAM, SEMAW

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-87894

Provider

Invitrogen Antibodies

Product name

SEMA4F Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: RKTLQQRWTT FLKADLLCPG PEHGRASSVL QDVAVLRPEL GAGTPIFYGI FSSQWEGATI SAVCAFRPQD IRTVLNGPFR ELKHDCNRGL PVVDNDVPQP RPGECITNNM KLRHFGSSLS LPDRVLTFIR DHPLMDRPVF P; Positive Samples: SH-SY5Y, HepG2, A-549, Mouse brain, Mouse liver, Mouse lung, Rat brain, Rat liver; Cellular Location: Membrane, Single-pass type I membrane protein

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

3.3 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SEMA4F in paraffin-embedded human esophageal cancer. Samples were incubated with SEMA4F Polyclonal antibody (Product # PA5-87894) using a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.