PA5-22006

antibody from Invitrogen Antibodies

Targeting: SUCLG1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-22006

Provider

Invitrogen Antibodies

Product name

SUCLG1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Recommended positive controls: A431, mouse brain, rat brain. Predicted reactivity: Mouse (93%), Rat (90%), Zebrafish (83%), Pig (86%), Chicken (87%), Bovine (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SUCLG1 using 30 µg of A431 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a SUCLG1 polyclonal antibody (Product # PA5-22006) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using SUCLG1 Polyclonal Antibody (Product # PA5-22006). Sample (20 µg of whole cell lysate). Lane A: mouse brain. 10% SDS PAGE. SUCLG1 Polyclonal Antibody (Product # PA5-22006) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using SUCLG1 Polyclonal Antibody (Product # PA5-22006). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SUCLG1 Polyclonal Antibody (Product # PA5-22006) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SUCLG1 Polyclonal Antibody detects SUCLG1 protein by western blot analysis. A.50 µg rat brain lysate/extract.10% SDS-PAGE. SUCLG1 Polyclonal Antibody (Product # PA5-22006) dilution: 1:3,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SUCLG1 showing staining in the mitochondria of HeLa cells. HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min and stained using a SUCLG1 polyclonal antibody (Product # PA5-22006) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SUCLG1 Polyclonal Antibody detects SUCLG1 protein at mitochondria by confocal immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min. Green: SUCLG1 protein stained by SUCLG1 Polyclonal Antibody (Product # PA5-22006) diluted at 1:500. Blue: Hoechst 33342 staining. [Images captured by Olympus FV10i Confocal Laser Scanning Microscope].

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded N87 xenograft, using SUCLG1 (Product # PA5-22006) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.