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LearnA304-609A
antibody from Invitrogen Antibodies
Targeting: IWS1
DKFZp761G0123, FLJ10006, FLJ14655, FLJ32319
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Other assay [1]
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- Product number
- A304-609A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IWS1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human IWS1 by western blot. Samples: Whole cell lysate (10 µg) from Jurkat, Hep-G2, K-562, MCF-7, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-IWS1 antibody (Product # A304-609A lot 2) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human IWS1 by western blot. Samples: Whole cell lysate (10 µg) from Jurkat, Hep-G2, K-562, MCF-7, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-IWS1 antibody (Product # A304-609A lot 2) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse IWS1 by western blot. Samples: Whole cell lysate (10 µg) from CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-IWS1 antibody ((Product # A304-609A lot 2) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human IWS1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-IWS1 antibody A304-609A (lot A304-609A-1) used for IP at 6 µg per reaction. IWS1 was also immunoprecipitated by rabbit anti-IWS1 antibody BL17595. For blotting immunoprecipitated IWS1, A304-609A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse IWS1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-IWS1 antibody A304-609A (lot A304-609A-1) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human IWS1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-IWS1 antibody (Product # A304-609A; Lot 2) used for IP at 6 µg per reaction. IWS1 was also immunoprecipitated by a previous lot of this antibody (Product # A304-609A; Lot 1) and a second antibody against a different epitope of IWS1 (BL17594). For blotting immunoprecipitated IWS1, Product # A304-609A was used at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.
