LS-C357462

antibody from LSBio

Targeting: DMD BMD, DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272, MRX85

Western blot (Enhanced validation)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C357462

Provider

LSBio

Product name

DMD / Dystrophin Antibody (aa3076-3404) LS-C357462

Antibody type

Polyclonal

Description

Immunogen affinity purified

Reactivity

Human, Mouse, Rat

Host

Rabbit

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Dystrophin antibody Western blot. All lanes: Anti Dystrophin at 0.5 ug/ml. Lane 1: SMMC Whole Cell Lysate at 40 ug. Lane 2: HEPA Whole Cell Lysate at 40 ug. Predicted band size: 427 kD. Observed band size: 427 kD.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of Dystrophin using anti-Dystrophin antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: mouse HEPA1-6 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dystrophin antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Dystrophin at approximately 427KD. The expected band size for Dystrophin is at 427KD.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Dystrophin antibody IHC-paraffin: Human Lung Cancer Tissue.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Dystrophin antibody IHC-paraffin: Mouse Brain Tissue.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Dystrophin antibody IHC-paraffin: Rat Cardiac Muscle Tissue.