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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- MA5-45458 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SHANK3 Monoclonal Antibody (S69), APC
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- S69
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Rat brain membrane lysate showing detection of SHANK3 protein. Load: 15 µg. Blocking: 1.5% BSA for 30 minutes at RT. Samples were incubated with SHANK3 monoclonal antibody (Product # MA5-45458) at 1:1,000 for 2 hours at RT, followed by Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% PFA for 15 min. Samples were incubated with SHANK3 monoclonal antibody (Product # MA5-45458) at 1:50 for overnight at 4°C with slow rocking, followed by AlexaFluor 488 at 1:1,000 for 1 hour at RT. Counterstain used was Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SHANK3 antibody (D) Composite.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis using HaCaT cells. Fixation involved Cold 100% methanol for 10 minutes at -20°C. Samples were incubated with SHANK3 monoclonal antibody (Product # MA5-45458) at 1:100 for 1 hour at RT, followed by FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Borderline positive.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis using backskin. Fixation involved Bouins Fixative and paraffin-embedded. Samples were incubated with SHANK3 monoclonal antibody (Product # MA5-45458) at 1:100 for 1 hour at RT, followed by FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Early stages of filaggrin-like and dermal staining.
