PAB27521

antibody from Abnova Corporation

Targeting: ZNF674 MRX92, ZNF673B

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB27521

Provider

Abnova Corporation

Product name

ZNF674 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant ZNF674.

Antigen sequence

IKHQRTHTGEKPFVCDKCPKAFKSSYHLIRHEKTH
IRQAFYKGIKCTTSSLIYQRIHTSEKPQCSEHGKA
SDEKPSPT


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with ZNF674 polyclonal antibody (Cat # PAB27521) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG with ZNF674 polyclonal antibody (Cat # PAB27521) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human testis with ZNF674 polyclonal antibody (Cat # PAB27521) shows distinct positivity in spermatozoa at 1:20-1:50 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)