HPA038068

antibody from Atlas Antibodies

Targeting: SH3BP5L KIAA1720

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

HPA038068

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA038068, RRID:AB_10670651

Product name

Anti-SH3BP5L

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human SH3BP5L, Gene description: SH3-binding domain protein 5-like, Alternative Gene Names: KIAA1720, Validated applications: WB, IHC, ICC, Uniprot ID: Q7L8J4, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.2 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

Proximity labelling identifies pro-migratory endocytic recycling cargo and machinery of the Rab4 and Rab11 families

Wilson B, Flett C, Gemperle J, Lawless C, Hartshorn M, Hinde E, Harrison T, Chastney M, Taylor S, Allen J, Norman J, Zacharchenko T, Caswell P
Journal of Cell Science 2023;136(12)

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and SH3BP5L over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY410767).

Sample type

Human

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, the Golgi apparatus & vesicles.

Sample type

Human