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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [2]
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Validation data
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- Product number
- 24034-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ANKRD27 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.29 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF-7 cells were subjected to SDS PAGE followed by western blot with 24034-1-AP ( ANKRD27 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed MCF-7 cells using 24034-1-AP (ANKRD27 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 24034-1-AP (ANKRD27 Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 24034-1-AP ( ANKRD27 Antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-ANKRD27 (IP:24034-1-AP, 4ug; Detection:24034-1-AP 1:1000) with A431 cells lysate 2000ug.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-ANKRD27 (IP:24034-1-AP, 4 ug; Detection:24034-1-AP 1:500) with MCF-7 cells lysate 3200 ug.
