PA5-59595

antibody from Invitrogen Antibodies

Targeting: LEUTX

Provider product page for PA5-59595

Immunocytochemistry

Immunohistochemistry

Other assay

Antibody data

Antibody Data
Antigen structure
References [1]
Comments [0]
Validations
Immunocytochemistry [1]
Other assay [2]

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Product number: PA5-59595 - Provider product page
Provider: Invitrogen Antibodies
Product name: LEUTX Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Immunogen sequence: AKWKRQQRQQ MQTRPSLGPA NQTTSVKKEE TPSAITTANI RPVSPGISDA NDHDLREPSG IKNPGGASAS ARVSSW Highest antigen sequence identity to the following orthologs: Mouse - 30%, Rat - 29%.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

LEUTX protein structure - PA5-59595 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: 3 Figure Comparison with DUX4 target gene expression. (a) RNAScope staining of DUX4 target genes LEUTX , KDM4E , SLC34A2 , and ZSCAN4 . Merged images with DAPI (blue) staining are also shown, as indicated at the top. Unlike DUX4 , staining of target genes is spread along the myotube and in the cytoplasm. Approximately 2% of myotubes showed a positive RNAScope target gene signal (right). Scale bar = 20 mum. (b) Costaining of LEUTX RNAScope (green) and LEUTX protein immunofluorescence staining (red) in control and FSHD myotubes at Day 6 of differentiation. Merged images with DAPI (blue) staining are also shown, as indicated at the top. The middle panel shows a typical example of RNA and protein colocalization, whereas the bottom panel shows an example of weak protein staining without RNA. Numbers of myotubes with corresponding patterns in the total myotubes counted are shown in parentheses. Percentages of myotubes with LEUTX RNA only, LEUTX RNA and protein, or LEUTX protein only are shown on the right. Scale bar = 20 mum. DAPI, 4',6-diamidino-2-phenylindole; FSHD, facioscapulohumeral dystrophy

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: 5 Figure KDM4E expression is regulated by DUX4 target transcription factors. (a) Individual time-course RNAScope analysis of DUX4, LEUTX , and KDM4E in FSHD2 myotubes for the indicated days of differentiation in Figure 4b. These include all the positive myotubes for each transcript. (b) Comparison of correlation between KDM4E and DUX4/LEUTX expression based on the FSHD2 RNAScope data in Figure 4b. The high phi values indicate a strong positive relationship between KDM4E and LEUTX RNA expression for the entire duration of the time course, with a perfect positive correlation ( phi = 1) on Day 4. Then, the phi values decrease but are more significant than those between KDM4E and DUX4 expression until Day 13. p Values (unpaired, two-tailed t test) are for the correlation differences of indicated genes on the same day. (c) Individual time-course RNAScope analysis of DUX4, LEUTX , and KDM4E in FSHD1 myotubes for the indicated days of differentiation in Figure 4f. These include all the positive myotubes for each transcript. (d) Comparison of correlation between KDM4E and DUX4/LEUTX expression based on the FSHD1 RNAScope data in Figure 4f. The results indicate a strong positive relationship between KDM4E and LEUTX RNA expression for the entire duration of the time course. The phi values between them are significantly higher than those between KDM4E and DUX4 expression. p Values (unpaired, two-tailed t test) are for the correlation differences of indicated genes on the same day. (e) T