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Western blot
ELISA
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- PA5-49695 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NFKBID Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The antibody detects endogenous level of total NFKBID protein.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.9 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of NFKBID was performed by loading Lane1: Hela cell lysate (40µg), Lane2: 231 cell lysate (40µg) on to a 12%+10% SDS-PAGE gel. Proteins were transferred to a membrane and the membrane was probed with a NFKBID antibody (Product # PA5-49695) at a 1/450 dilution for 2 minutes, followed by Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution.
