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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [5]
- Other assay [1]
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- Product number
- PA5-62972 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Spectrin beta-4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: AWEERFSSLR RLTTIEKIKA EQSKQPPTPL LGRKFFGDPT ELAAKAAPLL RPGGYERGLE PLARRASDTL SAEVRTRVGY VRQELKPERL QPRIDRLP Highest antigen sequence identity to the following orthologs: Mouse - 98%, Rat - 98%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Plug-and-Play Protein Modification Using Homology-Independent Universal Genome Engineering.
Gao Y, Hisey E, Bradshaw TWA, Erata E, Brown WE, Courtland JL, Uezu A, Xiang Y, Diao Y, Soderling SH
Neuron 2019 Aug 21;103(4):583-597.e8
Neuron 2019 Aug 21;103(4):583-597.e8
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of Spectrin beta-4 in human cell line U-2 OS using a Spectrin beta-4 Polyclonal Antibody (Product # PA5-62972) shows localization to nucleus, nucleoli and cytosol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Spectrin beta-4 in human cerebral cortex and skin tissues using Spectrin beta-4 Polyclonal Antibody (Product # PA5-62972). Corresponding SPTBN4 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Spectrin beta-4 in human cerebral cortex using Spectrin beta-4 Polyclonal Antibody (Product # PA5-62972) shows weak to moderate cytoplasmic positivity in neurons.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Spectrin beta-4 in human pancreas using Spectrin beta-4 Polyclonal Antibody (Product # PA5-62972) shows no positivity in exocrine glandular cells as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Spectrin beta-4 in human skin using Spectrin beta-4 Polyclonal Antibody (Product # PA5-62972) shows no positivity in keratinocytes as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Spectrin beta-4 in human colon using Spectrin beta-4 Polyclonal Antibody (Product # PA5-62972) shows no positivity in glandular cells as expected.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
