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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 12973-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CDKL5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Aliquoting is unnecessary for -20°C storage.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 387 µg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 12973-1-AP (CDKL5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12973-1-AP (CDKL5 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-CDKL5 (IP:12973-1-AP, 4 µg; Detection:12973-1-AP 1:300) with mouse brain tissue lysate 3200 µg.
