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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [5]
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- Product number
- PA5-90694 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TENM1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: QPVEGELYAN GVSKGNRGTE SMDTTYSPIG GKVSDKSEKK VFQKGRAIDT GEVDIGAQVM QTIPPGLFWR FQITIHHPIY LKFNISLAKD SLLGIYGRRN IPPTHTQFDF VKLMDGKQLV KQDSKGSDDT QHSPRNLILT SLQETGFIEY MDQGPWYLAF YNDGKKMEQV FVLTTAIEIM DDCSTNCNGN G; Positive Samples: U-87MG, U-251MG, HeLa; Cellular Location: Cell membrane, Cytoplasm, Nucleus, Nucleus matrix, Nucleus speckle, Single-pass membrane protein, cytoskeleton
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.25 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TENM1 in extracts of various cell lines. Samples were incubated with TENM1 Polyclonal antibody (Product # PA5-90694) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of TENM1 in C6 cells. Samples were incubated with TENM1 Polyclonal antibody (Product # PA5-90694) using a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of TENM1 was performed in L929 cells using TENM1 Polyclonal Antibody (Product # PA5-90694) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of TENM1 was performed in U-2 OS cells using TENM1 Polyclonal Antibody (Product # PA5-90694) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of TENM1 in L929 cells. Samples were incubated with TENM1 Polyclonal antibody (Product # PA5-90694) using a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of TENM1 in U-2 OS cells. Samples were incubated with TENM1 Polyclonal antibody (Product # PA5-90694) using a dilution of 1:100. Blue: DAPI for nuclear staining.
