PA5-57826

antibody from Invitrogen Antibodies

Targeting: DNAH1 DNAHC1, HDHC7, HL-11, HL11, XLHSRF-1

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-57826

Provider

Invitrogen Antibodies

Product name

DNAH1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: RIVKVMDDYQ VMDEFLYNLS SDDFNDKWIA SNWPSKILGQ IELVQQQHVE DEEKFRKIQI MDQNNFQEKL EGLQLVVAGF S Highest antigen sequence identity to the following orthologs: Mouse - 86%, Rat - 85%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.3 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Genetic Defects in DNAH2 Underlie Male Infertility With Multiple Morphological Abnormalities of the Sperm Flagella in Humans and Mice.

Hwang JY, Nawaz S, Choi J, Wang H, Hussain S, Nawaz M, Lopez-Giraldez F, Jeong K, Dong W, Oh JN, Bilguvar K, Mane S, Lee CK, Bystroff C, Lifton RP, Ahmad W, Chung JJ
Frontiers in cell and developmental biology 2021;9:662903

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of DNAH1 in human fallopian tube using a DNAH1 Polyclonal Antibody (Product # PA5-57826) shows membranous positivity in glandular cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of DNAH1 in human liver using DNAH1 Polyclonal Antibody (Product # PA5-57826) shows strong cytoplasmic granular positivity in hepatocytes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of DNAH1 in human duodenum using DNAH1 Polyclonal Antibody (Product # PA5-57826) shows cytoplasmic-membranous positivity in glandular cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of DNAH1 in human cerebral cortex using DNAH1 Polyclonal Antibody (Product # PA5-57826) shows moderate cytoplasmic positivity in neuropil.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIGURE 3 Flagellar ultrastructure is disorganized, and axonemal proteins express abnormally in Dnah2 - sperm. (A,B) Scanning electron microscopy images of Dnah2 +/Delta (A) and Dnah2 Delta/Delta (B) sperm. Dnah2 Delta/Delta sperm display absence of ( left ) or an irregular ( right ) mitochondrial helical sheath in the midpiece of the sperm tail, along with abnormal head shapes. Arrowheads indicate annulus. H, head; MP, midpiece; and PP, principal piece. (C,D) Transmission electron microscopy images of Dnah2 +/Delta (C) and Dnah2 Delta/Delta (D) sperm. Representative cross-section images of TEM reveal conformational defects in the midpiece of Dnah2 Delta/Delta sperm. Incomplete 9+2 axonemal structure ( left ), delocalized outer dense fibers (ODF, arrow) and microtubule doublet ( middle , arrowhead), and lack of the axonemal components ( right ) were observed in the Dnah2 Delta/Delta sperm. Inner (yellow asterisk) and outer (white asterisk) dynein arm structure were observed from microtubule doublet occasionally ( left , inset; scale bar = 50 nm). Microtubule doublets are numbered with the absence of the corresponding microtubule doublet in red. M, mitochondria. (E) Confocal fluorescence images of axonemal proteins in WT and Dnah2 Delta/Delta sperm. Dnah2 Delta/Delta sperm do not express another IDA component (DNAH1, magenta) and a radial spoke protein (RSPH3, green) but an ODA protein (DNAH9, yellow). Flagella microtubules (AcTub, white) and sperm heads (Hoechst, blue) ser