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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- F54341 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Haptoglobin Antibody / HP
- Antibody type
- Polyclonal
- Description
- This highly specific Haptoglobin antibody is suitable for use in Immunohistochemistry/Western blot/Immunofluorescence/Flow cytometry applications with human and mouse samples.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 0.08 ml, 0.4 ml
- Concentration
- In 1X PBS, pH 7.4, with 0.09% sodium azide
- Storage
- Aliquot the Haptoglobin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human liver, 2) human plasma and 3) mouse plasma lysate with Haptoglobin antibody. Predicted molecular weight ~45 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of fixed and permeabilized human MDA-MB-435 cells with Haptoglobin antibody (green) and DAPI nuclear stain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human hepatocarcinoma tissue with Haptoglobin antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human colon tissue with Haptoglobin antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human MDA-MB-435 cells with Haptoglobin antibody; Blue=isotype control, Green= Haptoglobin antibody.
