PA5-24174

antibody from Invitrogen Antibodies

Targeting: HP

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-24174

Provider

Invitrogen Antibodies

Product name

Haptoglobin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Hypertransaminasemia and liver fibrosis associated with haptoglobin retention and anhaptoglobinemia in a paediatric patient.

Gunzer S, Kraus A, Buchroth I, Grüneberg M, Westermann C, Biskup S, Reunert J, Grünewald I, Marquardt T
Liver international : official journal of the International Association for the Study of the Liver 2021 Oct;41(10):2427-2432

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MDA-MB435 cells using a HP polyclonal antibody (Product # PA5-24174) at a dilution of 1:10-50, followed by a fluor-conjugated goat anti-rabbit secondary antibody (green). Nuclei were stained with DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of Haptoglobin in MDA-MB435 cells. Samples were incubated in Haptoglobin polyclonal antibody (Product # PA5-24174) followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Haptoglobin in paraffin-embedded human hepatocarcinoma tissue. Samples were incubated with Haptoglobin polyclonal antibody (Product # PA5-24174) using a dilution of 1:500 for 1 hour at room temperature followed by an undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH 9.0).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Haptoglobin in paraffin-embedded human colon tissue. Samples were incubated with Haptoglobin polyclonal antibody (Product # PA5-24174) using a dilution of 1:500 for 1 hour at room temperature followed by an undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH 9.0).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of MDA-MB435 cells using a HP polyclonal antibody (Product # PA5-24174) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of Haptoglobin in MDA-MB435 cells (right histogram). Samples were incubated with Haptoglobin polyclonal antibody (Product # PA5-24174) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control cell (left histogram).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

2 FIGURE Immunohistochemistry for immunodetection of Hp in liver biopsy tissue and Western Blot. 2.1 Immunohistochemistry for immunodetection of Hp in liver biopsy tissue. A, Control tissue stained with the monoclonal anti-haptoglobin antibody. Positive staining reaction shows (brown) presence of haptoglobin in the cytoplasm of hepatocytes. Original magnification 400x. B, The monoclonal antibody is not able to detect haptoglobin in the patient-derived tissue. Original magnification 400x. C, Control tissue stained with the polyclonal anti-haptoglobin antibody. Positive staining reaction shows presence of haptoglobin in the hepatocytes. Original magnification 400x. D, Patient`s tissue stained with the polyclonal anti-haptoglobin antibody. Positive staining reaction shows presence of haptoglobin in the hepatocytes. Original magnification 400x. 2.2 Western Blot detection of Haptoglobin in blood serum. C1, C2 = control serum of healthy individuals (m/f) show normal level of haptoglobin. F = patient`s father, shows low level of haptoglobin. P = patient shows no serum haptoglobin. alpha-chain (alpha-2) = 18 kDa. beta-chain = 40 kDa