Ab179921

antibody from Aladdin Scientific

Targeting: TUBB M40, MGC16435, OK/SW-cl.56, Tubb5

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

Ab179921

Provider

Aladdin Scientific

Product name

beta Tubulin Mouse mAb

Antibody type

Monoclonal

Description

ApplicationWB: 1/2,000-1/10,0000; Predicted molecular weight: 50 kDaFlow: 0.002 _g/mL - 0.25 _g/mLProtein Function:Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

AB01/1E7

Vial size

100_l,10_l,1ml,20_l,50_l

Concentration

1 mg/ml

Storage

Store at 4¡C short term (1-2 weeks). Store at -20¡C or -80¡C. Avoid freeze / thaw cycle.

Western blot

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

beta Tubulin Mouse mAb (Ab179921) - Western Blot All lanes: beta Tubulin Antibody (Ab179921) at 0.05 μg/mL Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution Predicted band size: 50.0 kDa Observed band size: 50.0 kDa Exposure time: 90.0 s 

Flow cytometry

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

beta Tubulin Mouse mAb (Ab179921) - Flow Cytometry Overlay histogram showing HeLa cells stained with beta Tubulin Mouse mAb (Ab179921) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (FITC) (Ab156254) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).