TA324820

antibody from OriGene

Targeting: CUX1 CASP, CDP, CDP/Cut, CDP/Cux, CDP1, Clox, CUT, CUTL1, CUX, Cux/CDP, GOLIM6

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA324820

Provider

OriGene

Product name

Rabbit polyclonal CUX1 Antibody (C-term)

Antibody type

Polyclonal

Description

Rabbit polyclonal CUX1 Antibody (C-term)

Host

Rabbit

Conjugate

Unconjugated

Epitope

CUX1

Isotype

IgG

Antibody clone number

NULL

Vial size

400 µl

Concentration

0.5 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

CUX1 Antibody (C-term) (Cat. #TA324820) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the CUX1 antibody detected the CUX1 protein (arrow).

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

IF image of Hela cell stained with CUX1 Antibody (C-term)(Cat#TA324820).Hela cells were incubated with CUX1 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . CUX1 immunoreactivity is localized to Nucleus significantly.

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

CUX1 Antibody (C-term) (Cat. #TA324820) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CUX1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Validation comment

IHC