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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
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- Product number
- PA5-37866 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HOXA10 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with mouse, pig and cow based on sequence homology.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of HOXA10 in human skeletal muscle lysate was performed using 35 µg protein in RIPA buffer. Lysates were probed with a HOXA10 polyclonal antibody (Product # PA5-37866) at a dilution of 0.2 µg/mL and incubated for 1 hour, followed by chemiluminescent detection.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HOXA10 by a HOXA10 monoclonal antibody (Product # PA5-37866) at a concentration of 0.3 µg/mL. Mouse Skeletal Muscle lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of HOXA10 in paraffin embedded human testis using a HOXA10 polyclonal antibody (Product #PA5-37866) at a concentration of 7 µg/mL. Heat induced antigen retrieval was performed with pH 6 buffered citrate. Samples were then stained in horseradish peroxidase.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of HOXA10 in paraffin embedded human testis using a HOXA10 polyclonal antibody (Product #PA5-37866) at a concentration of 7 µg/mL. Heat induced antigen retrieval was performed with pH 6 buffered citrate. Samples were then stained in horseradish peroxidase.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of HOXA10 in paraffin-embedded human testis following steamed antigen retrieval with a citrate buffer, pH 6, using a HOXA10 polyclonal antibody (Product # PA5-37866) at a dilution of 5 µg/mL. Detection was performed using an AP-conjugated secondary antibody.
