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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [3]
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- Product number
- MA5-28627 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- WBP1L Monoclonal Antibody (OPAL1-01)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Description
- This antibody recognizes an intracellular epitope of OPAL1 (outcome predictor in acute leukemia 1), a transmembrane adaptor protein expressed mainly by megakaryocytes and platelets. Western Blot: non-reducing conditions recommended.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OPAL1-01
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4°C, do not freeze
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blotting analysis of human OPAL1 using mouse Monoclonal antibody OPAL1-01 (Product # MA5-28627) on lysates of HEK-293T/OPAL1 transfectants, and of murine splenocytes and HEK-293T cells (negative controls) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2µg/mL of mouse anti-OPAL1 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. OPAL1 was detected at around 45-60kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of WBP1L in OPAL-transfected HEK293 cells using mouse monoclonal antibody OPAL1-01.. Sample was incubated with WBP1L monoclonal antibody (Product # MA5-28627).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of OPAL-transfected HEK293 cells using mouse monoclonal antibody OPAL1-01 Monoclonal antibody (Product # MA5-28627).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of OPAL-transfected HEK293 cells using mouse monoclonal antibody OPAL1-01 Monoclonal antibody (Product # MA5-28627).
