Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [2]
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Validation data
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- Product number
- PA5-48211 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SMTNL1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SMTNL1 in RD whole cell lysate. Samples were incubated with SMTNL1 polyclonal antibody (Product # PA5-48211) using a dilution of 1:2,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Predicted band size :43 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of SMTNL1 in K562 cells (right histogram) compared to a negative control cell (left histogram). Cells were probed with a SMTNL1 Antibody (Center) (Product # PA5-48211). Following incubation with the primary antibody, cells were incubated with FITC-conjugated goat-anti-rabbit secondary antibodies.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of SMTNL1 in K562 cells (right histogram). Samples were incubated with SMTNL1 polyclonal antibody (Product # PA5-48211) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control cell (left histogram).