MA5-18160

antibody from Invitrogen Antibodies

Targeting: FOXP3 AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID

Western blot (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-18160

Provider

Invitrogen Antibodies

Product name

FOXP3 Monoclonal Antibody (3G3), Alexa Fluor™ 647

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Red dye

Isotype

IgG

Antibody clone number

3G3

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

4° C, store in dark, DO NOT FREEZE!

References

Submitted references

A molybdenum oxide-based degradable nanosheet for combined chemo-photothermal therapy to improve tumor immunosuppression and suppress distant tumors and lung metastases.

Qiu N, Yang X, Zhang Y, Zhang J, Ji J, Zhang Y, Kong X, Xi Y, Liu D, Ye L, Zhai G
Journal of nanobiotechnology 2021 Dec 19;19(1):428

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Type I Interferons Suppress Anti-parasitic Immunity and Can Be Targeted to Improve Treatment of Visceral Leishmaniasis.

Kumar R, Bunn PT, Singh SS, Ng SS, Montes de Oca M, De Labastida Rivera F, Chauhan SB, Singh N, Faleiro RJ, Edwards CL, Frame TCM, Sheel M, Austin RJ, Lane SW, Bald T, Smyth MJ, Hill GR, Best SE, Haque A, Corvino D, Waddell N, Koufariotis L, Mukhopadhay P, Rai M, Chakravarty J, Singh OP, Sacks D, Nylen S, Uzonna J, Sundar S, Engwerda CR
Cell reports 2020 Feb 25;30(8):2512-2525.e9

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of FOXP3 in human peripheral blood cells (gated on CD4+ cells) using FoxP3 monoclonal antibody (Product # MA5-18160).

Conjugate

Red dye

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Separation of FoxP3 positive CD25 positive CD4 positive lymphocytes (red-filled) from FoxP3 negative CD25 negative CD4 positive lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human FoxP3 (3G3) Alexa Fluor® 647 Monoclonal antibody (Product # MA5-18160), concentration in sample 3 µg/mL.

Conjugate

Red dye

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human FoxP3 (3G3) Alexa Fluor® 647Monoclonal antibody (Product # MA5-18160), concentration in sample 3 µg/mL.

Conjugate

Red dye

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry multicolor surface staining of human CD4 positive lymphocytes using anti-human CD25 (MEM-181) PE antibody (20 µL reagent / 100 µL of peripheral whole blood) and intracellular staining of human CD4 positive lymphocytes using anti-human FoxP3 (3G3) Alexa Fluor® 647 Monoclonal antibody (Product # MA5-18160), concentration in sample 3 µg/mL.

Conjugate

Red dye

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 11 The mechanism of distant antitumor effect analysis. The flow cytometry images of DCs (CD80 + CD86 + ) mature ( A ) and quantitative analysis ( B ); flow cytometry ( C ) and quantitative analysis ( D , E ) of activated CD3 + CD4 + CD8 + T cells after each treatment; the population of CD4 + Foxp3 + Tregs in the spleen according to flow cytometry ( F ) and the ratio of CD3 + CD8 + cytotoxic T cells versus CD3 + CD4 + Foxp3 + Tregs ( G ); flow cytometry analysis of CD45 + , F4/80 + ( H ) and quantitative analysis ( I , J ) in the distant tumor

Conjugate

Red dye