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LearnFPK8214P-025
antibody from R&D Systems
Targeting: FOXP3
AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Flow cytometry [2]
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- Product number
- FPK8214P-025 - Provider product page
- Provider
- R&D Systems
- Product name
- FlowX Human/Mouse FoxP3 PE Staining Kit
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from cell culture supernatant. Detects human and mouse FoxP3 in flow cytometry.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Antigen sequence
Q9BZS1- Isotype
- IgG
- Antibody clone number
- 1054C
- Vial size
- 25 Tests
- Storage
- Store the unopened product at 2 to 8 °C. Do not use past expiration date. Protect from light.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of FoxP3 in Human PBMC Natural Tregs by Flow Cytometry. Human peripheral blood mononuclear cell (PBMC) natural regulatory T cells (Tregs) were surface stained with (A) Mouse Anti-Human CD4 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3791F) and (B) Mouse Anti-Human IL-2 R alpha (CD25) APC-conjugated Monoclonal Antibody (Catalog # FAB1020A), followed by intracellular staining using Rabbit Anti-Human/Mouse FoxP3 PE-conjugated Monoclonal Antibody (Catalog # IC8214P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). Cells were gated on lymphocytes.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of FoxP3 in C57BL/6 Mouse Splenocyte Natural Tregs by Flow Cytometry. C57BL/6 mouse splenocyte natural regulatory T cells (Tregs) were surface stained with (A) Rat Anti-Mouse CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB554A) and (B) Rat Anti-Mouse IL-2 R alpha (CD25) APC-conjugated Monoclonal Antibody (Catalog # FAB2438A) followed by intracellular staining using Rabbit Anti-Human/Mouse FoxP3 PE-conjugated Monoclonal Antibody (Catalog # IC8214P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). Cells were gated on lymphocytes.
