Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [2]
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Validation data
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- Product number
- IC8214N-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse FoxP3 Alexa Fluor® 700-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from cell culture supernatant. Detects human and mouse FoxP3 in flow cytometry.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Near infrared dye
- Antigen sequence
Q9BZS1
- Isotype
- IgG
- Antibody clone number
- 1054C
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Multiplex immunoassay characterization and species comparison of inflammation in acute and non-acute ischemic infarcts in human and mouse brain tissue.
Nguyen TV, Frye JB, Zbesko JC, Stepanovic K, Hayes M, Urzua A, Serrano G, Beach TG, Doyle KP
Acta neuropathologica communications 2016 Sep 6;4(1):100
Acta neuropathologica communications 2016 Sep 6;4(1):100
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of FoxP3 in Human PBMC Natural Tregs by Flow Cytometry. Human peripheral blood mononuclear cell (PBMC) natural regulatory T cells (Tregs) were surface stained with (A) Mouse Anti-Human CD4 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3791F) and (B) Mouse Anti-Human IL-2 R alpha (CD25) PE-conjugated Monoclonal Antibody (Catalog # FAB1020P), followed by intracellular staining using Rabbit Anti-Human/Mouse FoxP3 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # IC8214N). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). Cells were gated on lymphocytes.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of FoxP3 in C57BL/6 Mouse Splenocyte Natural Tregs by Flow Cytometry. C57BL/6 mouse splenocyte natural regulatory T cells (Tregs) were surface stained with (A) Rat Anti-Mouse CD4 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB554F) and (B) Rat Anti-Mouse IL-2 R alpha (CD25) PE-conjugated Monoclonal Antibody (Catalog # FAB2438P) followed by intracellular staining using Rabbit Anti-Human/Mouse FoxP3 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # IC8214N). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). Cells were gated on lymphocytes.