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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- R34634 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Scurfin Antibody
- Antibody type
- Polyclonal
- Antigen
- Amino acids KRSQRPNKCSNP were used as the immunogen for this Scurfin antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store the Scurfin antibody at -20°C.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of fixed and permeabilized mouse NIH 3T3 cells with Scurfin antibody (green) at 10ug/ml, phalloidin (red) and DAPI nuclear stain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE mouse thymus with (left) and without (right) Scurfin antibody at 2ug/ml. HIER: steamed with pH9 Tris/EDTA buffer, HRP-staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE mouse spleen tissue with Scurfin antibody at 10ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Negative control: IHC staining of FFPE mouse spleen tissue without Scurfin antibody. Required HIER: steamed antigen retrieval with pH6 citrate buffer.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- FACS testing of fixed and permeabilized mouse NIH 3T3 cells with Scurfin antibody (blue) at 10ug/million cells in 0.2ml and naive goat Ig (black).
