694802

antibody from BioLegend

Targeting: FOXP3 AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

694802

Provider

BioLegend

Proper citation

BioLegend Cat#694802, RRID:AB_2650734

Product name

Purified anti-human FOXP3 Delta 2 (exon 2 deleted)

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

A15080A

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Total cell lysates (15 µg protein) from 293E untransfected (lane 1), 293E transfected with human FOXP3 (lane 2), and 293E transfected with human FOXP3 Delta2 (lane 3) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 0.1 µg/mL purified anti-FOXP3 Delta 2 (exon 2 deleted) antibody (clone A15080A). Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody (clone Poly4053) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.

Conjugate

Unconjugated