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antibody from Invitrogen Antibodies
Targeting: FOXP3
AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID
Immunohistochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [105]
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- Flow cytometry [1]
- Other assay [32]
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- Product number
- 11-4776-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FOXP3 Monoclonal Antibody (PCH101), FITC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: eBioscience offers a panel of monoclonal antibodies to different epitopes of human Foxp3, providing useful tools for investigating the complete expression pattern of Foxp3 at the protein level, and discerning the precise subsets of Foxp3^+ cells. Please contact tech@ebioscience.com or 888.810.6168 for any additional assistance. The PCH101 antibody reacts with the amino terminus of human foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells. Intracellular staining of human peripheral blood mononuclear cells (PBMCs) with PCH101 antibody using the anti-human Foxp3 Staining Set and protocol reveals approximately 0.5-4% of lymphocytes staining, with the majority of staining occurring in the CD25^bright population. This is subject to donor variability. PCH101 crossreacts with rhesus, chimpanzee and cynomolgus. We recommend the use of CD4 (OKT4, Product # 11-0048-42 , or RPA-T4, Product # 11-0049-42 , depending on the species) and CD25 (BC96, Product # 17-0259-42). Applications Reported: This PCH101 antibody has been reported for use in intracellular staining followed by flow cytometric analysis and immunohistology (both paraffin and frozen sections). Applications Tested: This PCH101 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Anti-Human Foxp3 FITC Staining Set (Product # 71-5776). This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- PCH101
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references P140 Peptide Leads to Clearance of Autoreactive Lymphocytes and Normalizes Immune Response in Lupus-Prone Mice.
EC-18 prevents autoimmune arthritis by suppressing inflammatory cytokines and osteoclastogenesis.
Mesenchymal stem cells transfer mitochondria to allogeneic Tregs in an HLA-dependent manner improving their immunosuppressive activity.
SARS-CoV-2 infection paralyzes cytotoxic and metabolic functions of the immune cells.
Standardized 11-color flow cytometry panel for the functional phenotyping of human T regulatory cells.
Effects of Desflurane and Sevoflurane anesthesia on regulatory T cells in patients undergoing living donor kidney transplantation: a randomized intervention trial.
Decreased effector regulatory T cells and increased activated CD4(+) T cells in premature ovarian insufficiency.
Secretory High-Mobility Group Box 1 Protein Affects Regulatory T Cell Differentiation in Neuroblastoma Microenvironment In Vitro.
Functionalization-dependent effects of cellulose nanofibrils on tolerogenic mechanisms of human dendritic cells.
An immunoregulatory and tissue-residency program modulated by c-MAF in human T(H)17 cells.
Clinical significance of CCR7(+)CD8(+) T cells in kidney transplant recipients with allograft rejection.
CD8+ T cells with high TGF‑β1 expression cause lymph node fibrosis following HIV infection.
IL-6 receptor blockade corrects defects of XIAP-deficient regulatory T cells.
Circulating exosomes suppress the induction of regulatory T cells via let-7i in multiple sclerosis.
Effect of TGF-β1 on blood CD4(+)CD25(high) regulatory T cell proliferation and Foxp3 expression during non-small cell lung cancer blood metastasis.
Fc Effector Function Contributes to the Activity of Human Anti-CTLA-4 Antibodies.
Protective effect of 1α,25-dihydroxyvitamin D3 on effector CD4+ T cell induced injury in human renal proximal tubular epithelial cells.
The Influence of Cytomegalovirus on Expression of HLA-G and its Ligand KIR2DL4 by Human Peripheral Blood Leucocyte Subsets.
HDAC inhibition potentiates immunotherapy in triple negative breast cancer.
Blocking the recruitment of naive CD4(+) T cells reverses immunosuppression in breast cancer.
T cell subsets: an immunological biomarker to predict progression to clinical arthritis in ACPA-positive individuals.
Safety and immunologic benefits of conversion to sirolimus in kidney transplant recipients with long-term exposure to calcineurin inhibitors.
Equilibrium of Treg/Th17 cells of peripheral blood in syphilitic patients with sero-resistance.
Differentiation of ICOS+ and ICOS- recent thymic emigrant regulatory T cells (RTE T regs) during normal pregnancy, pre-eclampsia and HELLP syndrome.
Pulmonary sarcoidosis is associated with high-level inducible co-stimulator (ICOS) expression on lung regulatory T cells--possible implications for the ICOS/ICOS-ligand axis in disease course and resolution.
Human Head and Neck Squamous Cell Carcinoma-Associated Semaphorin 4D Induces Expansion of Myeloid-Derived Suppressor Cells.
Oxygen Sensing by T Cells Establishes an Immunologically Tolerant Metastatic Niche.
Development of Type 2, But Not Type 1, Leprosy Reactions is Associated with a Severe Reduction of Circulating and In situ Regulatory T-Cells.
Increase of Th17 Cell Phenotype in Kidney Transplant Recipients with Chronic Allograft Dysfunction.
Optimization of adipose tissue-derived mesenchymal stem cells by rapamycin in a murine model of acute graft-versus-host disease.
Regulatory and Helper Follicular T Cells and Antibody Avidity to Simian Immunodeficiency Virus Glycoprotein 120.
Glycolysis controls the induction of human regulatory T cells by modulating the expression of FOXP3 exon 2 splicing variants.
The role of interleukin-1 beta in the pathophysiology of Schnitzler's syndrome.
Induction of Multiple Immune Regulatory Pathways with Differential Impact in HCV/HIV Coinfection.
Tumor-infiltrating immune cell profiles and their change after neoadjuvant chemotherapy predict response and prognosis of breast cancer.
Biallelic loss-of-function mutation in NIK causes a primary immunodeficiency with multifaceted aberrant lymphoid immunity.
Incomplete normalization of regulatory t-cell frequency in the gut mucosa of Colombian HIV-infected patients receiving long-term antiretroviral treatment.
Human T cells upregulate CD69 after coculture with xenogeneic genetically-modified pig mesenchymal stromal cells.
Human secondary lymphoid organs typically contain polyclonally-activated proliferating regulatory T cells.
IL-7 modulates in vitro and in vivo human memory T regulatory cell functions through the CD39/ATP axis.
The immune cell infiltrate populating meningiomas is composed of mature, antigen-experienced T and B cells.
Intratumoral regulatory T cells upregulate immunosuppressive molecules in head and neck cancer patients.
CD4⁺CD73⁺ T cells are associated with lower T-cell activation and C reactive protein levels and are depleted in HIV-1 infection regardless of viral suppression.
Subinfectious hepatitis C virus exposures suppress T cell responses against subsequent acute infection.
T regulatory cells and related immunoregulatory factors in polymorphic light eruption following ultraviolet A1 challenge.
Human Treg responses allow sustained recombinant adeno-associated virus-mediated transgene expression.
Effects of adjuvant chemoradiotherapy on the frequency and function of regulatory T cells in patients with head and neck cancer.
DR(high+)CD45RA(-)-Tregs potentially affect the suppressive activity of the total Treg pool in renal transplant patients.
The activation pattern of blood leukocytes in head and neck squamous cell carcinoma is correlated to survival.
Seasonal variation in vitamin D₃ levels is paralleled by changes in the peripheral blood human T cell compartment.
Impaired function of regulatory T cells in cord blood of children of allergic mothers.
Term and preterm labor: decreased suppressive activity and changes in composition of the regulatory T-cell pool.
Increased expression of regulatory T cells and down-regulatory molecules in lepromatous leprosy.
Graft invariant natural killer T-cell dose predicts risk of acute graft-versus-host disease in allogeneic hematopoietic stem cell transplantation.
Immunological parameters associated with the development of allergic rhinitis: a preliminary prospective study.
Induction of endometriosis alters the peripheral and endometrial regulatory T cell population in the non-human primate.
The vaccine-site microenvironment induced by injection of incomplete Freund's adjuvant, with or without melanoma peptides.
IRX-2, a novel biologic, favors the expansion of T effector over T regulatory cells in a human tumor microenvironment model.
Suppression of tumour-specific CD4⁺ T cells by regulatory T cells is associated with progression of human colorectal cancer.
B cell-associated immune profiles in patients with end-stage renal disease (ESRD).
OMIP-006: phenotypic subset analysis of human T regulatory cells via polychromatic flow cytometry.
Choice of resident costimulatory molecule can influence cell fate in human naïve CD4+ T cell differentiation.
Engagement of TLR2 reverses the suppressor function of conjunctiva CD4+CD25+ regulatory T cells and promotes herpes simplex virus epitope-specific CD4+CD25- effector T cell responses.
Separation of human CD4+CD39+ T cells by magnetic beads reveals two phenotypically and functionally different subsets.
Association between regulatory T cell activity and sepsis and outcome of severely burned patients: a prospective, observational study.
Gender specificity of altered human immune cytokine profiles in aging.
Histone/protein deacetylase inhibitors increase suppressive functions of human FOXP3+ Tregs.
CD4+ CD25+ Foxp3+ regulatory T cells, dendritic cells, and circulating cytokines in uncomplicated malaria: do different parasite species elicit similar host responses?
Mechanisms of immune suppression for CD8+ T cells by human corneal endothelial cells via membrane-bound TGFbeta.
Reciprocal granzyme/perforin-mediated death of human regulatory and responder T cells is regulated by interleukin-2 (IL-2).
CD25 appears non essential for human peripheral T(reg) maintenance in vivo.
CD40 signalling induces IL-10-producing, tolerogenic dendritic cells.
Safety and T cell modulating effects of high dose vitamin D3 supplementation in multiple sclerosis.
CD4(+) regulatory T cells in a cynomolgus macaque model of Mycobacterium tuberculosis infection.
FOXP3+ regulatory T cells and T helper 1, T helper 2, and T helper 17 cells in human early pregnancy decidua.
In colorectal cancer mast cells contribute to systemic regulatory T-cell dysfunction.
De novo generation and enhanced suppression of human CD4+CD25+ regulatory T cells by retinoic acid.
Expansion of allospecific regulatory T cells after anergized, mismatched bone marrow transplantation.
The inhibitory Fc gamma IIb receptor dampens TLR4-mediated immune responses and is selectively up-regulated on dendritic cells from rheumatoid arthritis patients with quiescent disease.
Differential responses of human regulatory T cells (Treg) and effector T cells to rapamycin.
Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells.
Vaccine-specific local T cell reactivity in immunotherapy-associated vitiligo in melanoma patients.
The importance of Foxp3 antibody and fixation/permeabilization buffer combinations in identifying CD4+CD25+Foxp3+ regulatory T cells.
CD13+CD4+CD25hi regulatory T cells exhibit higher suppressive function and increase with tumor stage in non-small cell lung cancer patients.
Naturally occurring regulatory T cells show reduced sensitivity toward oxidative stress-induced cell death.
Clinical grade Treg: GMP isolation, improvement of purity by CD127 Depletion, Treg expansion, and Treg cryopreservation.
Resting regulatory CD4 T cells: a site of HIV persistence in patients on long-term effective antiretroviral therapy.
Expression of ICOS on human melanoma-infiltrating CD4+CD25highFoxp3+ T regulatory cells: implications and impact on tumor-mediated immune suppression.
Calcineurin inhibitor withdrawal after renal transplantation with alemtuzumab: clinical outcomes and effect on T-regulatory cells.
Immunological monitoring of renal transplant recipients to predict acute allograft rejection following the discontinuation of tacrolimus.
Human regulatory T cells inhibit polarization of T helper cells toward antigen-presenting cells via a TGF-beta-dependent mechanism.
Enhanced functionality of CD4+CD25(high)FoxP3+ regulatory T cells in the peripheral blood of patients with prostate cancer.
Safety and immunologic response of a viral vaccine to prostate-specific antigen in combination with radiation therapy when metronomic-dose interleukin 2 is used as an adjuvant.
Expansion of CD4+CD25+ suppressive regulatory T cells from rhesus macaque peripheral blood by FN18/antihuman CD28-coated Dynal beads.
IL-15 and dermal fibroblasts induce proliferation of natural regulatory T cells isolated from human skin.
FOXP3 regulates TLR10 expression in human T regulatory cells.
A novel mechanism of action for anti-thymocyte globulin: induction of CD4+CD25+Foxp3+ regulatory T cells.
Mucosal but not peripheral FOXP3+ regulatory T cells are highly increased in untreated HIV infection and normalize after suppressive HAART.
IL-2 administration increases CD4+ CD25(hi) Foxp3+ regulatory T cells in cancer patients.
Foxp3+CD4+CD25+ T cells control virus-specific memory T cells in chimpanzees that recovered from hepatitis C.
Depletion of alloreactive T cells via CD69: implications on antiviral, antileukemic and immunoregulatory T lymphocytes.
Rapamycin, and not cyclosporin A, preserves the highly suppressive CD27+ subset of human CD4+CD25+ regulatory T cells.
Cutting edge: direct suppression of B cells by CD4+ CD25+ regulatory T cells.
Cutting edge: direct suppression of B cells by CD4+ CD25+ regulatory T cells.
Human CD4+ T cells express TLR5 and its ligand flagellin enhances the suppressive capacity and expression of FOXP3 in CD4+CD25+ T regulatory cells.
Schall N, Talamini L, Wilhelm M, Jouvin-Marche E, Muller S
Frontiers in immunology 2022;13:904669
Frontiers in immunology 2022;13:904669
EC-18 prevents autoimmune arthritis by suppressing inflammatory cytokines and osteoclastogenesis.
Park JS, Yang SC, Jeong HY, Lee SY, Ryu JG, Choi JW, Kang HY, Kim SM, Hwang SH, Cho ML, Park SH
Arthritis research & therapy 2022 Nov 17;24(1):254
Arthritis research & therapy 2022 Nov 17;24(1):254
Mesenchymal stem cells transfer mitochondria to allogeneic Tregs in an HLA-dependent manner improving their immunosuppressive activity.
Piekarska K, Urban-Wójciuk Z, Kurkowiak M, Pelikant-Małecka I, Schumacher A, Sakowska J, Spodnik JH, Arcimowicz Ł, Zielińska H, Tymoniuk B, Renkielska A, Siebert J, Słomińska E, Trzonkowski P, Hupp T, Marek-Trzonkowska NM
Nature communications 2022 Feb 14;13(1):856
Nature communications 2022 Feb 14;13(1):856
SARS-CoV-2 infection paralyzes cytotoxic and metabolic functions of the immune cells.
Singh Y, Trautwein C, Fendel R, Krickeberg N, Berezhnoy G, Bissinger R, Ossowski S, Salker MS, Casadei N, Riess O, Deutsche COVID-19 OMICS Initiate (DeCOI)
Heliyon 2021 Jun;7(6):e07147
Heliyon 2021 Jun;7(6):e07147
Standardized 11-color flow cytometry panel for the functional phenotyping of human T regulatory cells.
Manuszak C, Brainard M, Thrash E, Hodi FS, Severgnini M
Journal of biological methods 2020;7(2):e131
Journal of biological methods 2020;7(2):e131
Effects of Desflurane and Sevoflurane anesthesia on regulatory T cells in patients undergoing living donor kidney transplantation: a randomized intervention trial.
Chutipongtanate A, Prukviwat S, Pongsakul N, Srisala S, Kamanee N, Arpornsujaritkun N, Gesprasert G, Apiwattanakul N, Hongeng S, Ittichaikulthol W, Sumethkul V, Chutipongtanate S
BMC anesthesiology 2020 Aug 27;20(1):215
BMC anesthesiology 2020 Aug 27;20(1):215
Decreased effector regulatory T cells and increased activated CD4(+) T cells in premature ovarian insufficiency.
Kobayashi M, Nakashima A, Yoshino O, Yoshie M, Ushijima A, Ito M, Ono Y, Shima T, Kawamura K, Ishizuka B, Saito S
American journal of reproductive immunology (New York, N.Y. : 1989) 2019 Jun;81(6):e13125
American journal of reproductive immunology (New York, N.Y. : 1989) 2019 Jun;81(6):e13125
Secretory High-Mobility Group Box 1 Protein Affects Regulatory T Cell Differentiation in Neuroblastoma Microenvironment In Vitro.
Vanichapol T, Chiangjong W, Panachan J, Anurathapan U, Chutipongtanate S, Hongeng S
Journal of oncology 2018;2018:7946021
Journal of oncology 2018;2018:7946021
Functionalization-dependent effects of cellulose nanofibrils on tolerogenic mechanisms of human dendritic cells.
Tomić S, Ilić N, Kokol V, Gruden-Movsesijan A, Mihajlović D, Bekić M, Sofronić-Milosavljević L, Čolić M, Vučević D
International journal of nanomedicine 2018;13:6941-6960
International journal of nanomedicine 2018;13:6941-6960
An immunoregulatory and tissue-residency program modulated by c-MAF in human T(H)17 cells.
Aschenbrenner D, Foglierini M, Jarrossay D, Hu D, Weiner HL, Kuchroo VK, Lanzavecchia A, Notarbartolo S, Sallusto F
Nature immunology 2018 Oct;19(10):1126-1136
Nature immunology 2018 Oct;19(10):1126-1136
Clinical significance of CCR7(+)CD8(+) T cells in kidney transplant recipients with allograft rejection.
Kim KW, Kim BM, Doh KC, Cho ML, Yang CW, Chung BH
Scientific reports 2018 Jun 11;8(1):8827
Scientific reports 2018 Jun 11;8(1):8827
CD8+ T cells with high TGF‑β1 expression cause lymph node fibrosis following HIV infection.
Huang L, Deng J, Xu W, Wang H, Shi L, Wu F, Wu D, Nei W, Zhao M, Mao P, Zhou X
Molecular medicine reports 2018 Jul;18(1):77-86
Molecular medicine reports 2018 Jul;18(1):77-86
IL-6 receptor blockade corrects defects of XIAP-deficient regulatory T cells.
Hsieh WC, Hsu TS, Chang YJ, Lai MZ
Nature communications 2018 Jan 31;9(1):463
Nature communications 2018 Jan 31;9(1):463
Circulating exosomes suppress the induction of regulatory T cells via let-7i in multiple sclerosis.
Kimura K, Hohjoh H, Fukuoka M, Sato W, Oki S, Tomi C, Yamaguchi H, Kondo T, Takahashi R, Yamamura T
Nature communications 2018 Jan 2;9(1):17
Nature communications 2018 Jan 2;9(1):17
Effect of TGF-β1 on blood CD4(+)CD25(high) regulatory T cell proliferation and Foxp3 expression during non-small cell lung cancer blood metastasis.
Hu Y, Qi W, Sun L, Zhou H, Zhou B, Yang Z
Experimental and therapeutic medicine 2018 Aug;16(2):1403-1410
Experimental and therapeutic medicine 2018 Aug;16(2):1403-1410
Fc Effector Function Contributes to the Activity of Human Anti-CTLA-4 Antibodies.
Arce Vargas F, Furness AJS, Litchfield K, Joshi K, Rosenthal R, Ghorani E, Solomon I, Lesko MH, Ruef N, Roddie C, Henry JY, Spain L, Ben Aissa A, Georgiou A, Wong YNS, Smith M, Strauss D, Hayes A, Nicol D, O'Brien T, Mårtensson L, Ljungars A, Teige I, Frendéus B, TRACERx Melanoma, TRACERx Renal, TRACERx Lung consortia, Pule M, Marafioti T, Gore M, Larkin J, Turajlic S, Swanton C, Peggs KS, Quezada SA
Cancer cell 2018 Apr 9;33(4):649-663.e4
Cancer cell 2018 Apr 9;33(4):649-663.e4
Protective effect of 1α,25-dihydroxyvitamin D3 on effector CD4+ T cell induced injury in human renal proximal tubular epithelial cells.
Chung BH, Kim BM, Doh KC, Cho ML, Kim KW, Yang CW
PloS one 2017;12(2):e0172536
PloS one 2017;12(2):e0172536
The Influence of Cytomegalovirus on Expression of HLA-G and its Ligand KIR2DL4 by Human Peripheral Blood Leucocyte Subsets.
Albayati Z, Alyami A, Alomar S, Middleton D, Bonnett L, Aleem S, Flanagan BF, Christmas SE
Scandinavian journal of immunology 2017 Nov;86(5):396-407
Scandinavian journal of immunology 2017 Nov;86(5):396-407
HDAC inhibition potentiates immunotherapy in triple negative breast cancer.
Terranova-Barberio M, Thomas S, Ali N, Pawlowska N, Park J, Krings G, Rosenblum MD, Budillon A, Munster PN
Oncotarget 2017 Dec 26;8(69):114156-114172
Oncotarget 2017 Dec 26;8(69):114156-114172
Blocking the recruitment of naive CD4(+) T cells reverses immunosuppression in breast cancer.
Su S, Liao J, Liu J, Huang D, He C, Chen F, Yang L, Wu W, Chen J, Lin L, Zeng Y, Ouyang N, Cui X, Yao H, Su F, Huang JD, Lieberman J, Liu Q, Song E
Cell research 2017 Apr;27(4):461-482
Cell research 2017 Apr;27(4):461-482
T cell subsets: an immunological biomarker to predict progression to clinical arthritis in ACPA-positive individuals.
Hunt L, Hensor EM, Nam J, Burska AN, Parmar R, Emery P, Ponchel F
Annals of the rheumatic diseases 2016 Oct;75(10):1884-9
Annals of the rheumatic diseases 2016 Oct;75(10):1884-9
Safety and immunologic benefits of conversion to sirolimus in kidney transplant recipients with long-term exposure to calcineurin inhibitors.
Yu JH, Kim KW, Kim BM, Chung BH, Cho ML, Choi BS, Park CW, Kim YS, Yang CW
The Korean journal of internal medicine 2016 May;31(3):552-9
The Korean journal of internal medicine 2016 May;31(3):552-9
Equilibrium of Treg/Th17 cells of peripheral blood in syphilitic patients with sero-resistance.
Zhao J, Ma J, Zhang X, Li Q, Yang X
Experimental and therapeutic medicine 2016 Jun;11(6):2300-2304
Experimental and therapeutic medicine 2016 Jun;11(6):2300-2304
Differentiation of ICOS+ and ICOS- recent thymic emigrant regulatory T cells (RTE T regs) during normal pregnancy, pre-eclampsia and HELLP syndrome.
Wagner MI, Jöst M, Spratte J, Schaier M, Mahnke K, Meuer S, Zeier M, Steinborn A
Clinical and experimental immunology 2016 Jan;183(1):129-42
Clinical and experimental immunology 2016 Jan;183(1):129-42
Pulmonary sarcoidosis is associated with high-level inducible co-stimulator (ICOS) expression on lung regulatory T cells--possible implications for the ICOS/ICOS-ligand axis in disease course and resolution.
Sakthivel P, Grunewald J, Eklund A, Bruder D, Wahlström J
Clinical and experimental immunology 2016 Feb;183(2):294-306
Clinical and experimental immunology 2016 Feb;183(2):294-306
Human Head and Neck Squamous Cell Carcinoma-Associated Semaphorin 4D Induces Expansion of Myeloid-Derived Suppressor Cells.
Younis RH, Han KL, Webb TJ
Journal of immunology (Baltimore, Md. : 1950) 2016 Feb 1;196(3):1419-29
Journal of immunology (Baltimore, Md. : 1950) 2016 Feb 1;196(3):1419-29
Oxygen Sensing by T Cells Establishes an Immunologically Tolerant Metastatic Niche.
Clever D, Roychoudhuri R, Constantinides MG, Askenase MH, Sukumar M, Klebanoff CA, Eil RL, Hickman HD, Yu Z, Pan JH, Palmer DC, Phan AT, Goulding J, Gattinoni L, Goldrath AW, Belkaid Y, Restifo NP
Cell 2016 Aug 25;166(5):1117-1131.e14
Cell 2016 Aug 25;166(5):1117-1131.e14
Development of Type 2, But Not Type 1, Leprosy Reactions is Associated with a Severe Reduction of Circulating and In situ Regulatory T-Cells.
Vieira AP, Trindade MÂ, Pagliari C, Avancini J, Sakai-Valente NY, Duarte AJ, Benard G
The American journal of tropical medicine and hygiene 2016 Apr;94(4):721-7
The American journal of tropical medicine and hygiene 2016 Apr;94(4):721-7
Increase of Th17 Cell Phenotype in Kidney Transplant Recipients with Chronic Allograft Dysfunction.
Chung BH, Kim KW, Kim BM, Doh KC, Cho ML, Yang CW
PloS one 2015;10(12):e0145258
PloS one 2015;10(12):e0145258
Optimization of adipose tissue-derived mesenchymal stem cells by rapamycin in a murine model of acute graft-versus-host disease.
Kim KW, Moon SJ, Park MJ, Kim BM, Kim EK, Lee SH, Lee EJ, Chung BH, Yang CW, Cho ML
Stem cell research & therapy 2015 Oct 23;6:202
Stem cell research & therapy 2015 Oct 23;6:202
Regulatory and Helper Follicular T Cells and Antibody Avidity to Simian Immunodeficiency Virus Glycoprotein 120.
Blackburn MJ, Zhong-Min M, Caccuri F, McKinnon K, Schifanella L, Guan Y, Gorini G, Venzon D, Fenizia C, Binello N, Gordon SN, Miller CJ, Franchini G, Vaccari M
Journal of immunology (Baltimore, Md. : 1950) 2015 Oct 1;195(7):3227-36
Journal of immunology (Baltimore, Md. : 1950) 2015 Oct 1;195(7):3227-36
Glycolysis controls the induction of human regulatory T cells by modulating the expression of FOXP3 exon 2 splicing variants.
De Rosa V, Galgani M, Porcellini A, Colamatteo A, Santopaolo M, Zuchegna C, Romano A, De Simone S, Procaccini C, La Rocca C, Carrieri PB, Maniscalco GT, Salvetti M, Buscarinu MC, Franzese A, Mozzillo E, La Cava A, Matarese G
Nature immunology 2015 Nov;16(11):1174-84
Nature immunology 2015 Nov;16(11):1174-84
The role of interleukin-1 beta in the pathophysiology of Schnitzler's syndrome.
de Koning HD, Schalkwijk J, Stoffels M, Jongekrijg J, Jacobs JF, Verwiel E, Koenen HJ, Preijers F, Holzinger D, Joosten I, van der Meer JW, Simon A
Arthritis research & therapy 2015 Jul 22;17(1):187
Arthritis research & therapy 2015 Jul 22;17(1):187
Induction of Multiple Immune Regulatory Pathways with Differential Impact in HCV/HIV Coinfection.
Cho H, Kikuchi M, Li Y, Nakamoto N, Amorosa VK, Valiga ME, Chang KM
Frontiers in immunology 2014;5:265
Frontiers in immunology 2014;5:265
Tumor-infiltrating immune cell profiles and their change after neoadjuvant chemotherapy predict response and prognosis of breast cancer.
García-Martínez E, Gil GL, Benito AC, González-Billalabeitia E, Conesa MA, García García T, García-Garre E, Vicente V, Ayala de la Peña F
Breast cancer research : BCR 2014 Nov 29;16(6):488
Breast cancer research : BCR 2014 Nov 29;16(6):488
Biallelic loss-of-function mutation in NIK causes a primary immunodeficiency with multifaceted aberrant lymphoid immunity.
Willmann KL, Klaver S, Doğu F, Santos-Valente E, Garncarz W, Bilic I, Mace E, Salzer E, Conde CD, Sic H, Májek P, Banerjee PP, Vladimer GI, Haskoloğlu S, Bolkent MG, Küpesiz A, Condino-Neto A, Colinge J, Superti-Furga G, Pickl WF, van Zelm MC, Eibel H, Orange JS, Ikincioğulları A, Boztuğ K
Nature communications 2014 Nov 19;5:5360
Nature communications 2014 Nov 19;5:5360
Incomplete normalization of regulatory t-cell frequency in the gut mucosa of Colombian HIV-infected patients receiving long-term antiretroviral treatment.
Rueda CM, Velilla PA, Chougnet CA, Rugeles MT
PloS one 2013;8(8):e71062
PloS one 2013;8(8):e71062
Human T cells upregulate CD69 after coculture with xenogeneic genetically-modified pig mesenchymal stromal cells.
Li J, Andreyev O, Chen M, Marco M, Iwase H, Long C, Ayares D, Shen Z, Cooper DK, Ezzelarab MB
Cellular immunology 2013 Sep-Oct;285(1-2):23-30
Cellular immunology 2013 Sep-Oct;285(1-2):23-30
Human secondary lymphoid organs typically contain polyclonally-activated proliferating regulatory T cells.
Peters JH, Koenen HJ, Fasse E, Tijssen HJ, Ijzermans JN, Groenen PJ, Schaap NP, Kwekkeboom J, Joosten I
Blood 2013 Sep 26;122(13):2213-23
Blood 2013 Sep 26;122(13):2213-23
IL-7 modulates in vitro and in vivo human memory T regulatory cell functions through the CD39/ATP axis.
Younas M, Hue S, Lacabaratz C, Guguin A, Wiedemann A, Surenaud M, Beq S, Croughs T, Lelièvre JD, Lévy Y
Journal of immunology (Baltimore, Md. : 1950) 2013 Sep 15;191(6):3161-8
Journal of immunology (Baltimore, Md. : 1950) 2013 Sep 15;191(6):3161-8
The immune cell infiltrate populating meningiomas is composed of mature, antigen-experienced T and B cells.
Fang L, Lowther DE, Meizlish ML, Anderson RC, Bruce JN, Devine L, Huttner AJ, Kleinstein SH, Lee JY, Stern JN, Yaari G, Lovato L, Cronk KM, O'Connor KC
Neuro-oncology 2013 Nov;15(11):1479-90
Neuro-oncology 2013 Nov;15(11):1479-90
Intratumoral regulatory T cells upregulate immunosuppressive molecules in head and neck cancer patients.
Jie HB, Gildener-Leapman N, Li J, Srivastava RM, Gibson SP, Whiteside TL, Ferris RL
British journal of cancer 2013 Nov 12;109(10):2629-35
British journal of cancer 2013 Nov 12;109(10):2629-35
CD4⁺CD73⁺ T cells are associated with lower T-cell activation and C reactive protein levels and are depleted in HIV-1 infection regardless of viral suppression.
Schuler PJ, Macatangay BJ, Saze Z, Jackson EK, Riddler SA, Buchanan WG, Hilldorfer BB, Mellors JW, Whiteside TL, Rinaldo CR
AIDS (London, England) 2013 Jun 19;27(10):1545-55
AIDS (London, England) 2013 Jun 19;27(10):1545-55
Subinfectious hepatitis C virus exposures suppress T cell responses against subsequent acute infection.
Park SH, Veerapu NS, Shin EC, Biancotto A, McCoy JP, Capone S, Folgori A, Rehermann B
Nature medicine 2013 Dec;19(12):1638-42
Nature medicine 2013 Dec;19(12):1638-42
T regulatory cells and related immunoregulatory factors in polymorphic light eruption following ultraviolet A1 challenge.
Gambichler T, Terras S, Kampilafkos P, Kreuter A, Skrygan M
The British journal of dermatology 2013 Dec;169(6):1288-94
The British journal of dermatology 2013 Dec;169(6):1288-94
Human Treg responses allow sustained recombinant adeno-associated virus-mediated transgene expression.
Mueller C, Chulay JD, Trapnell BC, Humphries M, Carey B, Sandhaus RA, McElvaney NG, Messina L, Tang Q, Rouhani FN, Campbell-Thompson M, Fu AD, Yachnis A, Knop DR, Ye GJ, Brantly M, Calcedo R, Somanathan S, Richman LP, Vonderheide RH, Hulme MA, Brusko TM, Wilson JM, Flotte TR
The Journal of clinical investigation 2013 Dec;123(12):5310-8
The Journal of clinical investigation 2013 Dec;123(12):5310-8
Effects of adjuvant chemoradiotherapy on the frequency and function of regulatory T cells in patients with head and neck cancer.
Schuler PJ, Harasymczuk M, Schilling B, Saze Z, Strauss L, Lang S, Johnson JT, Whiteside TL
Clinical cancer research : an official journal of the American Association for Cancer Research 2013 Dec 1;19(23):6585-96
Clinical cancer research : an official journal of the American Association for Cancer Research 2013 Dec 1;19(23):6585-96
DR(high+)CD45RA(-)-Tregs potentially affect the suppressive activity of the total Treg pool in renal transplant patients.
Schaier M, Seissler N, Schmitt E, Meuer S, Hug F, Zeier M, Steinborn A
PloS one 2012;7(3):e34208
PloS one 2012;7(3):e34208
The activation pattern of blood leukocytes in head and neck squamous cell carcinoma is correlated to survival.
Millrud CR, Månsson Kvarnhammar A, Uddman R, Björnsson S, Riesbeck K, Cardell LO
PloS one 2012;7(12):e51120
PloS one 2012;7(12):e51120
Seasonal variation in vitamin D₃ levels is paralleled by changes in the peripheral blood human T cell compartment.
Khoo AL, Koenen HJ, Chai LY, Sweep FC, Netea MG, van der Ven AJ, Joosten I
PloS one 2012;7(1):e29250
PloS one 2012;7(1):e29250
Impaired function of regulatory T cells in cord blood of children of allergic mothers.
Hrdý J, Kocourková I, Prokešová L
Clinical and experimental immunology 2012 Oct;170(1):10-7
Clinical and experimental immunology 2012 Oct;170(1):10-7
Term and preterm labor: decreased suppressive activity and changes in composition of the regulatory T-cell pool.
Schober L, Radnai D, Schmitt E, Mahnke K, Sohn C, Steinborn A
Immunology and cell biology 2012 Nov;90(10):935-44
Immunology and cell biology 2012 Nov;90(10):935-44
Increased expression of regulatory T cells and down-regulatory molecules in lepromatous leprosy.
Palermo ML, Pagliari C, Trindade MA, Yamashitafuji TM, Duarte AJ, Cacere CR, Benard G
The American journal of tropical medicine and hygiene 2012 May;86(5):878-83
The American journal of tropical medicine and hygiene 2012 May;86(5):878-83
Graft invariant natural killer T-cell dose predicts risk of acute graft-versus-host disease in allogeneic hematopoietic stem cell transplantation.
Chaidos A, Patterson S, Szydlo R, Chaudhry MS, Dazzi F, Kanfer E, McDonald D, Marin D, Milojkovic D, Pavlu J, Davis J, Rahemtulla A, Rezvani K, Goldman J, Roberts I, Apperley J, Karadimitris A
Blood 2012 May 24;119(21):5030-6
Blood 2012 May 24;119(21):5030-6
Immunological parameters associated with the development of allergic rhinitis: a preliminary prospective study.
Uekusa Y, Inamine A, Yonekura S, Horiguchi S, Fujimura T, Sakurai D, Yamamoto H, Suzuki H, Hanazawa T, Okamoto Y
American journal of rhinology & allergy 2012 Mar-Apr;26(2):92-6
American journal of rhinology & allergy 2012 Mar-Apr;26(2):92-6
Induction of endometriosis alters the peripheral and endometrial regulatory T cell population in the non-human primate.
Braundmeier A, Jackson K, Hastings J, Koehler J, Nowak R, Fazleabas A
Human reproduction (Oxford, England) 2012 Jun;27(6):1712-22
Human reproduction (Oxford, England) 2012 Jun;27(6):1712-22
The vaccine-site microenvironment induced by injection of incomplete Freund's adjuvant, with or without melanoma peptides.
Harris RC, Chianese-Bullock KA, Petroni GR, Schaefer JT, Brill LB 2nd, Molhoek KR, Deacon DH, Patterson JW, Slingluff CL Jr
Journal of immunotherapy (Hagerstown, Md. : 1997) 2012 Jan;35(1):78-88
Journal of immunotherapy (Hagerstown, Md. : 1997) 2012 Jan;35(1):78-88
IRX-2, a novel biologic, favors the expansion of T effector over T regulatory cells in a human tumor microenvironment model.
Schilling B, Harasymczuk M, Schuler P, Egan JE, Whiteside TL
Journal of molecular medicine (Berlin, Germany) 2012 Feb;90(2):139-47
Journal of molecular medicine (Berlin, Germany) 2012 Feb;90(2):139-47
Suppression of tumour-specific CD4⁺ T cells by regulatory T cells is associated with progression of human colorectal cancer.
Betts G, Jones E, Junaid S, El-Shanawany T, Scurr M, Mizen P, Kumar M, Jones S, Rees B, Williams G, Gallimore A, Godkin A
Gut 2012 Aug;61(8):1163-71
Gut 2012 Aug;61(8):1163-71
B cell-associated immune profiles in patients with end-stage renal disease (ESRD).
Kim KW, Chung BH, Jeon EJ, Kim BM, Choi BS, Park CW, Kim YS, Cho SG, Cho ML, Yang CW
Experimental & molecular medicine 2012 Aug 31;44(8):465-72
Experimental & molecular medicine 2012 Aug 31;44(8):465-72
OMIP-006: phenotypic subset analysis of human T regulatory cells via polychromatic flow cytometry.
Murdoch DM, Staats JS, Weinhold KJ
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2012 Apr;81(4):281-3
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2012 Apr;81(4):281-3
Choice of resident costimulatory molecule can influence cell fate in human naïve CD4+ T cell differentiation.
Williams KM, Dotson AL, Otto AR, Kohlmeier JE, Benedict SH
Cellular immunology 2011;271(2):418-27
Cellular immunology 2011;271(2):418-27
Engagement of TLR2 reverses the suppressor function of conjunctiva CD4+CD25+ regulatory T cells and promotes herpes simplex virus epitope-specific CD4+CD25- effector T cell responses.
Dasgupta G, Chentoufi AA, You S, Falatoonzadeh P, Urbano LA, Akhtarmalik A, Nguyen K, Ablabutyan L, Nesburn AB, BenMohamed L
Investigative ophthalmology & visual science 2011 May 17;52(6):3321-33
Investigative ophthalmology & visual science 2011 May 17;52(6):3321-33
Separation of human CD4+CD39+ T cells by magnetic beads reveals two phenotypically and functionally different subsets.
Schuler PJ, Harasymczuk M, Schilling B, Lang S, Whiteside TL
Journal of immunological methods 2011 Jun 30;369(1-2):59-68
Journal of immunological methods 2011 Jun 30;369(1-2):59-68
Association between regulatory T cell activity and sepsis and outcome of severely burned patients: a prospective, observational study.
Huang LF, Yao YM, Dong N, Yu Y, He LX, Sheng ZY
Critical care (London, England) 2010;14(1):R3
Critical care (London, England) 2010;14(1):R3
Gender specificity of altered human immune cytokine profiles in aging.
Goetzl EJ, Huang MC, Kon J, Patel K, Schwartz JB, Fast K, Ferrucci L, Madara K, Taub DD, Longo DL
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 Sep;24(9):3580-9
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 Sep;24(9):3580-9
Histone/protein deacetylase inhibitors increase suppressive functions of human FOXP3+ Tregs.
Akimova T, Ge G, Golovina T, Mikheeva T, Wang L, Riley JL, Hancock WW
Clinical immunology (Orlando, Fla.) 2010 Sep;136(3):348-63
Clinical immunology (Orlando, Fla.) 2010 Sep;136(3):348-63
CD4+ CD25+ Foxp3+ regulatory T cells, dendritic cells, and circulating cytokines in uncomplicated malaria: do different parasite species elicit similar host responses?
Gonçalves RM, Salmazi KC, Santos BA, Bastos MS, Rocha SC, Boscardin SB, Silber AM, Kallás EG, Ferreira MU, Scopel KK
Infection and immunity 2010 Nov;78(11):4763-72
Infection and immunity 2010 Nov;78(11):4763-72
Mechanisms of immune suppression for CD8+ T cells by human corneal endothelial cells via membrane-bound TGFbeta.
Yamada Y, Sugita S, Horie S, Yamagami S, Mochizuki M
Investigative ophthalmology & visual science 2010 May;51(5):2548-57
Investigative ophthalmology & visual science 2010 May;51(5):2548-57
Reciprocal granzyme/perforin-mediated death of human regulatory and responder T cells is regulated by interleukin-2 (IL-2).
Czystowska M, Strauss L, Bergmann C, Szajnik M, Rabinowich H, Whiteside TL
Journal of molecular medicine (Berlin, Germany) 2010 Jun;88(6):577-88
Journal of molecular medicine (Berlin, Germany) 2010 Jun;88(6):577-88
CD25 appears non essential for human peripheral T(reg) maintenance in vivo.
de Goër de Herve MG, Gonzales E, Hendel-Chavez H, Décline JL, Mourier O, Abbed K, Jacquemin E, Taoufik Y
PloS one 2010 Jul 30;5(7):e11784
PloS one 2010 Jul 30;5(7):e11784
CD40 signalling induces IL-10-producing, tolerogenic dendritic cells.
Tuettenberg A, Fondel S, Steinbrink K, Enk AH, Jonuleit H
Experimental dermatology 2010 Jan;19(1):44-53
Experimental dermatology 2010 Jan;19(1):44-53
Safety and T cell modulating effects of high dose vitamin D3 supplementation in multiple sclerosis.
Smolders J, Peelen E, Thewissen M, Cohen Tervaert JW, Menheere P, Hupperts R, Damoiseaux J
PloS one 2010 Dec 13;5(12):e15235
PloS one 2010 Dec 13;5(12):e15235
CD4(+) regulatory T cells in a cynomolgus macaque model of Mycobacterium tuberculosis infection.
Green AM, Mattila JT, Bigbee CL, Bongers KS, Lin PL, Flynn JL
The Journal of infectious diseases 2010 Aug 15;202(4):533-41
The Journal of infectious diseases 2010 Aug 15;202(4):533-41
FOXP3+ regulatory T cells and T helper 1, T helper 2, and T helper 17 cells in human early pregnancy decidua.
Mjösberg J, Berg G, Jenmalm MC, Ernerudh J
Biology of reproduction 2010 Apr;82(4):698-705
Biology of reproduction 2010 Apr;82(4):698-705
In colorectal cancer mast cells contribute to systemic regulatory T-cell dysfunction.
Blatner NR, Bonertz A, Beckhove P, Cheon EC, Krantz SB, Strouch M, Weitz J, Koch M, Halverson AL, Bentrem DJ, Khazaie K
Proceedings of the National Academy of Sciences of the United States of America 2010 Apr 6;107(14):6430-5
Proceedings of the National Academy of Sciences of the United States of America 2010 Apr 6;107(14):6430-5
De novo generation and enhanced suppression of human CD4+CD25+ regulatory T cells by retinoic acid.
Wang J, Huizinga TW, Toes RE
Journal of immunology (Baltimore, Md. : 1950) 2009 Sep 15;183(6):4119-26
Journal of immunology (Baltimore, Md. : 1950) 2009 Sep 15;183(6):4119-26
Expansion of allospecific regulatory T cells after anergized, mismatched bone marrow transplantation.
Davies JK, Nadler LM, Guinan EC
Science translational medicine 2009 Oct 7;1(1):1ra3
Science translational medicine 2009 Oct 7;1(1):1ra3
The inhibitory Fc gamma IIb receptor dampens TLR4-mediated immune responses and is selectively up-regulated on dendritic cells from rheumatoid arthritis patients with quiescent disease.
Wenink MH, Santegoets KC, Roelofs MF, Huijbens R, Koenen HJ, van Beek R, Joosten I, Meyer-Wentrup F, Mathsson L, Ronnelid J, Adema GJ, Bonvini E, Koenig S, van den Berg WB, van Riel PL, Radstake TR
Journal of immunology (Baltimore, Md. : 1950) 2009 Oct 1;183(7):4509-20
Journal of immunology (Baltimore, Md. : 1950) 2009 Oct 1;183(7):4509-20
Differential responses of human regulatory T cells (Treg) and effector T cells to rapamycin.
Strauss L, Czystowska M, Szajnik M, Mandapathil M, Whiteside TL
PloS one 2009 Jun 22;4(6):e5994
PloS one 2009 Jun 22;4(6):e5994
Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells.
Nagata S, Ise T, Pastan I
Journal of immunology (Baltimore, Md. : 1950) 2009 Jun 15;182(12):7518-26
Journal of immunology (Baltimore, Md. : 1950) 2009 Jun 15;182(12):7518-26
Vaccine-specific local T cell reactivity in immunotherapy-associated vitiligo in melanoma patients.
Jacobs JF, Aarntzen EH, Sibelt LA, Blokx WA, Boullart AC, Gerritsen MJ, Hoogerbrugge PM, Figdor CG, Adema GJ, Punt CJ, de Vries IJ
Cancer immunology, immunotherapy : CII 2009 Jan;58(1):145-51
Cancer immunology, immunotherapy : CII 2009 Jan;58(1):145-51
The importance of Foxp3 antibody and fixation/permeabilization buffer combinations in identifying CD4+CD25+Foxp3+ regulatory T cells.
Law JP, Hirschkorn DF, Owen RE, Biswas HH, Norris PJ, Lanteri MC
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2009 Dec;75(12):1040-50
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2009 Dec;75(12):1040-50
CD13+CD4+CD25hi regulatory T cells exhibit higher suppressive function and increase with tumor stage in non-small cell lung cancer patients.
Ju S, Qiu H, Zhou X, Zhu B, Lv X, Huang X, Li J, Zhang Y, Liu L, Ge Y, Johnson DE, Ju S, Shu Y
Cell cycle (Georgetown, Tex.) 2009 Aug 15;8(16):2578-85
Cell cycle (Georgetown, Tex.) 2009 Aug 15;8(16):2578-85
Naturally occurring regulatory T cells show reduced sensitivity toward oxidative stress-induced cell death.
Mougiakakos D, Johansson CC, Kiessling R
Blood 2009 Apr 9;113(15):3542-5
Blood 2009 Apr 9;113(15):3542-5
Clinical grade Treg: GMP isolation, improvement of purity by CD127 Depletion, Treg expansion, and Treg cryopreservation.
Peters JH, Preijers FW, Woestenenk R, Hilbrands LB, Koenen HJ, Joosten I
PloS one 2008 Sep 8;3(9):e3161
PloS one 2008 Sep 8;3(9):e3161
Resting regulatory CD4 T cells: a site of HIV persistence in patients on long-term effective antiretroviral therapy.
Tran TA, de Goër de Herve MG, Hendel-Chavez H, Dembele B, Le Névot E, Abbed K, Pallier C, Goujard C, Gasnault J, Delfraissy JF, Balazuc AM, Taoufik Y
PloS one 2008 Oct 1;3(10):e3305
PloS one 2008 Oct 1;3(10):e3305
Expression of ICOS on human melanoma-infiltrating CD4+CD25highFoxp3+ T regulatory cells: implications and impact on tumor-mediated immune suppression.
Strauss L, Bergmann C, Szczepanski MJ, Lang S, Kirkwood JM, Whiteside TL
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):2967-80
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):2967-80
Calcineurin inhibitor withdrawal after renal transplantation with alemtuzumab: clinical outcomes and effect on T-regulatory cells.
Pascual J, Bloom D, Torrealba J, Brahmbhatt R, Chang Z, Sollinger HW, Knechtle SJ
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2008 Jul;8(7):1529-36
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2008 Jul;8(7):1529-36
Immunological monitoring of renal transplant recipients to predict acute allograft rejection following the discontinuation of tacrolimus.
Kreijveld E, Koenen HJ, van Cranenbroek B, van Rijssen E, Joosten I, Hilbrands LB
PloS one 2008 Jul 16;3(7):e2711
PloS one 2008 Jul 16;3(7):e2711
Human regulatory T cells inhibit polarization of T helper cells toward antigen-presenting cells via a TGF-beta-dependent mechanism.
Esquerré M, Tauzin B, Guiraud M, Müller S, Saoudi A, Valitutti S
Proceedings of the National Academy of Sciences of the United States of America 2008 Feb 19;105(7):2550-5
Proceedings of the National Academy of Sciences of the United States of America 2008 Feb 19;105(7):2550-5
Enhanced functionality of CD4+CD25(high)FoxP3+ regulatory T cells in the peripheral blood of patients with prostate cancer.
Yokokawa J, Cereda V, Remondo C, Gulley JL, Arlen PM, Schlom J, Tsang KY
Clinical cancer research : an official journal of the American Association for Cancer Research 2008 Feb 15;14(4):1032-40
Clinical cancer research : an official journal of the American Association for Cancer Research 2008 Feb 15;14(4):1032-40
Safety and immunologic response of a viral vaccine to prostate-specific antigen in combination with radiation therapy when metronomic-dose interleukin 2 is used as an adjuvant.
Lechleider RJ, Arlen PM, Tsang KY, Steinberg SM, Yokokawa J, Cereda V, Camphausen K, Schlom J, Dahut WL, Gulley JL
Clinical cancer research : an official journal of the American Association for Cancer Research 2008 Aug 15;14(16):5284-91
Clinical cancer research : an official journal of the American Association for Cancer Research 2008 Aug 15;14(16):5284-91
Expansion of CD4+CD25+ suppressive regulatory T cells from rhesus macaque peripheral blood by FN18/antihuman CD28-coated Dynal beads.
Gansuvd B, Asiedu CK, Goodwin J, Jargal U, Deckard LA, Andrades P, Guarcello V, Thomas JM
Human immunology 2007 Jun;68(6):478-90
Human immunology 2007 Jun;68(6):478-90
IL-15 and dermal fibroblasts induce proliferation of natural regulatory T cells isolated from human skin.
Clark RA, Kupper TS
Blood 2007 Jan 1;109(1):194-202
Blood 2007 Jan 1;109(1):194-202
FOXP3 regulates TLR10 expression in human T regulatory cells.
Bell MP, Svingen PA, Rahman MK, Xiong Y, Faubion WA Jr
Journal of immunology (Baltimore, Md. : 1950) 2007 Aug 1;179(3):1893-900
Journal of immunology (Baltimore, Md. : 1950) 2007 Aug 1;179(3):1893-900
A novel mechanism of action for anti-thymocyte globulin: induction of CD4+CD25+Foxp3+ regulatory T cells.
Lopez M, Clarkson MR, Albin M, Sayegh MH, Najafian N
Journal of the American Society of Nephrology : JASN 2006 Oct;17(10):2844-53
Journal of the American Society of Nephrology : JASN 2006 Oct;17(10):2844-53
Mucosal but not peripheral FOXP3+ regulatory T cells are highly increased in untreated HIV infection and normalize after suppressive HAART.
Epple HJ, Loddenkemper C, Kunkel D, Tröger H, Maul J, Moos V, Berg E, Ullrich R, Schulzke JD, Stein H, Duchmann R, Zeitz M, Schneider T
Blood 2006 Nov 1;108(9):3072-8
Blood 2006 Nov 1;108(9):3072-8
IL-2 administration increases CD4+ CD25(hi) Foxp3+ regulatory T cells in cancer patients.
Ahmadzadeh M, Rosenberg SA
Blood 2006 Mar 15;107(6):2409-14
Blood 2006 Mar 15;107(6):2409-14
Foxp3+CD4+CD25+ T cells control virus-specific memory T cells in chimpanzees that recovered from hepatitis C.
Manigold T, Shin EC, Mizukoshi E, Mihalik K, Murthy KK, Rice CM, Piccirillo CA, Rehermann B
Blood 2006 Jun 1;107(11):4424-32
Blood 2006 Jun 1;107(11):4424-32
Depletion of alloreactive T cells via CD69: implications on antiviral, antileukemic and immunoregulatory T lymphocytes.
Hartwig UF, Nonn M, Khan S, Meyer RG, Huber C, Herr W
Bone marrow transplantation 2006 Feb;37(3):297-305
Bone marrow transplantation 2006 Feb;37(3):297-305
Rapamycin, and not cyclosporin A, preserves the highly suppressive CD27+ subset of human CD4+CD25+ regulatory T cells.
Coenen JJ, Koenen HJ, van Rijssen E, Hilbrands LB, Joosten I
Blood 2006 Feb 1;107(3):1018-23
Blood 2006 Feb 1;107(3):1018-23
Cutting edge: direct suppression of B cells by CD4+ CD25+ regulatory T cells.
Lim HW, Hillsamer P, Banham AH, Kim CH
Journal of immunology (Baltimore, Md. : 1950) 2005 Oct 1;175(7):4180-3
Journal of immunology (Baltimore, Md. : 1950) 2005 Oct 1;175(7):4180-3
Cutting edge: direct suppression of B cells by CD4+ CD25+ regulatory T cells.
Lim HW, Hillsamer P, Banham AH, Kim CH
Journal of immunology (Baltimore, Md. : 1950) 2005 Oct 1;175(7):4180-3
Journal of immunology (Baltimore, Md. : 1950) 2005 Oct 1;175(7):4180-3
Human CD4+ T cells express TLR5 and its ligand flagellin enhances the suppressive capacity and expression of FOXP3 in CD4+CD25+ T regulatory cells.
Crellin NK, Garcia RV, Hadisfar O, Allan SE, Steiner TS, Levings MK
Journal of immunology (Baltimore, Md. : 1950) 2005 Dec 15;175(12):8051-9
Journal of immunology (Baltimore, Md. : 1950) 2005 Dec 15;175(12):8051-9
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Supportive validation
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- Surface staining of normal human peripheral blood cells with Anti-Human CD4 PE (Product # 12-0049-42) (left) and Anti-Human CD25 APC (Product # 17-0259-42) (right) and subsequently fixed and permeabilized using the Foxp3 Staining Buffers (Product # 00-5523-00) and stained with Anti-Human Foxp3 FITC. Cells in the lymphocyte gate were used for analysis.
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- Figure 1. Flow cytometric mapping of the CD4 + CD25 + Foxp3 + cells in peripheral blood withdrawn from the syphilitic patients with sero-resistance. Foxp3, forkhead box P3.
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- Figure 1 Pre-treatment lymphocyte subpopulations profile of breast cancer and pathological response to neoadjuvant chemotherapy. A) representative digital images (100x) of ductal invasive carcinoma of the breast showing CD3, CD4, CD8, CD20, CD68 and Foxp3 staining (scale bar, 100 mum). B) unsupervised hierarchical clustering analysis of pre-chemotherapy TIL subpopulations yielded three groups or immune clusters (arbitrarily named A, B and C); each column represents a patient and each row represents an immunohistochemical marker. C) pathologic complete response distribution according to pre-chemotherapy immune cluster group. D) differential distribution of lymphocyte populations (CD3, CD4, CD8, CD20, Foxp3, CD68) according to pathological response to neoadjuvant chemotherapy; differences were statistically significant for CD3, CD4 and CD20 and non significant for CD8, Foxp3 and CD68. Statistical analysis: Mann-Whitney U-test. *, P
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- Figure 3 Post-chemotherapy immune cell subpopulation profile and breast cancer prognosis. A) unsupervised hierarchical clustering analysis of post-chemotherapy immune cell populations generated two groups (named as Y and Z); each column represents a patient and each row represents an immunohistochemical marker. B) distribution of immune cell populations in post-chemotherapy cluster groups Y and Z; statistically significant higher infiltration was shown in cluster Y for all populations (CD3, CD4, CD8, CD20, Foxp3, CD68), Mann-Whitney U-test. C) Kaplan-Meier curves showed a worse disease-free survival for patients with tumors belonging to post-chemotherapy immune cluster Y ( P = 0.01). D) disease free survival curves according to post-treatment CD68 infiltration in residual tumor ( P = 0.055). E) Kaplan-Meier disease free survival curves according to level of post-treatment CD3 infiltration in residual tumor, showing an increasingly worse prognosis as CD3 infiltration increases ( P = 0.038). Hazard ratios (HR) and 95%CI calculated according to Cox proportional hazard regression models.
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- Figure 2 Phenotypic analysis of circulating regulatory T cells (Treg). A) Isolated PBMC were analyzed directly ex-vivo by flow cytometry. In the lymphogate, CD3 + CD4 + cells were assessed for the proportions of CD25 + FoxP3 + and CD25 + CD127 - Treg cells. Expression of CD45RA was analyzed to phenotype naive (CD45RA + ) and memory (CD45RA - ) Treg. B-D) The proportions of circulating CD25 + FoxP3 + (B) and CD25 + CD127 - (C)Treg, and of naive Treg (D) before and after vitamin D 3 supplementation (week 0 and 12). Significance was assessed with the Wilcoxon signed ranks comparison test.
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- Figure 3 Naive CD4 + T cells are converted to functional Tregs by tumor-infiltrating DCs and tumor conditioned medium (CM). (A-C) Naive CD4 + T cells from peripheral blood of patients with invasive breast carcinoma were co-cultured with or without autologous pDCs isolated from tumor (TI) or peripheral blood (PB) for 9 days in the presence or absence of 30% CM from autologous tumor slices or adjacent normal tissue slices. (A , B) Non-adherent cells from co-cultures were stained for CD3, CD4, CD25 and intracellular Foxp3, and analyzed by flow cytometry. Representative plots of gated CD3 + CD4 + cells (A) and quantification of percentage of Foxp3 + CD25 + cells among CD3 + CD4 + cells (B) are shown (mean +- SEM, n = 19; * P < 0.05, ** P < 0.01, *** P < 0.001 by Student's t -test). (C) Expression of Treg-associated genes, assessed by qRT-PCR normalized to GAPDH , in sorted CD4 + T cells, relative to expression in cultures without DCs or CM (mean +- SEM, n = 19; * P < 0.05, ** P < 0.01, *** P < 0.001 compared with naive CD4 + T cells cultured alone by Student's t -test). (D-G) Effect of naive CD4 + T cell-derived Tregs, obtained by co-culture with TI pDCs and tumor CM as above, on function of autologous tumor-specific CD8 + T cells. Tumor-specific CD8 + T cells were generated for each subject by stimulating autologous PB CD8 + T cells with autologous tumor lysate-pulsed autologous DCs. Tregs were recovered from co-cultures by magnetic sorting. (D) CFSE-labeled CD8 + T ce
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- Figure 6 In vivo knockdown of PITPNM3 in CD4 + T cells reverses immunosuppression and inhibits tumor progression in humanized mice. (A) Humanized mice bearing palpable MDA-MB-231 orthotopic xenografts were intraperitoneally injected daily for 14 days with PBS, 1 nmol CD4-aptamer-control siRNA (AsiC-con) or CD4-aptamer-siRNA targeting PITPNM3 (sequence in A , AsiC-PI) to assess the role of PITPNM3 in TI Tregs, and other T cells and tumor control. Experimental schematic is provided in Supplementary information, Figure S9A . (B) Representative immunoblots showing selective knockdown of PITPNM3 protein in PB CD4 + T cells, but not tumor xenografts ( n = 3). (C) PITPNM3 knockdown did not affect the distribution of human CD45 + hematopoietic cells, CD4 + and CD8 + T cells, and CD14 + monocytes in the peripheral blood of humanized mice. Representative flow plots are shown ( n = 3). (D , E) Effect of PITPNM3 knockdown on TI naive CD4 + , Tregs and CD8 + T cell numbers, and apoptosis by TUNEL assay in xenografts. D shows representative immunofluorescence microscopy images. Top row indicates CD4 + naive T cells by arrows; the second row indicates CD4 + CD45RO + Foxp3 - CD4 + memory T cells (yellow arrows) and Foxp3 + Tregs (white arrows). Scale bar, 50 mum. E shows number of cells of each subtype/high power field in eight mice ( ** P < 0.01, *** P < 0.001 compared to PBS group by Student's t -test). (F) Flow cytometry analysis of gated human CD3 + CD4 + cells isolated from xenogra
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