12-4777-41
antibody from Invitrogen Antibodies
Targeting: FOXP3
AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID
Antibody data
- Antibody Data
- Antigen structure
- References [78]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [12]
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Validation data
Reference
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- Product number
- 12-4777-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FOXP3 Monoclonal Antibody (236A/E7), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 236A/E7 antibody reacts with human Foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of Foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells. Intracellular staining and flow cytometric analysis of freshly isolated human peripheral blood mononuclear cells (PBMCs) with the 236A/E7 antibody using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523) and protocol reveals staining of the CD4+CD25bright population. The epitope from 236A/E7 is different from that of PCH101 (Product # 72-5776-40). This antibody has also been shown to recognize rhesus macaque, sooty mangabey and cynomolgus macaque. Applications Reported: This 236A/E7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis and IHC. Applications Tested: This 236A/E7 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- 236A/E7
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Tumor-associated tissue eosinophilia promotes angiogenesis and metastasis in head and neck squamous cell carcinoma.
Preclinical assessment of antigen-specific chimeric antigen receptor regulatory T cells for use in solid organ transplantation.
Progressive genetic modifications of porcine cardiac xenografts extend survival to 9 months.
Aberrant newborn T cell and microbiota developmental trajectories predict respiratory compromise during infancy.
Adiponectin/AdipoR1 Axis Promotes IL-10 Release by Human Regulatory T Cells.
Role of the thymus in spontaneous development of a multi-organ autoimmune disease in human immune system mice.
Immunological status of peripheral blood is associated with prognosis in patients with bone and soft-tissue sarcoma.
Changes in expression of PD-L1 on peripheral T cells in patients with melanoma and lung cancer treated with PD-1 inhibitors.
In-Depth Immune-Oncology Studies of the Tumor Microenvironment in a Humanized Melanoma Mouse Model.
Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition).
Redundant cytokine requirement for intestinal microbiota-induced Th17 cell differentiation in draining lymph nodes.
The anti-CXCL4 antibody depletes CD4(+)CD25(+)FOXP3(+) regulatory T cells in CD4+ T cells from chronic osteomyelitis patients by the STAT5 pathway.
Propionic Acid Shapes the Multiple Sclerosis Disease Course by an Immunomodulatory Mechanism.
Mesenchymal Stem and Stromal Cells Harness Macrophage-Derived Amphiregulin to Maintain Tissue Homeostasis.
Accumulation of TNFR2-expressing regulatory T cells in malignant pleural effusion of lung cancer patients is associated with poor prognosis.
Stability and inhibitory function of Treg cells under inflammatory conditions in vitro.
Targeting the mTOR pathway uncouples the efficacy and toxicity of PD-1 blockade in renal transplantation.
Systematic testing and specificity mapping of alloantigen-specific chimeric antigen receptors in regulatory T cells.
Subclinical Cytomegalovirus DNA Is Associated with CD4 T Cell Activation and Impaired CD8 T Cell CD107a Expression in People Living with HIV despite Early Antiretroviral Therapy.
Targeting VEGFR2 with Ramucirumab strongly impacts effector/ activated regulatory T cells and CD8(+) T cells in the tumor microenvironment.
Early expansion of donor-specific Tregs in tolerant kidney transplant recipients.
Cycling CD4+ T cells in HIV-infected immune nonresponders have mitochondrial dysfunction.
Hepatocyte-derived exosomes promote T follicular regulatory cell expansion during hepatitis C virus infection.
FoxP3 isoforms and PD-1 expression by T regulatory cells in multiple sclerosis.
Cytokine-induced alterations of BAMBI mediate the reciprocal regulation of human Th17/Treg cells in response to cigarette smoke extract.
Humoral immune response to adenovirus induce tolerogenic bystander dendritic cells that promote generation of regulatory T cells.
Enhanced IL-6/phosphorylated STAT3 signaling is related to the imbalance of circulating T follicular helper/T follicular regulatory cells in patients with rheumatoid arthritis.
Pancreatic cancer therapy with combined mesothelin-redirected chimeric antigen receptor T cells and cytokine-armed oncolytic adenoviruses.
Tumor-Induced Generation of Splenic Erythroblast-like Ter-Cells Promotes Tumor Progression.
ATP Release from Chemotherapy-Treated Dying Leukemia Cells Elicits an Immune Suppressive Effect by Increasing Regulatory T Cells and Tolerogenic Dendritic Cells.
Tadalafil has biologic activity in human melanoma. Results of a pilot trial with Tadalafil in patients with metastatic Melanoma (TaMe).
Regulatory T Cell Migration Is Dependent on Glucokinase-Mediated Glycolysis.
Oroxylin A inhibits the generation of Tregs in non-small cell lung cancer.
Immune Modifications in Fetal Membranes Overlying the Cervix Precede Parturition in Humans.
Tregitopes and impaired antigen presentation: Drivers of the immunomodulatory effects of IVIg?
HDAC inhibition potentiates immunotherapy in triple negative breast cancer.
Blocking the recruitment of naive CD4(+) T cells reverses immunosuppression in breast cancer.
Imbalance between subsets of CD8(+) peripheral blood T cells in patients with chronic obstructive pulmonary disease.
CD3(+)CD8(+)CD28(-) T Lymphocytes in Patients with Lupus Nephritis.
Differential effects of α4β7 and GPR15 on homing of effector and regulatory T cells from patients with UC to the inflamed gut in vivo.
CD4(+)CD25(+)CD127(-) and CD4(+)CD25(+)Foxp3(+) Regulatory T Cell Subsets in Mediating Autoimmune Reactivity in Systemic Lupus Erythematosus Patients.
Peripheral Blood Regulatory T-Cell and Type 1 Helper T-Cell Population Decrease after Hepatic Artery Embolization.
Anti-TNF drives regulatory T cell expansion by paradoxically promoting membrane TNF-TNF-RII binding in rheumatoid arthritis.
Regulatory T Cell Modulation by CBP/EP300 Bromodomain Inhibition.
Altered expression of miR-92a correlates with Th17 cell frequency in patients with primary biliary cirrhosis.
Early-life compartmentalization of human T cell differentiation and regulatory function in mucosal and lymphoid tissues.
Effects of transcutaneous acupoint electrical stimulation on the imbalance of Th(1), Th(2), Th(17) and T(reg) cells following thoracotomy of patients with lung cancer.
Alloantigen-specific regulatory T cells generated with a chimeric antigen receptor.
Changes in peripheral blood lymphocytes in polycythemia vera and essential thrombocythemia patients treated with pegylated-interferon alpha and correlation with JAK2(V617F) allelic burden.
Follicular regulatory T cells impair follicular T helper cells in HIV and SIV infection.
Inhibition of CD39 enzymatic function at the surface of tumor cells alleviates their immunosuppressive activity.
Restimulation After Cryopreservation and Thawing Preserves the Phenotype and Function of Expanded Baboon Regulatory T Cells.
Porphyromonas gingivalis infection reduces regulatory T cells in infected atherosclerosis patients.
Detailed characterization of tumor infiltrating lymphocytes in two distinct human solid malignancies show phenotypic similarities.
PIM1 kinase phosphorylates the human transcription factor FOXP3 at serine 422 to negatively regulate its activity under inflammation.
Tumor-derived exosomes promote tumor progression and T-cell dysfunction through the regulation of enriched exosomal microRNAs in human nasopharyngeal carcinoma.
High numbers of differentiated effector CD4 T cells are found in patients with cancer and correlate with clinical response after neoadjuvant therapy of breast cancer.
High CCR6/CCR7 expression and Foxp3+ Treg cell number are positively related to the progression of laryngeal squamous cell carcinoma.
BET bromodomain inhibition suppresses TH17-mediated pathology.
The microenvironment of human neuroblastoma supports the activation of tumor-associated T lymphocytes.
HLA Class II tetramers reveal tissue-specific regulatory T cells that suppress T-cell responses in breast carcinoma patients.
Th17/Treg imbalance in patients with liver cystic echinococcosis.
Tissue-infiltrating lymphocytes analysis reveals large modifications of the duodenal "immunological niche" in coeliac disease after gluten-free diet.
Characterization of interleukin-17-producing regulatory T cells in inflamed intestinal mucosa from patients with inflammatory bowel diseases.
Role of gastric epithelial cell-derived transforming growth factor beta in reduced CD4+ T cell proliferation and development of regulatory T cells during Helicobacter pylori infection.
Frequency of circulating Tregs with demethylated FOXP3 intron 1 in melanoma patients receiving tumor vaccines and potentially Treg-depleting agents.
Enhanced suppressive function of regulatory T cells from patients with immune-mediated diseases following successful ex vivo expansion.
CD4(+) regulatory T cells in a cynomolgus macaque model of Mycobacterium tuberculosis infection.
De novo generation and enhanced suppression of human CD4+CD25+ regulatory T cells by retinoic acid.
The CD4(+) T-cell response of melanoma patients to a MAGE-A3 peptide vaccine involves potential regulatory T cells.
FoxP3+ regulatory T cells suppress early stages of granuloma formation but have little impact on sarcoidosis lesions.
Early resolution of acute immune activation and induction of PD-1 in SIV-infected sooty mangabeys distinguishes nonpathogenic from pathogenic infection in rhesus macaques.
Early resolution of acute immune activation and induction of PD-1 in SIV-infected sooty mangabeys distinguishes nonpathogenic from pathogenic infection in rhesus macaques.
IL-2 administration increases CD4+ CD25(hi) Foxp3+ regulatory T cells in cancer patients.
Analysis of FOXP3 protein expression in human CD4+CD25+ regulatory T cells at the single-cell level.
Outcome in Hodgkin's lymphoma can be predicted from the presence of accompanying cytotoxic and regulatory T cells.
FOXP3, a selective marker for a subset of adult T-cell leukaemia/lymphoma.
The expression of the regulatory T cell-specific forkhead box transcription factor FoxP3 is associated with poor prognosis in ovarian cancer.
Lee TL, Chen TH, Kuo YJ, Lan HY, Yang MH, Chu PY
Neoplasia (New York, N.Y.) 2023 Jan;35:100855
Neoplasia (New York, N.Y.) 2023 Jan;35:100855
Preclinical assessment of antigen-specific chimeric antigen receptor regulatory T cells for use in solid organ transplantation.
Proics E, David M, Mojibian M, Speck M, Lounnas-Mourey N, Govehovitch A, Baghdadi W, Desnouveaux J, Bastian H, Freschi L, Privat G, Pouzet C, Grossi M, Heimendinger P, Abel T, Fenard D, Levings MK, Meyer F, Dumont C
Gene therapy 2023 Apr;30(3-4):309-322
Gene therapy 2023 Apr;30(3-4):309-322
Progressive genetic modifications of porcine cardiac xenografts extend survival to 9 months.
Mohiuddin MM, Goerlich CE, Singh AK, Zhang T, Tatarov I, Lewis B, Sentz F, Hershfeld A, Braileanu G, Odonkor P, Strauss E, Williams B, Burke A, Hittman J, Bhutta A, Tabatabai A, Gupta A, Vaught T, Sorrells L, Kuravi K, Dandro A, Eyestone W, Kaczorowski DJ, Ayares D, Griffith BP
Xenotransplantation 2022 May;29(3):e12744
Xenotransplantation 2022 May;29(3):e12744
Aberrant newborn T cell and microbiota developmental trajectories predict respiratory compromise during infancy.
McDavid A, Laniewski N, Grier A, Gill AL, Kessler HA, Huyck H, Carbonell E, Holden-Wiltse J, Bandyopadhyay S, Carnahan J, Dylag AM, Topham DJ, Falsey AR, Caserta MT, Pryhuber GS, Gill SR, Scheible KM
iScience 2022 Apr 15;25(4):104007
iScience 2022 Apr 15;25(4):104007
Adiponectin/AdipoR1 Axis Promotes IL-10 Release by Human Regulatory T Cells.
Ramos-Ramírez P, Malmhäll C, Tliba O, Rådinger M, Bossios A
Frontiers in immunology 2021;12:677550
Frontiers in immunology 2021;12:677550
Role of the thymus in spontaneous development of a multi-organ autoimmune disease in human immune system mice.
Khosravi-Maharlooei M, Li H, Hoelzl M, Zhao G, Ruiz A, Misra A, Li Y, Teteloshvili N, Nauman G, Danzl N, Ding X, Pinker EY, Obradovic A, Yang YG, Iuga A, Creusot RJ, Winchester R, Sykes M
Journal of autoimmunity 2021 May;119:102612
Journal of autoimmunity 2021 May;119:102612
Immunological status of peripheral blood is associated with prognosis in patients with bone and soft-tissue sarcoma.
Kim Y, Kobayashi E, Suehara Y, Ito A, Kubota D, Tanzawa Y, Endo M, Nakatani F, Nakatsura T, Kawai A, Kaneko K, Kitano S
Oncology letters 2021 Mar;21(3):212
Oncology letters 2021 Mar;21(3):212
Changes in expression of PD-L1 on peripheral T cells in patients with melanoma and lung cancer treated with PD-1 inhibitors.
Dart SJ, Cook AM, Millward MJ, McDonnell AM, Chin WL, Hakeem MU, Meniawy TM, Bowyer SE
Scientific reports 2021 Jul 28;11(1):15312
Scientific reports 2021 Jul 28;11(1):15312
In-Depth Immune-Oncology Studies of the Tumor Microenvironment in a Humanized Melanoma Mouse Model.
Schupp J, Christians A, Zimmer N, Gleue L, Jonuleit H, Helm M, Tuettenberg A
International journal of molecular sciences 2021 Jan 20;22(3)
International journal of molecular sciences 2021 Jan 20;22(3)
Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition).
Cossarizza A, Chang HD, Radbruch A, Abrignani S, Addo R, Akdis M, Andrä I, Andreata F, Annunziato F, Arranz E, Bacher P, Bari S, Barnaba V, Barros-Martins J, Baumjohann D, Beccaria CG, Bernardo D, Boardman DA, Borger J, Böttcher C, Brockmann L, Burns M, Busch DH, Cameron G, Cammarata I, Cassotta A, Chang Y, Chirdo FG, Christakou E, Čičin-Šain L, Cook L, Corbett AJ, Cornelis R, Cosmi L, Davey MS, De Biasi S, De Simone G, Del Zotto G, Delacher M, Di Rosa F, Di Santo J, Diefenbach A, Dong J, Dörner T, Dress RJ, Dutertre CA, Eckle SBG, Eede P, Evrard M, Falk CS, Feuerer M, Fillatreau S, Fiz-Lopez A, Follo M, Foulds GA, Fröbel J, Gagliani N, Galletti G, Gangaev A, Garbi N, Garrote JA, Geginat J, Gherardin NA, Gibellini L, Ginhoux F, Godfrey DI, Gruarin P, Haftmann C, Hansmann L, Harpur CM, Hayday AC, Heine G, Hernández DC, Herrmann M, Hoelsken O, Huang Q, Huber S, Huber JE, Huehn J, Hundemer M, Hwang WYK, Iannacone M, Ivison SM, Jäck HM, Jani PK, Keller B, Kessler N, Ketelaars S, Knop L, Knopf J, Koay HF, Kobow K, Kriegsmann K, Kristyanto H, Krueger A, Kuehne JF, Kunze-Schumacher H, Kvistborg P, Kwok I, Latorre D, Lenz D, Levings MK, Lino AC, Liotta F, Long HM, Lugli E, MacDonald KN, Maggi L, Maini MK, Mair F, Manta C, Manz RA, Mashreghi MF, Mazzoni A, McCluskey J, Mei HE, Melchers F, Melzer S, Mielenz D, Monin L, Moretta L, Multhoff G, Muñoz LE, Muñoz-Ruiz M, Muscate F, Natalini A, Neumann K, Ng LG, Niedobitek A, Niemz J, Almeida LN, Notarbartolo S, Ostendorf L, Pallett LJ, Patel AA, Percin GI, Peruzzi G, Pinti M, Pockley AG, Pracht K, Prinz I, Pujol-Autonell I, Pulvirenti N, Quatrini L, Quinn KM, Radbruch H, Rhys H, Rodrigo MB, Romagnani C, Saggau C, Sakaguchi S, Sallusto F, Sanderink L, Sandrock I, Schauer C, Scheffold A, Scherer HU, Schiemann M, Schildberg FA, Schober K, Schoen J, Schuh W, Schüler T, Schulz AR, Schulz S, Schulze J, Simonetti S, Singh J, Sitnik KM, Stark R, Starossom S, Stehle C, Szelinski F, Tan L, Tarnok A, Tornack J, Tree TIM, van Beek JJP, van de Veen W, van Gisbergen K, Vasco C, Verheyden NA, von Borstel A, Ward-Hartstonge KA, Warnatz K, Waskow C, Wiedemann A, Wilharm A, Wing J, Wirz O, Wittner J, Yang JHM, Yang J
European journal of immunology 2021 Dec;51(12):2708-3145
European journal of immunology 2021 Dec;51(12):2708-3145
Redundant cytokine requirement for intestinal microbiota-induced Th17 cell differentiation in draining lymph nodes.
Sano T, Kageyama T, Fang V, Kedmi R, Martinez CS, Talbot J, Chen A, Cabrera I, Gorshko O, Kurakake R, Yang Y, Ng C, Schwab SR, Littman DR
Cell reports 2021 Aug 24;36(8):109608
Cell reports 2021 Aug 24;36(8):109608
The anti-CXCL4 antibody depletes CD4(+)CD25(+)FOXP3(+) regulatory T cells in CD4+ T cells from chronic osteomyelitis patients by the STAT5 pathway.
Huang K, Ge S
Annals of palliative medicine 2020 Sep;9(5):2723-2730
Annals of palliative medicine 2020 Sep;9(5):2723-2730
Propionic Acid Shapes the Multiple Sclerosis Disease Course by an Immunomodulatory Mechanism.
Duscha A, Gisevius B, Hirschberg S, Yissachar N, Stangl GI, Dawin E, Bader V, Haase S, Kaisler J, David C, Schneider R, Troisi R, Zent D, Hegelmaier T, Dokalis N, Gerstein S, Del Mare-Roumani S, Amidror S, Staszewski O, Poschmann G, Stühler K, Hirche F, Balogh A, Kempa S, Träger P, Zaiss MM, Holm JB, Massa MG, Nielsen HB, Faissner A, Lukas C, Gatermann SG, Scholz M, Przuntek H, Prinz M, Forslund SK, Winklhofer KF, Müller DN, Linker RA, Gold R, Haghikia A
Cell 2020 Mar 19;180(6):1067-1080.e16
Cell 2020 Mar 19;180(6):1067-1080.e16
Mesenchymal Stem and Stromal Cells Harness Macrophage-Derived Amphiregulin to Maintain Tissue Homeostasis.
Ko JH, Kim HJ, Jeong HJ, Lee HJ, Oh JY
Cell reports 2020 Mar 17;30(11):3806-3820.e6
Cell reports 2020 Mar 17;30(11):3806-3820.e6
Accumulation of TNFR2-expressing regulatory T cells in malignant pleural effusion of lung cancer patients is associated with poor prognosis.
Ye LL, Peng WB, Niu YR, Xiang X, Wei XS, Wang ZH, Wang X, Zhang SY, Chen X, Zhou Q
Annals of translational medicine 2020 Dec;8(24):1647
Annals of translational medicine 2020 Dec;8(24):1647
Stability and inhibitory function of Treg cells under inflammatory conditions in vitro.
Guo H, Xun L, Zhang R, Hu F, Luan J, Lao K, Wang X, Gou X
Experimental and therapeutic medicine 2019 Oct;18(4):2443-2450
Experimental and therapeutic medicine 2019 Oct;18(4):2443-2450
Targeting the mTOR pathway uncouples the efficacy and toxicity of PD-1 blockade in renal transplantation.
Esfahani K, Al-Aubodah TA, Thebault P, Lapointe R, Hudson M, Johnson NA, Baran D, Bhulaiga N, Takano T, Cailhier JF, Piccirillo CA, Miller WH
Nature communications 2019 Oct 17;10(1):4712
Nature communications 2019 Oct 17;10(1):4712
Systematic testing and specificity mapping of alloantigen-specific chimeric antigen receptors in regulatory T cells.
Dawson NA, Lamarche C, Hoeppli RE, Bergqvist P, Fung VC, McIver E, Huang Q, Gillies J, Speck M, Orban PC, Bush JW, Mojibian M, Levings MK
JCI insight 2019 Mar 21;4(6)
JCI insight 2019 Mar 21;4(6)
Subclinical Cytomegalovirus DNA Is Associated with CD4 T Cell Activation and Impaired CD8 T Cell CD107a Expression in People Living with HIV despite Early Antiretroviral Therapy.
Christensen-Quick A, Massanella M, Frick A, Rawlings SA, Spina C, Vargas-Meneses M, Schrier R, Nakazawa M, Anderson C, Gianella S
Journal of virology 2019 Jul 1;93(13)
Journal of virology 2019 Jul 1;93(13)
Targeting VEGFR2 with Ramucirumab strongly impacts effector/ activated regulatory T cells and CD8(+) T cells in the tumor microenvironment.
Tada Y, Togashi Y, Kotani D, Kuwata T, Sato E, Kawazoe A, Doi T, Wada H, Nishikawa H, Shitara K
Journal for immunotherapy of cancer 2018 Oct 11;6(1):106
Journal for immunotherapy of cancer 2018 Oct 11;6(1):106
Early expansion of donor-specific Tregs in tolerant kidney transplant recipients.
Savage TM, Shonts BA, Obradovic A, Dewolf S, Lau S, Zuber J, Simpson MT, Berglund E, Fu J, Yang S, Ho SH, Tang Q, Turka LA, Shen Y, Sykes M
JCI insight 2018 Nov 15;3(22)
JCI insight 2018 Nov 15;3(22)
Cycling CD4+ T cells in HIV-infected immune nonresponders have mitochondrial dysfunction.
Younes SA, Talla A, Pereira Ribeiro S, Saidakova EV, Korolevskaya LB, Shmagel KV, Shive CL, Freeman ML, Panigrahi S, Zweig S, Balderas R, Margolis L, Douek DC, Anthony DD, Pandiyan P, Cameron M, Sieg SF, Calabrese LH, Rodriguez B, Lederman MM
The Journal of clinical investigation 2018 Nov 1;128(11):5083-5094
The Journal of clinical investigation 2018 Nov 1;128(11):5083-5094
Hepatocyte-derived exosomes promote T follicular regulatory cell expansion during hepatitis C virus infection.
Cobb DA, Kim OK, Golden-Mason L, Rosen HR, Hahn YS
Hepatology (Baltimore, Md.) 2018 Jan;67(1):71-85
Hepatology (Baltimore, Md.) 2018 Jan;67(1):71-85
FoxP3 isoforms and PD-1 expression by T regulatory cells in multiple sclerosis.
Sambucci M, Gargano F, De Rosa V, De Bardi M, Picozza M, Placido R, Ruggieri S, Capone A, Gasperini C, Matarese G, Battistini L, Borsellino G
Scientific reports 2018 Feb 27;8(1):3674
Scientific reports 2018 Feb 27;8(1):3674
Cytokine-induced alterations of BAMBI mediate the reciprocal regulation of human Th17/Treg cells in response to cigarette smoke extract.
Liu HJ, Chen G, Chen L, Zhou M, Xiong XZ, Meng ZJ, Sun SW, Tao XN
International journal of molecular medicine 2018 Dec;42(6):3404-3414
International journal of molecular medicine 2018 Dec;42(6):3404-3414
Humoral immune response to adenovirus induce tolerogenic bystander dendritic cells that promote generation of regulatory T cells.
Tran TTP, Eichholz K, Amelio P, Moyer C, Nemerow GR, Perreau M, Mennechet FJD, Kremer EJ
PLoS pathogens 2018 Aug;14(8):e1007127
PLoS pathogens 2018 Aug;14(8):e1007127
Enhanced IL-6/phosphorylated STAT3 signaling is related to the imbalance of circulating T follicular helper/T follicular regulatory cells in patients with rheumatoid arthritis.
Niu Q, Huang ZC, Wu XJ, Jin YX, An YF, Li YM, Xu H, Yang B, Wang LL
Arthritis research & therapy 2018 Aug 29;20(1):200
Arthritis research & therapy 2018 Aug 29;20(1):200
Pancreatic cancer therapy with combined mesothelin-redirected chimeric antigen receptor T cells and cytokine-armed oncolytic adenoviruses.
Watanabe K, Luo Y, Da T, Guedan S, Ruella M, Scholler J, Keith B, Young RM, Engels B, Sorsa S, Siurala M, Havunen R, Tähtinen S, Hemminki A, June CH
JCI insight 2018 Apr 5;3(7)
JCI insight 2018 Apr 5;3(7)
Tumor-Induced Generation of Splenic Erythroblast-like Ter-Cells Promotes Tumor Progression.
Han Y, Liu Q, Hou J, Gu Y, Zhang Y, Chen Z, Fan J, Zhou W, Qiu S, Zhang Y, Dong T, Li N, Jiang Z, Zhu H, Zhang Q, Ma Y, Zhang L, Wang Q, Yu Y, Li N, Cao X
Cell 2018 Apr 19;173(3):634-648.e12
Cell 2018 Apr 19;173(3):634-648.e12
ATP Release from Chemotherapy-Treated Dying Leukemia Cells Elicits an Immune Suppressive Effect by Increasing Regulatory T Cells and Tolerogenic Dendritic Cells.
Lecciso M, Ocadlikova D, Sangaletti S, Trabanelli S, De Marchi E, Orioli E, Pegoraro A, Portararo P, Jandus C, Bontadini A, Redavid A, Salvestrini V, Romero P, Colombo MP, Di Virgilio F, Cavo M, Adinolfi E, Curti A
Frontiers in immunology 2017;8:1918
Frontiers in immunology 2017;8:1918
Tadalafil has biologic activity in human melanoma. Results of a pilot trial with Tadalafil in patients with metastatic Melanoma (TaMe).
Hassel JC, Jiang H, Bender C, Winkler J, Sevko A, Shevchenko I, Halama N, Dimitrakopoulou-Strauss A, Haefeli WE, Jäger D, Enk A, Utikal J, Umansky V
Oncoimmunology 2017;6(9):e1326440
Oncoimmunology 2017;6(9):e1326440
Regulatory T Cell Migration Is Dependent on Glucokinase-Mediated Glycolysis.
Kishore M, Cheung KCP, Fu H, Bonacina F, Wang G, Coe D, Ward EJ, Colamatteo A, Jangani M, Baragetti A, Matarese G, Smith DM, Haas R, Mauro C, Wraith DC, Okkenhaug K, Catapano AL, De Rosa V, Norata GD, Marelli-Berg FM
Immunity 2017 Nov 21;47(5):875-889.e10
Immunity 2017 Nov 21;47(5):875-889.e10
Oroxylin A inhibits the generation of Tregs in non-small cell lung cancer.
Shen L, Zhang LL, Li H, Liu X, Yu XX, Hu P, Hui H, Guo QL, Zhang S
Oncotarget 2017 Jul 25;8(30):49395-49408
Oncotarget 2017 Jul 25;8(30):49395-49408
Immune Modifications in Fetal Membranes Overlying the Cervix Precede Parturition in Humans.
Marcellin L, Schmitz T, Messaoudene M, Chader D, Parizot C, Jacques S, Delaire J, Gogusev J, Schmitt A, Lesaffre C, Breuiller-Fouché M, Caignard A, Vaiman D, Goffinet F, Cabrol D, Gorochov G, Méhats C
Journal of immunology (Baltimore, Md. : 1950) 2017 Feb 1;198(3):1345-1356
Journal of immunology (Baltimore, Md. : 1950) 2017 Feb 1;198(3):1345-1356
Tregitopes and impaired antigen presentation: Drivers of the immunomodulatory effects of IVIg?
Sordé L, Spindeldreher S, Palmer E, Karle A
Immunity, inflammation and disease 2017 Dec;5(4):400-415
Immunity, inflammation and disease 2017 Dec;5(4):400-415
HDAC inhibition potentiates immunotherapy in triple negative breast cancer.
Terranova-Barberio M, Thomas S, Ali N, Pawlowska N, Park J, Krings G, Rosenblum MD, Budillon A, Munster PN
Oncotarget 2017 Dec 26;8(69):114156-114172
Oncotarget 2017 Dec 26;8(69):114156-114172
Blocking the recruitment of naive CD4(+) T cells reverses immunosuppression in breast cancer.
Su S, Liao J, Liu J, Huang D, He C, Chen F, Yang L, Wu W, Chen J, Lin L, Zeng Y, Ouyang N, Cui X, Yao H, Su F, Huang JD, Lieberman J, Liu Q, Song E
Cell research 2017 Apr;27(4):461-482
Cell research 2017 Apr;27(4):461-482
Imbalance between subsets of CD8(+) peripheral blood T cells in patients with chronic obstructive pulmonary disease.
Chen L, Chen G, Zhang MQ, Xiong XZ, Liu HJ, Xin JB, Zhang JC, Wu JH, Meng ZJ, Sun SW
PeerJ 2016;4:e2301
PeerJ 2016;4:e2301
CD3(+)CD8(+)CD28(-) T Lymphocytes in Patients with Lupus Nephritis.
Żabińska M, Krajewska M, Kościelska-Kasprzak K, Klinger M
Journal of immunology research 2016;2016:1058165
Journal of immunology research 2016;2016:1058165
Differential effects of α4β7 and GPR15 on homing of effector and regulatory T cells from patients with UC to the inflamed gut in vivo.
Fischer A, Zundler S, Atreya R, Rath T, Voskens C, Hirschmann S, López-Posadas R, Watson A, Becker C, Schuler G, Neufert C, Atreya I, Neurath MF
Gut 2016 Oct;65(10):1642-64
Gut 2016 Oct;65(10):1642-64
CD4(+)CD25(+)CD127(-) and CD4(+)CD25(+)Foxp3(+) Regulatory T Cell Subsets in Mediating Autoimmune Reactivity in Systemic Lupus Erythematosus Patients.
Żabińska M, Krajewska M, Kościelska-Kasprzak K, Jakuszko K, Bartoszek D, Myszka M, Klinger M
Archivum immunologiae et therapiae experimentalis 2016 Oct;64(5):399-407
Archivum immunologiae et therapiae experimentalis 2016 Oct;64(5):399-407
Peripheral Blood Regulatory T-Cell and Type 1 Helper T-Cell Population Decrease after Hepatic Artery Embolization.
Takaki H, Imai N, Contessa TT, Srimathveeravalli G, Covey AM, Getrajdman GI, Brown KT, Solomon SB, Erinjeri JP
Journal of vascular and interventional radiology : JVIR 2016 Oct;27(10):1561-8
Journal of vascular and interventional radiology : JVIR 2016 Oct;27(10):1561-8
Anti-TNF drives regulatory T cell expansion by paradoxically promoting membrane TNF-TNF-RII binding in rheumatoid arthritis.
Nguyen DX, Ehrenstein MR
The Journal of experimental medicine 2016 Jun 27;213(7):1241-53
The Journal of experimental medicine 2016 Jun 27;213(7):1241-53
Regulatory T Cell Modulation by CBP/EP300 Bromodomain Inhibition.
Ghosh S, Taylor A, Chin M, Huang HR, Conery AR, Mertz JA, Salmeron A, Dakle PJ, Mele D, Cote A, Jayaram H, Setser JW, Poy F, Hatzivassiliou G, DeAlmeida-Nagata D, Sandy P, Hatton C, Romero FA, Chiang E, Reimer T, Crawford T, Pardo E, Watson VG, Tsui V, Cochran AG, Zawadzke L, Harmange JC, Audia JE, Bryant BM, Cummings RT, Magnuson SR, Grogan JL, Bellon SF, Albrecht BK, Sims RJ 3rd, Lora JM
The Journal of biological chemistry 2016 Jun 17;291(25):13014-27
The Journal of biological chemistry 2016 Jun 17;291(25):13014-27
Altered expression of miR-92a correlates with Th17 cell frequency in patients with primary biliary cirrhosis.
Liang DY, Hou YQ, Luo LJ, Ao L
International journal of molecular medicine 2016 Jul;38(1):131-8
International journal of molecular medicine 2016 Jul;38(1):131-8
Early-life compartmentalization of human T cell differentiation and regulatory function in mucosal and lymphoid tissues.
Thome JJ, Bickham KL, Ohmura Y, Kubota M, Matsuoka N, Gordon C, Granot T, Griesemer A, Lerner H, Kato T, Farber DL
Nature medicine 2016 Jan;22(1):72-7
Nature medicine 2016 Jan;22(1):72-7
Effects of transcutaneous acupoint electrical stimulation on the imbalance of Th(1), Th(2), Th(17) and T(reg) cells following thoracotomy of patients with lung cancer.
Wu H, Wang K, Li G, Meng D, Han J, Wang G, Li YU
Experimental and therapeutic medicine 2016 Feb;11(2):495-502
Experimental and therapeutic medicine 2016 Feb;11(2):495-502
Alloantigen-specific regulatory T cells generated with a chimeric antigen receptor.
MacDonald KG, Hoeppli RE, Huang Q, Gillies J, Luciani DS, Orban PC, Broady R, Levings MK
The Journal of clinical investigation 2016 Apr 1;126(4):1413-24
The Journal of clinical investigation 2016 Apr 1;126(4):1413-24
Changes in peripheral blood lymphocytes in polycythemia vera and essential thrombocythemia patients treated with pegylated-interferon alpha and correlation with JAK2(V617F) allelic burden.
Kovacsovics-Bankowski M, Kelley TW, Efimova O, Kim SJ, Wilson A, Swierczek S, Prchal J
Experimental hematology & oncology 2015;5:28
Experimental hematology & oncology 2015;5:28
Follicular regulatory T cells impair follicular T helper cells in HIV and SIV infection.
Miles B, Miller SM, Folkvord JM, Kimball A, Chamanian M, Meditz AL, Arends T, McCarter MD, Levy DN, Rakasz EG, Skinner PJ, Connick E
Nature communications 2015 Oct 20;6:8608
Nature communications 2015 Oct 20;6:8608
Inhibition of CD39 enzymatic function at the surface of tumor cells alleviates their immunosuppressive activity.
Bastid J, Regairaz A, Bonnefoy N, Déjou C, Giustiniani J, Laheurte C, Cochaud S, Laprevotte E, Funck-Brentano E, Hemon P, Gros L, Bec N, Larroque C, Alberici G, Bensussan A, Eliaou JF
Cancer immunology research 2015 Mar;3(3):254-65
Cancer immunology research 2015 Mar;3(3):254-65
Restimulation After Cryopreservation and Thawing Preserves the Phenotype and Function of Expanded Baboon Regulatory T Cells.
Weiner J, Duran-Struuck R, Zitsman J, Buhler L, Sondermeijer H, McMurchy AN, Levings MK, Sykes M, Griesemer A
Transplantation direct 2015 Feb 1;1(1):1-7
Transplantation direct 2015 Feb 1;1(1):1-7
Porphyromonas gingivalis infection reduces regulatory T cells in infected atherosclerosis patients.
Yang J, Wu J, Liu Y, Huang J, Lu Z, Xie L, Sun W, Ji Y
PloS one 2014;9(1):e86599
PloS one 2014;9(1):e86599
Detailed characterization of tumor infiltrating lymphocytes in two distinct human solid malignancies show phenotypic similarities.
Kovacsovics-Bankowski M, Chisholm L, Vercellini J, Tucker CG, Montler R, Haley D, Newell P, Ma J, Tseng P, Wolf R, Vetto JT, Hammill C, Hansen P, Weinberg AD
Journal for immunotherapy of cancer 2014;2(1):38
Journal for immunotherapy of cancer 2014;2(1):38
PIM1 kinase phosphorylates the human transcription factor FOXP3 at serine 422 to negatively regulate its activity under inflammation.
Li Z, Lin F, Zhuo C, Deng G, Chen Z, Yin S, Gao Z, Piccioni M, Tsun A, Cai S, Zheng SG, Zhang Y, Li B
The Journal of biological chemistry 2014 Sep 26;289(39):26872-26881
The Journal of biological chemistry 2014 Sep 26;289(39):26872-26881
Tumor-derived exosomes promote tumor progression and T-cell dysfunction through the regulation of enriched exosomal microRNAs in human nasopharyngeal carcinoma.
Ye SB, Li ZL, Luo DH, Huang BJ, Chen YS, Zhang XS, Cui J, Zeng YX, Li J
Oncotarget 2014 Jul 30;5(14):5439-52
Oncotarget 2014 Jul 30;5(14):5439-52
High numbers of differentiated effector CD4 T cells are found in patients with cancer and correlate with clinical response after neoadjuvant therapy of breast cancer.
Péguillet I, Milder M, Louis D, Vincent-Salomon A, Dorval T, Piperno-Neumann S, Scholl SM, Lantz O
Cancer research 2014 Apr 15;74(8):2204-16
Cancer research 2014 Apr 15;74(8):2204-16
High CCR6/CCR7 expression and Foxp3+ Treg cell number are positively related to the progression of laryngeal squamous cell carcinoma.
Chen B, Zhang D, Zhou J, Li Q, Zhou L, Li SM, Zhu L, Chou KY, Zhou L, Tao L, Lu LM
Oncology reports 2013 Sep;30(3):1380-90
Oncology reports 2013 Sep;30(3):1380-90
BET bromodomain inhibition suppresses TH17-mediated pathology.
Mele DA, Salmeron A, Ghosh S, Huang HR, Bryant BM, Lora JM
The Journal of experimental medicine 2013 Oct 21;210(11):2181-90
The Journal of experimental medicine 2013 Oct 21;210(11):2181-90
The microenvironment of human neuroblastoma supports the activation of tumor-associated T lymphocytes.
Carlson LM, De Geer A, Sveinbjørnsson B, Orrego A, Martinsson T, Kogner P, Levitskaya J
Oncoimmunology 2013 Mar 1;2(3):e23618
Oncoimmunology 2013 Mar 1;2(3):e23618
HLA Class II tetramers reveal tissue-specific regulatory T cells that suppress T-cell responses in breast carcinoma patients.
Schmidt HH, Ge Y, Hartmann FJ, Conrad H, Klug F, Nittel S, Bernhard H, Domschke C, Schuetz F, Sohn C, Beckhove P
Oncoimmunology 2013 Jun 1;2(6):e24962
Oncoimmunology 2013 Jun 1;2(6):e24962
Th17/Treg imbalance in patients with liver cystic echinococcosis.
Tuxun T, Wang JH, Lin RY, Shan JY, Tai QW, Li T, Zhang JH, Zhao JM, Wen H
Parasite immunology 2012 Nov;34(11):520-7
Parasite immunology 2012 Nov;34(11):520-7
Tissue-infiltrating lymphocytes analysis reveals large modifications of the duodenal "immunological niche" in coeliac disease after gluten-free diet.
Cianci R, Cammarota G, Frisullo G, Pagliari D, Ianiro G, Martini M, Frosali S, Plantone D, Damato V, Casciano F, Landolfi R, Paola Batocchi A, Pandolfi F
Clinical and translational gastroenterology 2012 Dec 13;3(12):e28
Clinical and translational gastroenterology 2012 Dec 13;3(12):e28
Characterization of interleukin-17-producing regulatory T cells in inflamed intestinal mucosa from patients with inflammatory bowel diseases.
Hovhannisyan Z, Treatman J, Littman DR, Mayer L
Gastroenterology 2011 Mar;140(3):957-65
Gastroenterology 2011 Mar;140(3):957-65
Role of gastric epithelial cell-derived transforming growth factor beta in reduced CD4+ T cell proliferation and development of regulatory T cells during Helicobacter pylori infection.
Beswick EJ, Pinchuk IV, Earley RB, Schmitt DA, Reyes VE
Infection and immunity 2011 Jul;79(7):2737-45
Infection and immunity 2011 Jul;79(7):2737-45
Frequency of circulating Tregs with demethylated FOXP3 intron 1 in melanoma patients receiving tumor vaccines and potentially Treg-depleting agents.
de Vries IJ, Castelli C, Huygens C, Jacobs JF, Stockis J, Schuler-Thurner B, Adema GJ, Punt CJ, Rivoltini L, Schuler G, Coulie PG, Lucas S
Clinical cancer research : an official journal of the American Association for Cancer Research 2011 Feb 15;17(4):841-8
Clinical cancer research : an official journal of the American Association for Cancer Research 2011 Feb 15;17(4):841-8
Enhanced suppressive function of regulatory T cells from patients with immune-mediated diseases following successful ex vivo expansion.
Cao T, Wenzel SE, Faubion WA, Harriman G, Li L
Clinical immunology (Orlando, Fla.) 2010 Sep;136(3):329-37
Clinical immunology (Orlando, Fla.) 2010 Sep;136(3):329-37
CD4(+) regulatory T cells in a cynomolgus macaque model of Mycobacterium tuberculosis infection.
Green AM, Mattila JT, Bigbee CL, Bongers KS, Lin PL, Flynn JL
The Journal of infectious diseases 2010 Aug 15;202(4):533-41
The Journal of infectious diseases 2010 Aug 15;202(4):533-41
De novo generation and enhanced suppression of human CD4+CD25+ regulatory T cells by retinoic acid.
Wang J, Huizinga TW, Toes RE
Journal of immunology (Baltimore, Md. : 1950) 2009 Sep 15;183(6):4119-26
Journal of immunology (Baltimore, Md. : 1950) 2009 Sep 15;183(6):4119-26
The CD4(+) T-cell response of melanoma patients to a MAGE-A3 peptide vaccine involves potential regulatory T cells.
François V, Ottaviani S, Renkvist N, Stockis J, Schuler G, Thielemans K, Colau D, Marchand M, Boon T, Lucas S, van der Bruggen P
Cancer research 2009 May 15;69(10):4335-45
Cancer research 2009 May 15;69(10):4335-45
FoxP3+ regulatory T cells suppress early stages of granuloma formation but have little impact on sarcoidosis lesions.
Taflin C, Miyara M, Nochy D, Valeyre D, Naccache JM, Altare F, Salek-Peyron P, Badoual C, Bruneval P, Haroche J, Mathian A, Amoura Z, Hill G, Gorochov G
The American journal of pathology 2009 Feb;174(2):497-508
The American journal of pathology 2009 Feb;174(2):497-508
Early resolution of acute immune activation and induction of PD-1 in SIV-infected sooty mangabeys distinguishes nonpathogenic from pathogenic infection in rhesus macaques.
Estes JD, Gordon SN, Zeng M, Chahroudi AM, Dunham RM, Staprans SI, Reilly CS, Silvestri G, Haase AT
Journal of immunology (Baltimore, Md. : 1950) 2008 May 15;180(10):6798-807
Journal of immunology (Baltimore, Md. : 1950) 2008 May 15;180(10):6798-807
Early resolution of acute immune activation and induction of PD-1 in SIV-infected sooty mangabeys distinguishes nonpathogenic from pathogenic infection in rhesus macaques.
Estes JD, Gordon SN, Zeng M, Chahroudi AM, Dunham RM, Staprans SI, Reilly CS, Silvestri G, Haase AT
Journal of immunology (Baltimore, Md. : 1950) 2008 May 15;180(10):6798-807
Journal of immunology (Baltimore, Md. : 1950) 2008 May 15;180(10):6798-807
IL-2 administration increases CD4+ CD25(hi) Foxp3+ regulatory T cells in cancer patients.
Ahmadzadeh M, Rosenberg SA
Blood 2006 Mar 15;107(6):2409-14
Blood 2006 Mar 15;107(6):2409-14
Analysis of FOXP3 protein expression in human CD4+CD25+ regulatory T cells at the single-cell level.
Roncador G, Brown PJ, Maestre L, Hue S, Martínez-Torrecuadrada JL, Ling KL, Pratap S, Toms C, Fox BC, Cerundolo V, Powrie F, Banham AH
European journal of immunology 2005 Jun;35(6):1681-91
European journal of immunology 2005 Jun;35(6):1681-91
Outcome in Hodgkin's lymphoma can be predicted from the presence of accompanying cytotoxic and regulatory T cells.
Alvaro T, Lejeune M, Salvadó MT, Bosch R, García JF, Jaén J, Banham AH, Roncador G, Montalbán C, Piris MA
Clinical cancer research : an official journal of the American Association for Cancer Research 2005 Feb 15;11(4):1467-73
Clinical cancer research : an official journal of the American Association for Cancer Research 2005 Feb 15;11(4):1467-73
FOXP3, a selective marker for a subset of adult T-cell leukaemia/lymphoma.
Roncador G, Garcia JF, Garcia JF, Maestre L, Lucas E, Menarguez J, Ohshima K, Nakamura S, Banham AH, Piris MA
Leukemia 2005 Dec;19(12):2247-53
Leukemia 2005 Dec;19(12):2247-53
The expression of the regulatory T cell-specific forkhead box transcription factor FoxP3 is associated with poor prognosis in ovarian cancer.
Wolf D, Wolf AM, Rumpold H, Fiegl H, Zeimet AG, Muller-Holzner E, Deibl M, Gastl G, Gunsilius E, Marth C
Clinical cancer research : an official journal of the American Association for Cancer Research 2005 Dec 1;11(23):8326-31
Clinical cancer research : an official journal of the American Association for Cancer Research 2005 Dec 1;11(23):8326-31
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- Staining of normal human peripheral blood cells with Anti-Human CD4 FITC (Product # 11-0049-42) (left) and Anti-Human CD25 APC (Product # 17-0259-42) (right) followed by fixation and permeabilization using the Foxp3 Staining/Transcription Factor Buffer Set (Product # 00-5523-00) and intracellular staining with Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) or Anti-Human Foxp3 PE. Cells in the lymphocyte gate were used for analysis.
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- Figure 3. Tetramer staining of conventional and regulatory T cells from breast cancer patients. ( A-G ) Peripheral blood mononuclear cells (PBMCs) of a breast cancer patient were analyzed, upon gating on living CD3 + CD4 + T cells. The patient sample was stained with tetramers presenting either mam 34-48 ( B ) or the CLIP peptide ( C ). Numbers indicate the percentage of cells in the respective gate, referring to lymphocytes ( A ) or CD3 + CD4 + T cells ( B ) and ( C ). Within CD3 + CD4 + T cells, regulatory T cells (Tregs) were identified as CD25 high CD127 low ( D ) and ( G ) and CD25 high CD127 low FOXP3 + ( E ), ( F ) and ( H ). ( G ) reports the percentage of Tregs within tetramer-positive cells in a representative patient. ( I ) Frequency of mam 34-48 - and CLIP-presenting tetramer-positive cells among CD3 + CD4 + T cells of breast cancer patients and healthy donors (HD) (p values as per Mann-Whitney U tests are indicated). ( J and K ) Frequency of mam 34-48 -specific Tcon ( J ) cells and Tregs ( K ) of breast cancer patients and HDs.
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- Fig. 1 PegINFalpha increases Treg and highly suppressive Treg cells in peripheral blood of PV and ET patients. PBMC were collected from patients with PV or ET treated with PegINFalpha or HU for at least 70 days (range 70-616, median 112 for PegINFalpha and range 113-2422, median 175 for HU treated patients). Lymphocytes were analyzed by flow cytometry using surface markers, CD3, CD4, CD25, CD39, HLA-DR and intracellular markers Foxp3, Ki-67 and Helios. a represents CD4 + CD25 + Foxp3 + Treg cells. In b the frequency of Treg was analyzed at different time points after initiation of PegINFalpha treatment. c-e show frequency of Ki-67 + Treg, Helios + Treg, and CD39 + /HLA-DR + ) Treg
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- Figure 3 NPC tumor-released exosomes inhibited Th1 and Th17 cell induction but promoted Tregs by altering STAT protein phosphorylation A. Differentiation of naive CD4 + T cells into Th1 and Th17 cells and Tregs after stimulation with tumor cells with or without EXOs. Purified naive CD4 + T cells from healthy donors were co-cultured with the irradiated NPC cell line TW03 (EBV + ) and then treated with EXOs or left or untreated in the presence of OKT3 in IL-2 medium for 5 days. INFgamma, IL-17, and Foxp3 staining following by FACS analysis was performed after stimulation with PMA/ionomycin. Representative data of five experiments are shown. B. Numerous data showing the mean percentage +- S.E.M. of positive cell subsets in three independent experiments. * means P < 0.05. C. Western blot analysis for the expression of P-STAT1, P-STAT3, and P-STAT5 proteins. Stimulation with EXOs decreased the expression of P-STAT1 and P-STAT3 but increased the expression of P-STAT5 in OKT3 and NPC cells stimulated by CD4 + T cells; the GAPDH gene was included as a control. Representative data of three experiments are shown.
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- Figure 3 HIV entry and replication promote T FR expansion. Disaggregated tonsil cells were spinoculated with X4 or R5 HIV and T FR populations were analysed by flow cytometry ( n =15). ( a ) A representative example of tonsil cell flow gating. From viable CD3 + CD8 - cells, T FR are defined as CXCR5 + and CD25 hi CD127 - . T FR cells contain Foxp3 + cells, whereas remaining T FH (CXCR5 + CD25 lo/- ) cells are Foxp3 - . ( b ) Percentages of T FR determined by gating strategies in a are shown. Experimental conditions include mock-spinoculated cells cultured with PMA (50 ng ml -1 ) and ionomycin (1 mug ml -1 ) or exogenous TGF-beta (100 ng ml -1 ) for 24 h and cells pretreated to block CXCR4 (AMD, 200 muM) and CCR5 (MVC, 2 muM). ( c ) Using flow cytometry counting beads, the number of cells per mul were determined for total (CD3 + CD8 - ), T FH (CXCR5 + CD25 lo/- ) and T FR (CXCR5 + CD25 hi CD127 - ) subsets in mock- and X4-spinoculated samples ( n =3). ( d ) Bcl-6 expression is shown in CXCR5- (grey), T FH (blue) and T FR (red) populations after mock-, X4- or R5-spinoculation ( n =5). ( e ) Blimp-1 expression was also determined as in d . The horizontal bars of each graph indicate the median value and are listed where appropriate for clarity. Statistical analyses were performed by Friedman nonparametric tests ( b , d , e ) and significance is denoted by asterisks where * P
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- Figure 3 Naive CD4 + T cells are converted to functional Tregs by tumor-infiltrating DCs and tumor conditioned medium (CM). (A-C) Naive CD4 + T cells from peripheral blood of patients with invasive breast carcinoma were co-cultured with or without autologous pDCs isolated from tumor (TI) or peripheral blood (PB) for 9 days in the presence or absence of 30% CM from autologous tumor slices or adjacent normal tissue slices. (A , B) Non-adherent cells from co-cultures were stained for CD3, CD4, CD25 and intracellular Foxp3, and analyzed by flow cytometry. Representative plots of gated CD3 + CD4 + cells (A) and quantification of percentage of Foxp3 + CD25 + cells among CD3 + CD4 + cells (B) are shown (mean +- SEM, n = 19; * P < 0.05, ** P < 0.01, *** P < 0.001 by Student's t -test). (C) Expression of Treg-associated genes, assessed by qRT-PCR normalized to GAPDH , in sorted CD4 + T cells, relative to expression in cultures without DCs or CM (mean +- SEM, n = 19; * P < 0.05, ** P < 0.01, *** P < 0.001 compared with naive CD4 + T cells cultured alone by Student's t -test). (D-G) Effect of naive CD4 + T cell-derived Tregs, obtained by co-culture with TI pDCs and tumor CM as above, on function of autologous tumor-specific CD8 + T cells. Tumor-specific CD8 + T cells were generated for each subject by stimulating autologous PB CD8 + T cells with autologous tumor lysate-pulsed autologous DCs. Tregs were recovered from co-cultures by magnetic sorting. (D) CFSE-labeled CD8 + T ce
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- Figure 6 In vivo knockdown of PITPNM3 in CD4 + T cells reverses immunosuppression and inhibits tumor progression in humanized mice. (A) Humanized mice bearing palpable MDA-MB-231 orthotopic xenografts were intraperitoneally injected daily for 14 days with PBS, 1 nmol CD4-aptamer-control siRNA (AsiC-con) or CD4-aptamer-siRNA targeting PITPNM3 (sequence in A , AsiC-PI) to assess the role of PITPNM3 in TI Tregs, and other T cells and tumor control. Experimental schematic is provided in Supplementary information, Figure S9A . (B) Representative immunoblots showing selective knockdown of PITPNM3 protein in PB CD4 + T cells, but not tumor xenografts ( n = 3). (C) PITPNM3 knockdown did not affect the distribution of human CD45 + hematopoietic cells, CD4 + and CD8 + T cells, and CD14 + monocytes in the peripheral blood of humanized mice. Representative flow plots are shown ( n = 3). (D , E) Effect of PITPNM3 knockdown on TI naive CD4 + , Tregs and CD8 + T cell numbers, and apoptosis by TUNEL assay in xenografts. D shows representative immunofluorescence microscopy images. Top row indicates CD4 + naive T cells by arrows; the second row indicates CD4 + CD45RO + Foxp3 - CD4 + memory T cells (yellow arrows) and Foxp3 + Tregs (white arrows). Scale bar, 50 mum. E shows number of cells of each subtype/high power field in eight mice ( ** P < 0.01, *** P < 0.001 compared to PBS group by Student's t -test). (F) Flow cytometry analysis of gated human CD3 + CD4 + cells isolated from xenogra
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