13-4776-80

antibody from Invitrogen Antibodies
Targeting: FOXP3 AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID
Provider product page for 13-4776-80
Western blot
Immunohistochemistry
Flow cytometry

Antibody data

Product number
13-4776-80 - Provider product page
Provider
Invitrogen Antibodies
Product name
Anti-FOXP3 Monoclonal Antibody (PCH101), Biotin, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: eBioscience offers a panel of monoclonal antibodies to different epitopes of human Foxp3, providing useful tools for investigating the complete expression pattern of Foxp3 at the protein level, and discerning the precise subsets of Foxp3^+ cells. The PCH101 antibody reacts with the amino terminus of human foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells. Intracellular staining of human peripheral blood mononuclear cells (PBMCs) with PCH101 antibody using the anti-human Foxp3 Staining Set and protocol reveals approximately 0.5-4% of lymphocytes staining, with the majority of staining occurring in the CD25^bright population. This is subject to donor variability. PCH101 crossreacts with rhesus, chimpanzee and cynomolgus. We recommend the use of CD4 (OKT4, cat. 11-0048, or RPA-T4, cat. 11-0049, depending on the species) and CD25 (BC96, cat. 17-0259). Applications Reported: This PCH101 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, western blotting, immunohistochemical staining of frozen and formalin-fixed paraffin embedded tissue sections. Applications Tested: This PCH101 antibody has been tested by intracellular flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This antibody can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Rat
Conjugate
Biotin
Isotype
IgG
Antibody clone number
PCH101
Vial size
25 µg
Concentration
0.5 mg/mL
Storage
4° C, store in dark, DO NOT FREEZE!
FOXP3 protein structure - 13-4776-80 shown in red.