13-4776-80
antibody from Invitrogen Antibodies
Targeting: FOXP3
AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID
Antibody data
- Antibody Data
- Antigen structure
- References [36]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 13-4776-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-FOXP3 Monoclonal Antibody (PCH101), Biotin, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: eBioscience offers a panel of monoclonal antibodies to different epitopes of human Foxp3, providing useful tools for investigating the complete expression pattern of Foxp3 at the protein level, and discerning the precise subsets of Foxp3^+ cells. The PCH101 antibody reacts with the amino terminus of human foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells. Intracellular staining of human peripheral blood mononuclear cells (PBMCs) with PCH101 antibody using the anti-human Foxp3 Staining Set and protocol reveals approximately 0.5-4% of lymphocytes staining, with the majority of staining occurring in the CD25^bright population. This is subject to donor variability. PCH101 crossreacts with rhesus, chimpanzee and cynomolgus. We recommend the use of CD4 (OKT4, cat. 11-0048, or RPA-T4, cat. 11-0049, depending on the species) and CD25 (BC96, cat. 17-0259). Applications Reported: This PCH101 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, western blotting, immunohistochemical staining of frozen and formalin-fixed paraffin embedded tissue sections. Applications Tested: This PCH101 antibody has been tested by intracellular flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This antibody can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- PCH101
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references IL-6 receptor blockade corrects defects of XIAP-deficient regulatory T cells.
Fc Effector Function Contributes to the Activity of Human Anti-CTLA-4 Antibodies.
HDAC inhibition potentiates immunotherapy in triple negative breast cancer.
Blocking the recruitment of naive CD4+ T cells reverses immunosuppression in breast cancer.
Equilibrium of Treg/Th17 cells of peripheral blood in syphilitic patients with sero-resistance.
Pulmonary sarcoidosis is associated with high-level inducible co-stimulator (ICOS) expression on lung regulatory T cells--possible implications for the ICOS/ICOS-ligand axis in disease course and resolution.
Human Head and Neck Squamous Cell Carcinoma-Associated Semaphorin 4D Induces Expansion of Myeloid-Derived Suppressor Cells.
Development of Type 2, But Not Type 1, Leprosy Reactions is Associated with a Severe Reduction of Circulating and In situ Regulatory T-Cells.
Glycolysis controls the induction of human regulatory T cells by modulating the expression of FOXP3 exon 2 splicing variants.
Isolation of human antigen-specific regulatory T cells with high suppressive function.
Tumor-infiltrating immune cell profiles and their change after neoadjuvant chemotherapy predict response and prognosis of breast cancer.
Human T cells upregulate CD69 after coculture with xenogeneic genetically-modified pig mesenchymal stromal cells.
T regulatory cells and related immunoregulatory factors in polymorphic light eruption following ultraviolet A1 challenge.
Human Treg responses allow sustained recombinant adeno-associated virus-mediated transgene expression.
Impaired function of regulatory T cells in cord blood of children of allergic mothers.
Increased expression of regulatory T cells and down-regulatory molecules in lepromatous leprosy.
Induction of endometriosis alters the peripheral and endometrial regulatory T cell population in the non-human primate.
The vaccine-site microenvironment induced by injection of incomplete Freund's adjuvant, with or without melanoma peptides.
Intra-graft expression of genes involved in iron homeostasis predicts the development of operational tolerance in human liver transplantation.
OMIP-006: phenotypic subset analysis of human T regulatory cells via polychromatic flow cytometry.
Engagement of TLR2 reverses the suppressor function of conjunctiva CD4+CD25+ regulatory T cells and promotes herpes simplex virus epitope-specific CD4+CD25- effector T cell responses.
Thymus-derived rather than tumor-induced regulatory T cells predominate in brain tumors.
CD40 signalling induces IL-10-producing, tolerogenic dendritic cells.
Safety and T cell modulating effects of high dose vitamin D3 supplementation in multiple sclerosis.
CD4(+) regulatory T cells in a cynomolgus macaque model of Mycobacterium tuberculosis infection.
Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells.
IL-15 and dermal fibroblasts induce proliferation of natural regulatory T cells isolated from human skin.
FOXP3 regulates TLR10 expression in human T regulatory cells.
IL-2 and IL-15 each mediate de novo induction of FOXP3 expression in human tumor antigen-specific CD8 T cells.
Mucosal but not peripheral FOXP3+ regulatory T cells are highly increased in untreated HIV infection and normalize after suppressive HAART.
IL-2 administration increases CD4+ CD25(hi) Foxp3+ regulatory T cells in cancer patients.
Foxp3+CD4+CD25+ T cells control virus-specific memory T cells in chimpanzees that recovered from hepatitis C.
Depletion of alloreactive T cells via CD69: implications on antiviral, antileukemic and immunoregulatory T lymphocytes.
Cutting edge: direct suppression of B cells by CD4+ CD25+ regulatory T cells.
Cutting edge: direct suppression of B cells by CD4+ CD25+ regulatory T cells.
Human CD4+ T cells express TLR5 and its ligand flagellin enhances the suppressive capacity and expression of FOXP3 in CD4+CD25+ T regulatory cells.
Hsieh WC, Hsu TS, Chang YJ, Lai MZ
Nature communications 2018 Jan 31;9(1):463
Nature communications 2018 Jan 31;9(1):463
Fc Effector Function Contributes to the Activity of Human Anti-CTLA-4 Antibodies.
Arce Vargas F, Furness AJS, Litchfield K, Joshi K, Rosenthal R, Ghorani E, Solomon I, Lesko MH, Ruef N, Roddie C, Henry JY, Spain L, Ben Aissa A, Georgiou A, Wong YNS, Smith M, Strauss D, Hayes A, Nicol D, O'Brien T, Mårtensson L, Ljungars A, Teige I, Frendéus B, TRACERx Melanoma., TRACERx Renal., TRACERx Lung consortia., Pule M, Marafioti T, Gore M, Larkin J, Turajlic S, Swanton C, Peggs KS, Quezada SA
Cancer cell 2018 Apr 9;33(4):649-663.e4
Cancer cell 2018 Apr 9;33(4):649-663.e4
HDAC inhibition potentiates immunotherapy in triple negative breast cancer.
Terranova-Barberio M, Thomas S, Ali N, Pawlowska N, Park J, Krings G, Rosenblum MD, Budillon A, Munster PN
Oncotarget 2017 Dec 26;8(69):114156-114172
Oncotarget 2017 Dec 26;8(69):114156-114172
Blocking the recruitment of naive CD4+ T cells reverses immunosuppression in breast cancer.
Su S, Liao J, Liu J, Huang D, He C, Chen F, Yang L, Wu W, Chen J, Lin L, Zeng Y, Ouyang N, Cui X, Yao H, Su F, Huang JD, Lieberman J, Liu Q, Song E
Cell research 2017 Apr;27(4):461-482
Cell research 2017 Apr;27(4):461-482
Equilibrium of Treg/Th17 cells of peripheral blood in syphilitic patients with sero-resistance.
Zhao J, Ma J, Zhang X, Li Q, Yang X
Experimental and therapeutic medicine 2016 Jun;11(6):2300-2304
Experimental and therapeutic medicine 2016 Jun;11(6):2300-2304
Pulmonary sarcoidosis is associated with high-level inducible co-stimulator (ICOS) expression on lung regulatory T cells--possible implications for the ICOS/ICOS-ligand axis in disease course and resolution.
Sakthivel P, Grunewald J, Eklund A, Bruder D, Wahlström J
Clinical and experimental immunology 2016 Feb;183(2):294-306
Clinical and experimental immunology 2016 Feb;183(2):294-306
Human Head and Neck Squamous Cell Carcinoma-Associated Semaphorin 4D Induces Expansion of Myeloid-Derived Suppressor Cells.
Younis RH, Han KL, Webb TJ
Journal of immunology (Baltimore, Md. : 1950) 2016 Feb 1;196(3):1419-29
Journal of immunology (Baltimore, Md. : 1950) 2016 Feb 1;196(3):1419-29
Development of Type 2, But Not Type 1, Leprosy Reactions is Associated with a Severe Reduction of Circulating and In situ Regulatory T-Cells.
Vieira AP, Trindade MÂ, Pagliari C, Avancini J, Sakai-Valente NY, Duarte AJ, Benard G
The American journal of tropical medicine and hygiene 2016 Apr;94(4):721-7
The American journal of tropical medicine and hygiene 2016 Apr;94(4):721-7
Glycolysis controls the induction of human regulatory T cells by modulating the expression of FOXP3 exon 2 splicing variants.
De Rosa V, Galgani M, Porcellini A, Colamatteo A, Santopaolo M, Zuchegna C, Romano A, De Simone S, Procaccini C, La Rocca C, Carrieri PB, Maniscalco GT, Salvetti M, Buscarinu MC, Franzese A, Mozzillo E, La Cava A, Matarese G
Nature immunology 2015 Nov;16(11):1174-84
Nature immunology 2015 Nov;16(11):1174-84
Isolation of human antigen-specific regulatory T cells with high suppressive function.
Noyan F, Lee YS, Zimmermann K, Hardtke-Wolenski M, Taubert R, Warnecke G, Knoefel AK, Schulde E, Olek S, Manns MP, Jaeckel E
European journal of immunology 2014 Sep;44(9):2592-602
European journal of immunology 2014 Sep;44(9):2592-602
Tumor-infiltrating immune cell profiles and their change after neoadjuvant chemotherapy predict response and prognosis of breast cancer.
García-Martínez E, Gil GL, Benito AC, González-Billalabeitia E, Conesa MA, García García T, García-Garre E, Vicente V, Ayala de la Peña F
Breast cancer research : BCR 2014 Nov 29;16(6):488
Breast cancer research : BCR 2014 Nov 29;16(6):488
Human T cells upregulate CD69 after coculture with xenogeneic genetically-modified pig mesenchymal stromal cells.
Li J, Andreyev O, Chen M, Marco M, Iwase H, Long C, Ayares D, Shen Z, Cooper DK, Ezzelarab MB
Cellular immunology 2013 Sep-Oct;285(1-2):23-30
Cellular immunology 2013 Sep-Oct;285(1-2):23-30
T regulatory cells and related immunoregulatory factors in polymorphic light eruption following ultraviolet A1 challenge.
Gambichler T, Terras S, Kampilafkos P, Kreuter A, Skrygan M
The British journal of dermatology 2013 Dec;169(6):1288-94
The British journal of dermatology 2013 Dec;169(6):1288-94
Human Treg responses allow sustained recombinant adeno-associated virus-mediated transgene expression.
Mueller C, Chulay JD, Trapnell BC, Humphries M, Carey B, Sandhaus RA, McElvaney NG, Messina L, Tang Q, Rouhani FN, Campbell-Thompson M, Fu AD, Yachnis A, Knop DR, Ye GJ, Brantly M, Calcedo R, Somanathan S, Richman LP, Vonderheide RH, Hulme MA, Brusko TM, Wilson JM, Flotte TR
The Journal of clinical investigation 2013 Dec;123(12):5310-8
The Journal of clinical investigation 2013 Dec;123(12):5310-8
Impaired function of regulatory T cells in cord blood of children of allergic mothers.
Hrdý J, Kocourková I, Prokešová L
Clinical and experimental immunology 2012 Oct;170(1):10-7
Clinical and experimental immunology 2012 Oct;170(1):10-7
Increased expression of regulatory T cells and down-regulatory molecules in lepromatous leprosy.
Palermo ML, Pagliari C, Trindade MA, Yamashitafuji TM, Duarte AJ, Cacere CR, Benard G
The American journal of tropical medicine and hygiene 2012 May;86(5):878-83
The American journal of tropical medicine and hygiene 2012 May;86(5):878-83
Induction of endometriosis alters the peripheral and endometrial regulatory T cell population in the non-human primate.
Braundmeier A, Jackson K, Hastings J, Koehler J, Nowak R, Fazleabas A
Human reproduction (Oxford, England) 2012 Jun;27(6):1712-22
Human reproduction (Oxford, England) 2012 Jun;27(6):1712-22
The vaccine-site microenvironment induced by injection of incomplete Freund's adjuvant, with or without melanoma peptides.
Harris RC, Chianese-Bullock KA, Petroni GR, Schaefer JT, Brill LB 2nd, Molhoek KR, Deacon DH, Patterson JW, Slingluff CL Jr
Journal of immunotherapy (Hagerstown, Md. : 1997) 2012 Jan;35(1):78-88
Journal of immunotherapy (Hagerstown, Md. : 1997) 2012 Jan;35(1):78-88
Intra-graft expression of genes involved in iron homeostasis predicts the development of operational tolerance in human liver transplantation.
Bohne F, Martínez-Llordella M, Lozano JJ, Miquel R, Benítez C, Londoño MC, Manzia TM, Angelico R, Swinkels DW, Tjalsma H, López M, Abraldes JG, Bonaccorsi-Riani E, Jaeckel E, Taubert R, Pirenne J, Rimola A, Tisone G, Sánchez-Fueyo A
The Journal of clinical investigation 2012 Jan;122(1):368-82
The Journal of clinical investigation 2012 Jan;122(1):368-82
OMIP-006: phenotypic subset analysis of human T regulatory cells via polychromatic flow cytometry.
Murdoch DM, Staats JS, Weinhold KJ
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2012 Apr;81(4):281-3
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2012 Apr;81(4):281-3
Engagement of TLR2 reverses the suppressor function of conjunctiva CD4+CD25+ regulatory T cells and promotes herpes simplex virus epitope-specific CD4+CD25- effector T cell responses.
Dasgupta G, Chentoufi AA, You S, Falatoonzadeh P, Urbano LA, Akhtarmalik A, Nguyen K, Ablabutyan L, Nesburn AB, BenMohamed L
Investigative ophthalmology & visual science 2011 May 17;52(6):3321-33
Investigative ophthalmology & visual science 2011 May 17;52(6):3321-33
Thymus-derived rather than tumor-induced regulatory T cells predominate in brain tumors.
Wainwright DA, Sengupta S, Han Y, Lesniak MS
Neuro-oncology 2011 Dec;13(12):1308-23
Neuro-oncology 2011 Dec;13(12):1308-23
CD40 signalling induces IL-10-producing, tolerogenic dendritic cells.
Tuettenberg A, Fondel S, Steinbrink K, Enk AH, Jonuleit H
Experimental dermatology 2010 Jan;19(1):44-53
Experimental dermatology 2010 Jan;19(1):44-53
Safety and T cell modulating effects of high dose vitamin D3 supplementation in multiple sclerosis.
Smolders J, Peelen E, Thewissen M, Cohen Tervaert JW, Menheere P, Hupperts R, Damoiseaux J
PloS one 2010 Dec 13;5(12):e15235
PloS one 2010 Dec 13;5(12):e15235
CD4(+) regulatory T cells in a cynomolgus macaque model of Mycobacterium tuberculosis infection.
Green AM, Mattila JT, Bigbee CL, Bongers KS, Lin PL, Flynn JL
The Journal of infectious diseases 2010 Aug 15;202(4):533-41
The Journal of infectious diseases 2010 Aug 15;202(4):533-41
Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells.
Nagata S, Ise T, Pastan I
Journal of immunology (Baltimore, Md. : 1950) 2009 Jun 15;182(12):7518-26
Journal of immunology (Baltimore, Md. : 1950) 2009 Jun 15;182(12):7518-26
IL-15 and dermal fibroblasts induce proliferation of natural regulatory T cells isolated from human skin.
Clark RA, Kupper TS
Blood 2007 Jan 1;109(1):194-202
Blood 2007 Jan 1;109(1):194-202
FOXP3 regulates TLR10 expression in human T regulatory cells.
Bell MP, Svingen PA, Rahman MK, Xiong Y, Faubion WA Jr
Journal of immunology (Baltimore, Md. : 1950) 2007 Aug 1;179(3):1893-900
Journal of immunology (Baltimore, Md. : 1950) 2007 Aug 1;179(3):1893-900
IL-2 and IL-15 each mediate de novo induction of FOXP3 expression in human tumor antigen-specific CD8 T cells.
Ahmadzadeh M, Antony PA, Rosenberg SA
Journal of immunotherapy (Hagerstown, Md. : 1997) 2007 Apr;30(3):294-302
Journal of immunotherapy (Hagerstown, Md. : 1997) 2007 Apr;30(3):294-302
Mucosal but not peripheral FOXP3+ regulatory T cells are highly increased in untreated HIV infection and normalize after suppressive HAART.
Epple HJ, Loddenkemper C, Kunkel D, Tröger H, Maul J, Moos V, Berg E, Ullrich R, Schulzke JD, Stein H, Duchmann R, Zeitz M, Schneider T
Blood 2006 Nov 1;108(9):3072-8
Blood 2006 Nov 1;108(9):3072-8
IL-2 administration increases CD4+ CD25(hi) Foxp3+ regulatory T cells in cancer patients.
Ahmadzadeh M, Rosenberg SA
Blood 2006 Mar 15;107(6):2409-14
Blood 2006 Mar 15;107(6):2409-14
Foxp3+CD4+CD25+ T cells control virus-specific memory T cells in chimpanzees that recovered from hepatitis C.
Manigold T, Shin EC, Mizukoshi E, Mihalik K, Murthy KK, Rice CM, Piccirillo CA, Rehermann B
Blood 2006 Jun 1;107(11):4424-32
Blood 2006 Jun 1;107(11):4424-32
Depletion of alloreactive T cells via CD69: implications on antiviral, antileukemic and immunoregulatory T lymphocytes.
Hartwig UF, Nonn M, Khan S, Meyer RG, Huber C, Herr W
Bone marrow transplantation 2006 Feb;37(3):297-305
Bone marrow transplantation 2006 Feb;37(3):297-305
Cutting edge: direct suppression of B cells by CD4+ CD25+ regulatory T cells.
Lim HW, Hillsamer P, Banham AH, Kim CH
Journal of immunology (Baltimore, Md. : 1950) 2005 Oct 1;175(7):4180-3
Journal of immunology (Baltimore, Md. : 1950) 2005 Oct 1;175(7):4180-3
Cutting edge: direct suppression of B cells by CD4+ CD25+ regulatory T cells.
Lim HW, Hillsamer P, Banham AH, Kim CH
Journal of immunology (Baltimore, Md. : 1950) 2005 Oct 1;175(7):4180-3
Journal of immunology (Baltimore, Md. : 1950) 2005 Oct 1;175(7):4180-3
Human CD4+ T cells express TLR5 and its ligand flagellin enhances the suppressive capacity and expression of FOXP3 in CD4+CD25+ T regulatory cells.
Crellin NK, Garcia RV, Hadisfar O, Allan SE, Steiner TS, Levings MK
Journal of immunology (Baltimore, Md. : 1950) 2005 Dec 15;175(12):8051-9
Journal of immunology (Baltimore, Md. : 1950) 2005 Dec 15;175(12):8051-9
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Surface staining of normal human peripheral blood cells with Anti-Human CD4 FITC (Product # 11-0049-42) (left) and Anti-Human CD25 APC (Product # 17-0259-42) (right), followed by intracellular staining with 0.25 µg of Anti-Human Foxp3 Biotin or Rat IgG2a K Isotype Control Biotin (Product # 13-4321-82) followed by Streptavidin PE (Product # 12-4317-87) using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Biotin