14-7979-82

antibody from Invitrogen Antibodies

Targeting: FOXP3 AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14-7979-82

Provider

Invitrogen Antibodies

Product name

FOXP3 Monoclonal Antibody (eBio7979 (221D/D3)), eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: eBioscience offers a panel of monoclonal antibodies to different epitopes of human and mouse Foxp3, providing useful tools for investigating the complete expression pattern of Foxp3 at the protein level, and discerning the precise subsets of Foxp3+ cells. Other antibodies to human Foxp3 include PCH101 (Product # 72-5776) and 236A/E7 (Product # 12-4777) . Additional antibodies to mouse Foxp3 include FJK-16s (Product # 72-5775) and NRRF-30 (Product # 12-4771) . The eBio7979 antibody reacts with human and mouse foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells. Please see our FAQ regarding the usage of eBioscience Foxp3 reagents. Applications Reported: This eBio7979 antibody has been reported for use in immunoblotting (WB). Applications Tested: This eBio7979 antibody has been tested by immunoblotting of normal human peripheral blood mononuclear cell lysates at 1-2 µg/mL. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

eBio7979 (221D/D3)

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

4° C

References

Submitted references

Mouse Double Minute 2 Homolog-Mediated Ubiquitination Facilitates Forkhead Box P3 Stability and Positively Modulates Human Regulatory T Cell Function.

Wang A, Yang M, Liang R, Zhu F, Zhu F, Liu X, Han Y, Lin R, Wang X, Li D, Li H, Yuan X, Zhao H, Li B
Frontiers in immunology 2020;11:1087

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Expression of FOXP3 in Canine Gliomas: Immunohistochemical Study of Tumor-Infiltrating Regulatory Lymphocytes.

Pi Castro D, José-López R, Fernández Flores F, Rabanal Prados RM, Mandara MT, Arús C, Pumarola Batlle M
Journal of neuropathology and experimental neurology 2020 Feb 1;79(2):184-193

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Changes in Expression of Multiple Checkpoint Molecules and Infiltration of Tumor Immune Cells after Neoadjuvant Chemotherapy in Gastric Cancer.

Yu Y, Ma X, Zhang Y, Zhang Y, Ying J, Zhang W, Zhong Q, Zhou A, Zeng Y
Journal of Cancer 2019;10(12):2754-2763

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Silencing of CD86 in dendritic cells by small interfering RNA regulates cytokine production in T cells from patients with allergic rhinitis in vitro.

Sun R, Yang Y, Gu Z, Tang X, Zhang C, Kou W, Wei P
Molecular medicine reports 2019 Oct;20(4):3893-3900

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Off-Target Deletion of Conditional Dbc1 Allele in the Foxp3(YFP-Cre) Mouse Line under Specific Setting.

Xie C, Zhu F, Wang J, Zhang W, Bellanti JA, Li B, Brand D, Olsen N, Zheng SG
Cells 2019 Oct 24;8(11)

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Time-resolved transcriptome and proteome landscape of human regulatory T cell (Treg) differentiation reveals novel regulators of FOXP3.

Schmidt A, Marabita F, Kiani NA, Gross CC, Johansson HJ, Éliás S, Rautio S, Eriksson M, Fernandes SJ, Silberberg G, Ullah U, Bhatia U, Lähdesmäki H, Lehtiö J, Gomez-Cabrero D, Wiendl H, Lahesmaa R, Tegnér J
BMC biology 2018 May 7;16(1):47

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Aneustat (OMN54) has aerobic glycolysis-inhibitory activity and also immunomodulatory activity as indicated by a first-generation PDX prostate cancer model.

Qu S, Xue H, Dong X, Lin D, Wu R, Nabavi N, Collins CC, Gleave ME, Gout PW, Wang Y
International journal of cancer 2018 Jul 15;143(2):419-429

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Ring finger protein 31-mediated atypical ubiquitination stabilizes forkhead box P3 and thereby stimulates regulatory T-cell function.

Zhu F, Yi G, Liu X, Zhu F, Zhao A, Wang A, Zhu R, Chen Z, Zhao B, Fang S, Yu X, Lin R, Liang R, Li D, Zhao W, Zhang Z, Guo W, Zhang S, Ge S, Fan X, Zhao G, Li B
The Journal of biological chemistry 2018 Dec 28;293(52):20099-20111

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Proximity Ligation Assay to Quantify Foxp3 Acetylation in Regulatory T Cells.

Jiao J, Han R, Hancock WW, Beier UH
Methods in molecular biology (Clifton, N.J.) 2017;1510:287-293

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Hypercholesterolemia Enhances T Cell Receptor Signaling and Increases the Regulatory T Cell Population.

Mailer RKW, Gisterå A, Polyzos KA, Ketelhuth DFJ, Hansson GK
Scientific reports 2017 Nov 15;7(1):15655

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Foxp3 enhances HIF-1α target gene expression in human bladder cancer through decreasing its ubiquitin-proteasomal degradation.

Jou YC, Tsai YS, Lin CT, Tung CL, Shen CH, Tsai HT, Yang WH, Chang HI, Chen SY, Tzai TS
Oncotarget 2016 Oct 4;7(40):65403-65417

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Altered connexin 43 expression underlies age-dependent decrease of regulatory T cell suppressor function in nonobese diabetic mice.

Kuczma M, Wang CY, Ignatowicz L, Gourdie R, Kraj P
Journal of immunology (Baltimore, Md. : 1950) 2015 Jun 1;194(11):5261-71

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Responses of the spleen to intraamniotic lipopolysaccharide exposure in fetal sheep.

Kuypers E, Willems MG, Jellema RK, Kemp MW, Newnham JP, Delhaas T, Kallapur SG, Jobe AH, Wolfs TG, Kramer BW
Pediatric research 2015 Jan;77(1-1):29-35

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CD4+ T lymphocyte ablation prevents pancreatic carcinogenesis in mice.

Zhang Y, Yan W, Mathew E, Bednar F, Wan S, Collins MA, Evans RA, Welling TH, Vonderheide RH, di Magliano MP
Cancer immunology research 2014 May;2(5):423-35

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Chorioamnionitis-induced fetal gut injury is mediated by direct gut exposure of inflammatory mediators or by lung inflammation.

Wolfs TG, Kramer BW, Thuijls G, Kemp MW, Saito M, Willems MG, Senthamarai-Kannan P, Newnham JP, Jobe AH, Kallapur SG
American journal of physiology. Gastrointestinal and liver physiology 2014 Mar 1;306(5):G382-93

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Selective exposure of the fetal lung and skin/amnion (but not gastro-intestinal tract) to LPS elicits acute systemic inflammation in fetal sheep.

Kemp MW, Kannan PS, Saito M, Newnham JP, Cox T, Jobe AH, Kramer BW, Kallapur SG
PloS one 2013;8(5):e63355

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Markers of T cell infiltration and function associate with favorable outcome in vascularized high-grade serous ovarian carcinoma.

Townsend KN, Spowart JE, Huwait H, Eshragh S, West NR, Elrick MA, Kalloger SE, Anglesio M, Watson PH, Huntsman DG, Lum JJ
PloS one 2013;8(12):e82406

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Intraamniotic lipopolysaccharide exposure changes cell populations and structure of the ovine fetal thymus.

Kuypers E, Wolfs TG, Collins JJ, Jellema RK, Newnham JP, Kemp MW, Kallapur SG, Jobe AH, Kramer BW
Reproductive sciences (Thousand Oaks, Calif.) 2013 Aug;20(8):946-56

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Ovine fetal thymus response to lipopolysaccharide-induced chorioamnionitis and antenatal corticosteroids.

Kuypers E, Collins JJ, Jellema RK, Wolfs TG, Kemp MW, Nitsos I, Pillow JJ, Polglase GR, Newnham JP, Germeraad WT, Kallapur SG, Jobe AH, Kramer BW
PloS one 2012;7(5):e38257

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Intra-amniotic LPS and antenatal betamethasone: inflammation and maturation in preterm lamb lungs.

Kuypers E, Collins JJ, Kramer BW, Ofman G, Nitsos I, Pillow JJ, Polglase GR, Kemp MW, Newnham JP, Gavilanes AW, Nowacki R, Ikegami M, Jobe AH, Kallapur SG
American journal of physiology. Lung cellular and molecular physiology 2012 Feb 15;302(4):L380-9

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Pulmonary and systemic inflammatory responses to intra-amniotic IL-1α in fetal sheep.

Kallapur SG, Kramer BW, Nitsos I, Pillow JJ, Collins JJ, Polglase GR, Newnham JP, Jobe AH
American journal of physiology. Lung cellular and molecular physiology 2011 Sep;301(3):L285-95

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Connexin 43 signaling enhances the generation of Foxp3+ regulatory T cells.

Kuczma M, Lee JR, Kraj P
Journal of immunology (Baltimore, Md. : 1950) 2011 Jul 1;187(1):248-57

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FOXP3 orchestrates H4K16 acetylation and H3K4 trimethylation for activation of multiple genes by recruiting MOF and causing displacement of PLU-1.

Katoh H, Qin ZS, Liu R, Wang L, Li W, Li X, Wu L, Du Z, Lyons R, Liu CG, Liu X, Dou Y, Zheng P, Liu Y
Molecular cell 2011 Dec 9;44(5):770-84

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Repression of the genome organizer SATB1 in regulatory T cells is required for suppressive function and inhibition of effector differentiation.

Beyer M, Thabet Y, Müller RU, Sadlon T, Classen S, Lahl K, Basu S, Zhou X, Bailey-Bucktrout SL, Krebs W, Schönfeld EA, Böttcher J, Golovina T, Mayer CT, Hofmann A, Sommer D, Debey-Pascher S, Endl E, Limmer A, Hippen KL, Blazar BR, Balderas R, Quast T, Waha A, Mayer G, Famulok M, Knolle PA, Wickenhauser C, Kolanus W, Schermer B, Bluestone JA, Barry SC, Sparwasser T, Riley JL, Schultze JL
Nature immunology 2011 Aug 14;12(9):898-907

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TCR repertoire and Foxp3 expression define functionally distinct subsets of CD4+ regulatory T cells.

Kuczma M, Pawlikowska I, Kopij M, Podolsky R, Rempala GA, Kraj P
Journal of immunology (Baltimore, Md. : 1950) 2009 Sep 1;183(5):3118-29

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IL-6 deficiency attenuates murine diet-induced non-alcoholic steatohepatitis.

Mas E, Danjoux M, Garcia V, Carpentier S, Ségui B, Levade T
PloS one 2009 Nov 20;4(11):e7929

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Early life exposure to lipopolysaccharide suppresses experimental autoimmune encephalomyelitis by promoting tolerogenic dendritic cells and regulatory T cells.

Ellestad KK, Tsutsui S, Noorbakhsh F, Warren KG, Yong VW, Pittman QJ, Power C
Journal of immunology (Baltimore, Md. : 1950) 2009 Jul 1;183(1):298-309

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Neonatal bacillus Calmette-Guérin vaccination inhibits de novo allergic inflammatory response in mice via alteration of CD4+CD25+ T-regulatory cells.

Li Q, Shen HH
Acta pharmacologica Sinica 2009 Jan;30(1):125-33

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Tumor-infiltrating T cells correlate with NY-ESO-1-specific autoantibodies in ovarian cancer.

Milne K, Barnes RO, Girardin A, Mawer MA, Nesslinger NJ, Ng A, Nielsen JS, Sahota R, Tran E, Webb JR, Wong MQ, Wick DA, Wray A, McMurtrie E, Köbel M, Kalloger SE, Gilks CB, Watson PH, Nelson BH
PloS one 2008;3(10):e3409

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Notch1 and TGFbeta1 cooperatively regulate Foxp3 expression and the maintenance of peripheral regulatory T cells.

Samon JB, Champhekar A, Minter LM, Telfer JC, Miele L, Fauq A, Das P, Golde TE, Osborne BA
Blood 2008 Sep 1;112(5):1813-21

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IL-15 acts as a potent inducer of CD4(+)CD25(hi) cells expressing FOXP3.

Imamichi H, Sereti I, Lane HC
European journal of immunology 2008 Jun;38(6):1621-30

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Transcriptional regulation by Foxp3 is associated with direct promoter occupancy and modulation of histone acetylation.

Chen C, Rowell EA, Thomas RM, Hancock WW, Wells AD
The Journal of biological chemistry 2006 Dec 1;281(48):36828-34

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The origin of FOXP3-expressing CD4+ regulatory T cells: thymus or periphery.

Sakaguchi S
The Journal of clinical investigation 2003 Nov;112(9):1310-2

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoblotting of PBMC (Lane A), BALB/c splenocyte (Lane B), or recombinant human Foxp3 fusion protein (Lane C). Lysates were directly sonicated in Laemmli sample buffer and 10e6 cells/lane were loaded. Blots were probed with 1 µg/mL Anti-Human/Mouse Foxp3 Purified and revealed with Anti-Mouse IgG HRP. Foxp3 is detected at ~50 kDa with a reported splice variant in human tissue slightly lower.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

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Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 Immunohistochemical analysis of serous ovarian tumors showing cases with high (left) and low (right) scores for the following markers: (A,B) TIA-1; (C,D) FoxP3; and (E,F) CD25.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6 Effects of IL-6 deficiency on MCD diet-induced changes in liver expression of PPAR-gamma and Foxp3. Equal amounts of protein (50 ug) were analyzed by Western blotting (representative images on the left panels). Densitometric analysis of the blots is presented on the right panels. Data are means+-SEM; the number of animals is indicated into each column. Asterisks denote significant differences (* p

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6 Foxp3-positive cells in the thymus. Representative images for Foxp3 expression in the thymus are shown for controls ( A ), 14 d LPS ( B ), and 14 d LPS+7 d Beta group ( C ). D: The percentage of Foxp3-positive stained area in the thymic medulla decreased in the animals exposed to 14 days of LPS independent of Beta treatment. Magnification 200x; magnification insert: 400x. * p

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 Representative images of pre-NACT (A) and post-NACT (B) marker expression (200x, and 400x for enlarged view). Immunofluorescence staining of CD4 (magenta), CD8 (red), PD-1 (orange), PD-L1 (yellow), TIM3 (pink), and FOXP3 (green) and DAPI staining of nuclear DNA (blue). Bar represents 100um.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5 The Foxp3 was expressed in the ovary, testis and fetus. ( a , b ) The Foxp3 protein level in the indicated tissue was tested by western blotting. ( c ) Foxp3 and Cre mRNA level in the indicated tissue was tested by qPCR. The data were represented as the mean +- standard deviation of three independent experiments. ( d ) The PCR product sizes of the fetus at embryonic day 14.5 in the progeny of male Dbc1 fl/fl mice crossed with female Foxp3 Cre/Cre mice or Cd4 Cre/Cre mice, using the primer indicated. The relative amount of the floxed Dbc1 and recombined Dbc1 of the fetus was tested by qPCR. For qPCR, the results were normalized to the DNA sample from the Dbc1 fl/+ mice or mouse #4 in ( a ). The panel of Foxp3 Cre shows the genotype of Foxp3 -IRES-YFP-Cre alleles. The panel of Cd4 Cre shows the genotype of Cd4 -Cre alleles. The data were represented as the mean +- standard deviation of three independent experiments.